ISSN:
1662-8985
Source:
Scientific.Net: Materials Science & Technology / Trans Tech Publications Archiv 1984-2008
Topics:
Mechanical Engineering, Materials Science, Production Engineering, Mining and Metallurgy, Traffic Engineering, Precision Mechanics
Notes:
The study of inflammation is important in understanding the reaction betweenbiomaterials and the immune system. In the current study, human umbilical vein endothelial cells(ECV-304), cultured with PRMI-1640, were detached from cells cultured with the supernatant ofmacrophages which contained TNF-α and IL-1β because of stimulation by biomaterials. Cells werethen put into contact with multiple biomaterials with different biocompatibilities. Levels ofmonocyte chemoattractant protein-1 (MCP-1) were measured. The results suggested that level ofMCP-1 cultured with supernatant of macrophages was higher than PRMI-1640 in the samebiomaterials. And, exposure to the positive control, Polytetrafluoroethylene,Poly-Lactic-co-Glycolic acid and American NPG alloy, resulted in high expression of MCP-1 (p〈0.001) following cytokine stimulation. MCP-1 was also significantly expressed in β-TricalciumPhosphate and Calcium Phosphate Cement samples (p 〈 0.01). These data suggested that MCP-1was activated by both cytokines and biomaterials
Type of Medium:
Electronic Resource
URL:
http://www.tib-hannover.de/fulltexts/2011/0528/01/41/transtech_doi~10.4028%252Fwww.scientific.net%252FAMR.47-50.1423.pdf
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