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  • 1
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    Variability of 3 cytoplasmically encoded proteins in the Triticum genus (1988)
    Springer Nature
    Details
    Publication Date: 1988-02-01
    Print ISSN: 0018-067X
    Electronic ISSN: 1365-2540
    Topics: Biology
    Published by Springer Nature
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    PAPER CURRENT
  • 2
    Electronic Resource
    Electronic Resource
    Two-dimensional gel electrophoresis of proteins as a tool in wheat genetics
    Amsterdam : Elsevier
    Biochimie 69 (1987), S. 781-787 
    Details
    ISSN: 0300-9084
    Keywords: 2-D electrophoresis ; Triticum ; Triticum ; electrophorese bidimensionnelle ; gene localization ; gene regulation ; homeologie ; homeology ; localisation chromosomique ; regulation genetique
    Source: Elsevier Journal Backfiles on ScienceDirect 1907 - 2002
    Topics: Biology , Chemistry and Pharmacology
    Type of Medium: Electronic Resource
    URL: http://linkinghub.elsevier.com/retrieve/pii/0300-9084(87)90199-4
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    Paper (German National Licenses)
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  • 3
    Electronic Resource
    Electronic Resource
    Analysis of leaf proteins in late flowering mutants of Arabidopsis thaliana (1995)
    Oxford, UK : Blackwell Publishing Ltd
    Physiologia plantarum 93 (1995), S. 0 
    Details
    ISSN: 1399-3054
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Biology
    Notes: Late flowering monogenic mutants of Arabidopsis thaliana (L.) Heynh. at the loci co, gi, fca, fve, fwa, fha, fpa, fy and their corresponding wild type, Landsberg erecta, were analysed by two-dimensional gel electrophoresis. All plants were grown under continuous light and proteins were extracted from leaves of the same age (20-day-old). The polypeptide patterns of the mutants at the loci co, gi, fca, fve, fwa, fha, fpa, and Landsberg erecta were identical. The mutant at the fy locus showed a qualitative difference with Landsberg erecta. Crosses were made between this line and the wild type Landsberg erecta. F2 plants, resulting from autopollination of the hybrid, were analysed and showed no cosegregation between the observed protein and the flowering phenotype, indicating that these two lines differ by more than a single mutation.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1111/j.1399-3054.1995.tb02234.x
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  • 4
    Electronic Resource
    Electronic Resource
    Compared responses of poplar cuttings and in vitro raised shoots to short-term chilling treatments (2000)
    Springer
    Plant cell reports 19 (2000), S. 954-960 
    Details
    ISSN: 1432-203X
    Keywords: Key words Poplar ; Chilling ; Lignin ; Sucrose ; Protein
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract  In woody plants, chilling stress occurs during the early spring growth and can have important economic consequences. The aim of this study was to compare the reliability of two different experimental systems, 3-month-old softwood cuttings and in vitro-grown shoots, to study chilling effects in a poplar clone (Populus tremula×P. tremuloides cv. Muhs1). Different parameters were recorded: lignin content, sucrose concentration and protein and fresh weight variation, during a 2-week treatment at 10  °C. Two families of polypeptides of high molecular weight (110 and 116 kDa) were shown to accumulate in response to chilling in both cuttings and microshoots. For some of the parameters studied, i.e. appearance of some groups of polypeptides and reduction of fresh weight gain, both in vitro and ex vitro systems were suitable and produced similar results. In contrast, for some other observations, i.e. on sucrose concentration and lignin content, the systems led to different conclusions. While sucrose and lignin contents were shown to increase in cuttings submitted to chilling, no variation in lignin and only a small temporary peak of sucrose could be observed in microshoots kept under chilling. These parameters seem not to be suitable for studying the response of poplar in the in vitro system in such a short-term study.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/s002990000229
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  • 5
    Electronic Resource
    Electronic Resource
    Study on nuclear and cytoplasmic genome expression in wheat by two-dimensional gel electrophoresis (1984)
    Springer
    Theoretical and applied genetics 68 (1984), S. 335-345 
    Details
    ISSN: 1432-2242
    Keywords: Triticum aestivum ; 2D electrophoresis ; Developmental genetics ; Cytoplasmic inheritance ; Genetic regulation
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary Two-dimensional gel electrophoresis of denaturated proteins were performed at five developmental stages or organs (hereafter referred to as stage-organs) on two wheat lines with four different cytoplasms. Five hundred and fifty to 712 reproducible spots were scored depending on the stage-organ. Each stage-organ is unambiguously characterized and several types of control of protein quantity are recorded. Post-translational modifications are hypothetized and may sometimes be stagespecific. Two cytoplasmic patterns are found: one for the euplasmic lines with Triticum aestivum cytoplasm and one for the alloplasmic lines with Aegilops juvenalis, Ae. ventricosa and Ae. kotschyi cytoplasms. Cytoplasmic variation is observed for 28 spots showing position difference, all of which are probably products of the LS gene, and for four spots showing differences for regulation of protein quantity. Nuclear variation between ‘Chinese Spring’ and ‘Selkirk’ is found for 20 allelic differences and for 20 regulatory systems, the latter number being probably underestimated.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00267887
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  • 6
    Electronic Resource
    Electronic Resource
