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  • 1
    Electronic Resource
    Electronic Resource
    Molecular markers and protein quantities as genetic descriptors in maize. II. Prediction of performance of hybrids for forage traits (1995)
    Oxford, UK : Blackwell Publishing Ltd
    Plant breeding 114 (1995), S. 0 
    Details
    ISSN: 1439-0523
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: As it is related to the variability in genome expression, variability in protein quantities revealed by two-dimensional electrophoresis was proposed for describing phenotypic diversity. The objective of this study was to compare the predictive power of different genetic distances derived from molecular markers and from protein quantitative variations in a diallel of 210 hybrids among 21 maize inbred lines (Zea mays L.) of various origin. The lines were characterized for: 1. 142 markers resulting from the analysis of enzyme, RFLP, and protein-structure polymorphism; and 2. The variation in relative quantities of 190 proteins. The hybrids were evaluated for six forage traits in four environments. Correlations between the genetic distances computed for 142 marker loci and hybrid performances were moderate to high in diallels using crosses between related lines. Genetic distances based on protein quantities showed, in most cases, similar or lower correlations. Distance measures were not useful as predictors of hybrid performance for crosses between unrelated lines. Protein quantities were better for revealing specific genotypes.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1111/j.1439-0523.1995.tb00825.x
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  • 2
    Electronic Resource
    Electronic Resource
    Silver staining of proteins: Standardized procedure for two-dimensional gels bound to polyester sheets
    Amsterdam : Elsevier
    Analytical Biochemistry 155 (1986), S. 45-50 
    Details
    ISSN: 0003-2697
    Keywords: polyester sheets ; proteins ; silver staining ; two-dimensional polyacrylamide gel electrophoresis
    Source: Elsevier Journal Backfiles on ScienceDirect 1907 - 2002
    Topics: Biology , Chemistry and Pharmacology
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1016/0003-2697(86)90222-8
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  • 3
    Electronic Resource
    Electronic Resource
    Observation de la transition interdite 61S0-3P0 pour l'isotope pair du mercure 202Hg dans un champ magnetique
    Amsterdam : Elsevier
    Optics Communications 6 (1972), S. 261-263 
    Details
    ISSN: 0030-4018
    Source: Elsevier Journal Backfiles on ScienceDirect 1907 - 2002
    Topics: Physics
    Type of Medium: Electronic Resource
    URL: http://linkinghub.elsevier.com/retrieve/pii/0030-4018(72)90189-7
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  • 4
    Electronic Resource
    Electronic Resource
    Action d'un champ magnetique sur le taux de polarisation de la raie interdite 2655,6 A (61S0-63P0) du mercure
    Amsterdam : Elsevier
    Optics Communications 7 (1973), S. 386-389 
    Details
    ISSN: 0030-4018
    Source: Elsevier Journal Backfiles on ScienceDirect 1907 - 2002
    Topics: Physics
    Type of Medium: Electronic Resource
    URL: http://linkinghub.elsevier.com/retrieve/pii/0030-4018(73)90061-8
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  • 5
    Electronic Resource
    Electronic Resource
    Study on nuclear and cytoplasmic genome expression in wheat by two-dimensional gel electrophoresis (1984)
    Springer
    Theoretical and applied genetics 68 (1984), S. 335-345 
    Details
    ISSN: 1432-2242
    Keywords: Triticum aestivum ; 2D electrophoresis ; Developmental genetics ; Cytoplasmic inheritance ; Genetic regulation
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary Two-dimensional gel electrophoresis of denaturated proteins were performed at five developmental stages or organs (hereafter referred to as stage-organs) on two wheat lines with four different cytoplasms. Five hundred and fifty to 712 reproducible spots were scored depending on the stage-organ. Each stage-organ is unambiguously characterized and several types of control of protein quantity are recorded. Post-translational modifications are hypothetized and may sometimes be stagespecific. Two cytoplasmic patterns are found: one for the euplasmic lines with Triticum aestivum cytoplasm and one for the alloplasmic lines with Aegilops juvenalis, Ae. ventricosa and Ae. kotschyi cytoplasms. Cytoplasmic variation is observed for 28 spots showing position difference, all of which are probably products of the LS gene, and for four spots showing differences for regulation of protein quantity. Nuclear variation between ‘Chinese Spring’ and ‘Selkirk’ is found for 20 allelic differences and for 20 regulatory systems, the latter number being probably underestimated.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00267887
