ISSN:
1574-6968
Source:
Blackwell Publishing Journal Backfiles 1879-2005
Topics:
Biology
Notes:
In a previous study, we reported the excess production of α-1,3-linked mannose residues with the complete disappearance of β-1,2-linked mannose residues in cell wall mannans of Candida albicans serotype A strain cells, which were grown in yeast extract-Sabouraud liquid medium at pH 2.0. In the present study, we examined the immunochemical reactivity of the same mannan of NIH A-207 with an enzyme-linked immunosorbent assay (ELISA) using several antisera to antigenic factors of the genus Candida (FAbs) and the structure of the mannan by two-dimensional homonuclear Hartmann-Hahn analysis. The ELISA showed that the mannan reacts to FAb 4 but not to FAbs 13b and 34, which are reported to be antibody factors against linear side chains containing an α-1,3-linked mannose residue. In the Hartmann-Hahn analysis, we found two branched side chains, Manα1–2Manα1–3[Manα1–6]Manα1-(2Manα1-)22Man and Manα1–3[Manα1–6]Manα1-(2Manα1-)22Man, instead of the previously reported linear side chains. The branched side chains are oversynthesized under acidic conditions.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1111/j.1574-6968.1997.tb10433.x
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