Publication Date:
2012-06-28
Description:
An N-terminal fragment of human SHARPIN was recombinantly expressed inEscherichia coli, purified and crystallized. Crystals suitable for X-ray diffraction were obtained by a one-step optimization of seed dilution and protein concentration using a two-dimensional grid screen. The crystals belonged to the primitive tetragonal space groupP43212, with unit-cell parametersa = b = 61.55,c= 222.81 Å. Complete data sets were collected from native and selenomethionine-substituted protein crystals at 100 K to 2.6 and 2.0 Å resolution, respectively.
Print ISSN:
1744-3091
Topics:
Biology
,
Chemistry and Pharmacology
,
Geosciences
,
Physics
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