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  • 6
    Electronic Resource
    Electronic Resource
    Is the polymorphism of protein amounts related to phenotypic variability? A comparison of two-dimensional electrophoresis data with morphological traits in maize (1987)
    Springer
    Theoretical and applied genetics 74 (1987), S. 194-202 
    Details
    ISSN: 1432-2242
    Keywords: Maize ; Protein polymorphism ; Morphological variation ; Genetic distances ; Two-dimensional electrophoresis
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary The hypothesis that the quantitative variations in gene product levels could be a more important basis for morphological and adaptative change than the classical qualitative variability revealed by electrophoretic techniques was studied by comparing five maize lines from three sets of variables: (i) qualitative variations of proteins (presence/absence) revealed by two-dimensional polyacrylamide gel electrophoresis (2D PAGE), at a physiological seedling stage; (ii) quantitative variations in proteins (spots more or less intense) revealed by 2D PAGE, at the same physiological stage; (iii) general combining abilities of fourteen heritable, morphological or agronomical characters measured at various juvenile and adult stages. Distances between lines were defined, based on qualitative and quantitative variations of proteins. These distances do not appear to be correlated and do not give the same patterns of divergence between lines, as shown by principal coordinate analyses. Mahalanobis distances computed from the general combining abilities of the morphological characters are significantly correlated (r=0.75) to quantitative but not to qualitative distances. The comparison of the first planes of the principal coordinate analyses performed on the three kinds of distances clearly confirms this finding. Our results are discussed in connection with the possible genetic meaning of the two molecular distances and with the hypothesis that regulatory processes are primarily implicated in morphological variation.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00289968
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  • 7
    Electronic Resource
    Electronic Resource
    Association of protein amount polymorphism (PAP) among maize lines with performances of their hybrids (1991)
    Springer
    Theoretical and applied genetics 82 (1991), S. 552-560 
    Details
    ISSN: 1432-2242
    Keywords: Zea mays L ; Two-dimensional electrophoresis ; Protein amount ; Genetic variability ; Heterosis
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary It has been suggested that molecular foundations of phenotypic diversity reside in the variability of genome expression. This variability can be appraised through the polymorphism of individual protein amounts (PAP: protein amount polymorphism). Eight maize inbred lines and ten of their single-cross hybrids were analyzed by two-dimensional polyacrylamide gel electrophoresis in order to examine the potential of PAP for predicting hybrid vigor. The 28 possible pairs of lines were characterized for: (i) the number, H of expected heterozygous structural loci in their hybrid, in the sample of loci revealed by 2D-PAGE; (ii) four distance indices based on PAP; (iii) the hybrid values for five agromorphological characters measured in four different year/locations. For the subset of ten hybrids analyzed by 2D-PAGE, the number of cases of nonadditive inheritance (NA) was also counted. Whereas H appeared to be related neither to the PAP indices, nor to NA, nor to hybrid performances, PAP indices were correlated to NA, and both were positively associated to hybrid performances. The possibility that PAP is responsible for quantitative trait variation is discussed. This could result in the definition of biological predictors of heterosis.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00226790
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  • 8
    Electronic Resource
    Electronic Resource
    Restriction fragment length polymorphism analysis of loci associated with disease resistance genes and developmental traits in Pisum sativum L. (1994)
    Springer
    Theoretical and applied genetics 88 (1994), S. 17-27 
    Details
    ISSN: 1432-2242
    Keywords: Restriction fragment length polymorphism (RFLP) ; Pisum sativum L. ; Disease resistances ; Developmental traits ; Quantitative trait loci (QTL)