    Chromosomal localization of structural genes and regulators in wheat by 2D electrophoresis of ditelosomic lines (1985)
    Springer
    Theoretical and applied genetics 71 (1985), S. 31-38 
    Details
    ISSN: 1432-2242
    Keywords: Triticum aestivum ; 2D electrophoresis ; Gene localization ; Homoeologous genes ; Genetic regulation
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary Among the 782 spots observed in two-dimensional gel electrophoresis of denatured proteins from etiolated wheat shoots, 185 were found to be variable between the euploid and 26 ditelosomic lines of ‘Chinese Spring’. Thirty-five structural genes were located on 17 chromosome arms. Numerous intensity changes showing alterations in protein levels were observed and led to the following statements: 1) regulators are frequently found and can be assigned for a same polypeptide to various chromosome arms; 2) for most polypeptides homoeologous arms do not manifest similar effects; 3) nevertheless, when affecting the same polypeptide, homoeologous arms display in most cases identical regulatory effects; 4) gene dosage compensation is observed in only one out of four homoeoallelic situations.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00278250
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  • 7
    Electronic Resource
    Electronic Resource
    Regulatory effects of homoeologous chromosome arms on wheat proteins at two developmental stages (1986)
    Springer
    Theoretical and applied genetics 73 (1986), S. 246-251 
    Details
    ISSN: 1432-2242
    Keywords: Triticum aestivum ; 2D electrophoresis ; Gene localization ; Gene regulation ; Homoeology
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary Two-dimensional gel electrophoresis was conducted on denatured proteins of the 10-day-old first leaf (1F stage) of 18 homoeologous ditelosomic (DT) lines of wheat cultivar ‘Chinese Spring’. The observations, compared to the euploid control and relative to previous data found on 7-day-old etiolated seedlings (G7 stage) of the same lines lead to the following statements: 1) the structural genes of 24 spots can be assigned to 12 chromosome arms; 2) regulatory effects are completely different between the 1F and the G7 stages which may indicate that the regulation of protein amounts is often stage-specific; 3) no case of complete gene dosage compensation is observed among 4 groups of hypothesized homoeoallelic products; 4) homoeologous DT lines do not manifest similar effects which suggest the absence of homoeology for the detected regulatory effects.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00289281
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  • 8
    Electronic Resource
    Electronic Resource
    Cytoplasmic male sterility in Vicia faba L. (1982)
    Springer
    Theoretical and applied genetics 61 (1982), S. 47-52 
    Details
    ISSN: 1432-2242
    Keywords: Vicia faba ; Cytoplasmic male sterility ; Cytoplasmic inheritance ; Plasmon
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary In many higher plants, nucleo-cytoplasmic interactions lead to pollen abortion. In Vicia faba, cytoplasmic male sterility is unstable as the cytoplasm appears to shift from a sterile to a fertile state. In this report, five flower phenotypes are defined but the study is focussed on the progenies obtained from intermediate, semi-sterile plants with the same homozygous nuclear constitution during five successive generations. The results could be interpreted by quantitative modifications of at least four different kinds of cytoplasmic determinants.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00261509
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  • 9
    Electronic Resource
    Electronic Resource
    Parental genome expression in synthetic wheats (Triticum turgidum sp. × T. tauschii sp.) revealed by two-dimensional electrophoresis of seedling proteins (1987)
    Springer
    Theoretical and applied genetics 74 (1987), S. 218-223 
    Details
    ISSN: 1432-2242
    Keywords: Triticum ; 2D electrophoresis ; Amphiploid ; Homoeology ; Gene regulation
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary Two-dimensional gel electrophoresis was conducted on etiolated seedling proteins from two distinct amphiploids (ABD1, ABD2) and their parental lines (AB1, D1 and AB2, D2), AB1 and AB2 being used as female. On the amphiploid patterns were found all the parental spots except 8 D spots of which 3 are cytoplasmically encoded. One exceptional polypeptide observed in ABD1 was present neither in AB1 nor D1. The patterns fromt the amphiploids very closely resemble the co-electrophoresis done with 1/3 D protein extract and 2/3 AB protein extract. Thus it is very likely that for most gene products revealed the genomes act independently of each other.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00289972
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  • 10
    Electronic Resource
    Electronic Resource
    Study on nuclear and cytoplasmic genome expression in wheat by two-dimensional gel electrophoresis (1983)
    Springer
    Theoretical and applied genetics 66 (1983), S. 1-7 
    Details
    ISSN: 1432-2242
    Keywords: Triticum aestivum ; Aegilops species ; Alloplasmic lines ; 2D gel electrophoresis ; Cytoplasmic inheritance
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary In this first analysis the protein patterns obtained by two-dimensional gel electrophoresis of 8 day-old leaves from 18 alloplasmic wheat lines are compared. From 440 spots retained on the basis of their reproducibility, 36 proteins were observed to vary in different cytoplasms, allowing us to distinguish the T. aestivum cytoplasm from 5 Aegilops cytoplasms. Twenty-four of the 36 variable proteins could be structurally related to the large subunit of RuBPCase. Nuclear variation between 3 wheat varieties was observed for 14 proteins.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00281838
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