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  • 1
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    Population genetic study on Tenualosa Ilisha from Persian Gulf (Khouzestan region) (2018)
    Iranian Fisheries Science Research Institute | Tehran, Iran
    Details
    Publikationsdatum: 2021-05-19
    Beschreibung: Information on the genetic structure of fish populations is useful for identification of stocks, management for sustainable exploitation and preservation of genetic diversity. The most important objective in population genetics is discrimination between populations within their distribution areas. The invention of polymerase chain reaction has created new possibilities for exploration of these differences in fish populations. One of these PCRbased methods is Random Amplified Polymorphic DNA (RAPD) which uses short oligonucleotide primers of arbitrary sequences to amplify discrete regions of the genome. The most important features of this method are: obtaining a large number of polymorphic DNA bands using tiny amounts of DNA without necessity of cloning or previous knowledge of DNA sequence. Ilisha shad (Tenualosa ilisha) which is locally known as Soboor is an anadromous fish living in sea shores, estuaries and downstream of rivers and migrate to upstream for spawning. The aim of this research was to study genetic structure of soboor in Khuzestan waters, including Karoon, Arvadrood and Bahmashir rivers, Persian Gulf (Sea) as well as Iraqi samples (caught from Shat-Al-Arab river), by using RAPD technique. For this purpose, fifteen random decamer primers were initially applied on DNA samples of 4 individuals from each region. After optimizing PCR condition, nine primers with best results were selected from which 58 polymorphic loci were obtained on 60 specimens (12 specimens from each geographical region). RAPD data were obtained by scoring 1 and 0 for presence or absence of polymorphic bands, respectively. RAPDPLOT, RAPDDIST and POPGENE computer Software were used to analyze the RAPD data. Canonical discriminant analysis was deployed for statistical analysis of the data. Maximum and minimum genetic distances were found between samples from Iraq and Sea (0.2870) and Arvandrood and Bahmanshir (0.1042), respectively. The UPGMA dendrogram showed that the samples from Karoon and Sea form a clade whereas samples from Iraq, Arvandrood and Bahmanshir rivers form another clade suggesting the hypothesis that there are two Iranian and Iraqi populations of this species and these fish select their own specific river for spawning. According to this hypothesis the specimens from Sea would destine Karoon as their spawning river. On the other hand, two other separate groups could be corresponded to Tigris and Euphrate rivers in Iraq. Moreover, canonical discriminant analysis indicates that samples from four geographical regions are statistically different from each other and high correlation among data was found within each region (P〈0.01) suggesting that Tenualosa ilisha is a schooling species. According to the above two hypotheses and considering the distribution of specimens in phylogenetic tree it can be concluded that Bahmanshir river is a specific pathway for those fish heading Karoon river for spawning whereas Shat-Al-Arab population uses both Bahmanshir and Arvandrood rivers to reach Shat-Al-Arab. To verify these three hypotheses further studies are needed.
    Beschreibung: Iranian Fisheries Science Research Institute
    Beschreibung: Published
    Schlagwort(e): Population ; Genetic ; Tenualosa ilisha ; Population ; Polymerase Chain Reaction ; DNA ; RAPD ; PCR ; Spawning
    Repository-Name: AquaDocs
    Materialart: Report , Refereed
    Format: 87pp.
    Standort Signatur Erwartet Verfügbarkeit
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  • 2
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    Development and evaluation of the polymerase chain reaction (PCR) method for diagnosis of spring viremia of carp virus (SVCV) (2021)
    Shahid Chamran University of Ahvaz, Aquatic Animal Health
    In:  http://aquaticcommons.org/id/eprint/21035 | 17408 | 2016-08-31 07:26:07 | 21035 | Islamic Azad University, Science and Research Branch, Tehran
    Details
    Publikationsdatum: 2021-07-13
    Beschreibung: Aquaculture has been expanded rapidly to become a major commercial and food-producing sector worldwide in recent decade. In parallel, viral diseases rapidly spread among farms causing enormous economic losses. The accurate detection of pathogens at early stages of infection is a key point for disease control in aquaculture. Spring Viraemia of Carp Virus (SVCV) is a very severe pathogen of carp fishes in different parts of the world and is categorized as a reportable listed disease in the annual published list of World Organization for animal Health (OIE). The objective of this study was to develop and evaluate RT- PCR test for detecting SVC virus and also the sensitivity and specificity of this test. A semi nested RT- PCR was designed using combination of three primers: two external (SVCF , SVCR) and one internal (SVCS) primers which based on conserved region of G gen. The specificity of designed primers (only