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract An F2 population of pea (Pisum sativum L.) consisting of 174 plants was analysed by restriction fragment length polymorphism (RFLP) and random amplified polymorphic DNA (RAPD) techniques. Ascochyta pisi race C resistance, plant height, flowering earliness and number of nodes were measured in order to map the genes responsible for their variation. We have constructed a partial linkage map including 3 morphological character genes, 4 disease resistance genes, 56 RFLP loci, 4 microsatellite loci and 2 RAPD loci. Molecular markers linked to each resistance gene were found: Fusarium wilt (6 cM from Fw), powdery mildew (11 cM from er) and pea common Mosaic virus (15 cM from mo). QTLs (quantitative traits loci) for Ascochyta pisi race C resistance were mapped, with most of the variation explained by only three chromosomal regions. The QTL with the largest effect, on chromosome 4, was also mapped using a qualitative, Mendelian approach. Another QTL displayed a transgressive segregation, i.e. the parental line that was susceptible to Ascochyta blight had a resistance allele at this QTL. Analysis of correlations between developmental traits in terms of QTL effects and positions suggested a common genetic control of the number of nodes and earliness, and a loose relationship between these traits and height.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00222388
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  • 9
    Electronic Resource
    Electronic Resource
    Molecular markers and protein quantities as genetic descriptors in maize. I. Genetic diversity among 21 inbred lines (1994)
    Springer
    Theoretical and applied genetics 89 (1994), S. 943-950 
    Details
    ISSN: 1432-2242
    Keywords: Zea mays L. ; Genetic distances ; Protein polymorphism ; Two-dimensional electrophoresis ; Restriction fragment length polymorphism
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Twenty-one maize (Zea mays L.) inbred lines were analysed using isozyme electrophoresis, restriction fragment length polymorphism (RFLP), and two-dimensional electrophoresis of denatured proteins (2-D PAGE). Our goal was (1) to assess the genetic variability among these lines which are potential progenitors for the development of forage maize hybrids in Europe, and (2) to compare the relationship pattern revealed by the polymorphism at marker loci with the one derived from the amount of protein variability assessed by computer-assisted analysis of the 2-D electrophoregrams. Fourteen markers were obtained from isozyme polymorphism, 84 from the restriction fragment length polymorphism, and 70 from protein shifts revealed by 2-D PAGE. The Rogers' distance computed on the set of molecular markers was the most efficient to describe the pedigree relationships between lines. Quantitative protein data gave a picture of relationships between lines clearly different from the monogenic markers. When unrelated pairs of lines were considered, the Rogers' distance was weakly correlated to distances based on quantitative variations in the amount of protein which may be consistent with their polygenic control and the occurrence of gene interactions.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00224522
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  • 10
    Electronic Resource
    Electronic Resource
    The maize two-dimensional gel protein database: towards an integrated genome analysis program (1996)
    Springer
    Theoretical and applied genetics 93 (1996), S. 997-1005 
    Details
    ISSN: 1432-2242
    Keywords: Maize ; Two-dimensional electrophoresis ; Coleoptile proteins ; Leaf proteins ; Protein database
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract This paper describes the first maize database of proteins separated by two-dimensional electrophoresis. Fifty-six coleoptile proteins and 18 leaf proteins from two maize lines were partially microsequenced. Thirty-six proteins (49%) displayed high similarity with database proteins. Nine of these proteins, representing five different functions, had never been described in maize. No conclusive function could be found for 45 polypeptides (61% of the microsequenced proteins). In addition, an alternative identification method, based on amino acid analysis, allowed candidates to be proposed for 17 proteins out of 44 additional proteins analyzed in the coleoptiles. These results are stored in a database which also includes, when available, genetic information about the chromosomal location of structural genes and regulatory factors of proteins. This database is being used in the context of a project on the genetic mapping of the expressed genome in maize.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00224104
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