external ones) by examination on Viral Hemorrhagic Septicemia Virus (VHSV) and Infectious Hematopoietic Necrosis Virus (IHNV) was confirmed. For optimizing of the PCR test, primer concentration, primer annealing temperature, cycle number and Mgcl2 concentration were surveyed. Also for validity test, prevention of false negative and Assurance of its accuracy, a competitive internal control (mimic) designed and its suitable concentration was defined. Evaluation of the sensitivity of designed test were conducted first by comparing the different commercially available RNA isolation guidelines, two guidelines: isotiocyanate phenol–chloroform based protocols (RNX–Plus Iran, Iq2000 kit Taiwan ) and two column based protocols (Cinna pure RNA Iran , high pure viral RNA kit, Roche Germany ). The results indicated that the column based protocols (Roche method and Cinna pure), yield 36.77 ng/μl and 16/47 ng/μl RNA concentration respectively, which were significantly higher than other protocols(P〈0.05). Then for evaluation of extracted RNA sensitivity, Serial dilution of SVCV strain 56.70 grown in EPC (1.9×105 TCID50/ml) was examined To compare sensitivity. Extracted RNA from serial dilution with stone's primers and commercial IQ-2000 kit were examined simultaneously. The result indicated that designed semi- nested RT- PCR was able to recognize SVC virus to 10-4 dilution and stone's primer recognize to 10-3 dilution whereas Iq-2000 commercial kit did not recognized in any dilution. In high virus titer in designed test two DNA band (462 bp and 266 bp) produced, and by decreasing virus titer 462 bp was omitted. In low virus titer or lack of virus, just DNA band (mimic) 729 bp can propagate. After designing and optimizing PCR test, a total of 400 suspected cultured Cyprinus carpio with high mortality from 4 aquaculture zone of Khuzestan province were collected and tested for SVCV during 2012- 2013 using developed PCR method and IQ- 2000. The results indicated that SVC virus was not observed in samples using both methods.
    Beschreibung: PhD
    Beschreibung: Advisors:Mojtaba, Alishahi;Masoud Reza, Seyfi Abad Shapouri;counsellors:Mohedes, Qhasemi
    Schlagwort(e): Aquaculture ; Health
    Repository-Name: AquaDocs
    Materialart: thesis
    Format: application/pdf
    Format: application/pdf
    Format: 185
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  • 3
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    A survey on health status of marine fish in Bandar-e-Imam station (2021)
    Iranian Fisheries Science Research Institute | Tehran, Iran
    In:  http://aquaticcommons.org/id/eprint/25189 | 18721 | 2018-09-03 17:16:20 | 25189 | Iranian Fisheries Science Research Institute
    Details
    Publikationsdatum: 2021-07-16
    Beschreibung: Mariculture is one of the most important sub sector fisheries industry in Asia-pacific region. There are over 40 marine fish species commonly cultured, such as groupers (Epinephlus spp.), snappers (Lutjanus spp.) and Asian sea bass (Lattes calcarifer). But this industry in Southeast Asia experienced serious disease problem since the late 1980s. Khouzestan province has a coastal line about 200 km with many Creek and suitable area for Mariculture. Marine fish culture in Khouzestan province in floating net cages was successfully initiated in Ghazaleh Creek following development of Epinephlus coioides artificial seed production in the 1372. This study has been conducted since 1384 to 1387 in Bandar-e-Imam station. The aim of this research project was to determine the health management status in cage and hatchery, identification of marine fish pathogens (Bacteria, Fungi and parasite), examination of heavy metal in cages sediment and test the physico chemical factors of water in cages and hatchery. Different parts of broodstocks body and fingerlings including intestine, gills and body surface were examined. In this study, 18 Genus and species of bacteria such as Vibrio alginolyticus, Vibrio anguillarum, Vibrio splendidus, Vibrio vulnificus, Plesiomonas shigelloides, Aeromonas hydrophila, Aeromonas caviae, Pseudomonas sp. were diagnosed. Also 7 Genus and species of fungi such as: Aspergillus Niger, Aspergillus flavus, Aspergillus fumigatus, Penicillium sp. And Fusarium sp. Were isolated. In this survey following of disease appearance parasites such as; protozoans Amyloodinium sp. And Trichodina sp. In E.coioides and Acanthopagrus latus and Sparidentex hasta, monogean such as Benedenia sp. In E.coioides and S. hasta, isopoda probably Nerocila sp. In nasal cavity of E.coioides and copepods such as: Caligus sp. And Lernanthropus sp. In A.latus and S.hasta were identified. In this study, microorganisms (bacteria, fungi and parasites) isolated from three different marine fishes species, were reported for the first time in Iran. The range of the physico-chemical parameters of water in cages were: DO (5.42-10.24 ppm), BOD5 (1.51-10.24 ppm), No_2 (0.0197-0.89 ppm), No_3 (3.53-11.93 ppm), Po_4 (0.965-6.05 ppm), turbidity (4-58 NTU) and ammonia (0.0008-0.03 ppm). According to the results parameters such as nitrate, nitrite and turbidity were found more than standard levels. Heavy metals: Ni and Pb relatively high observed.
    Schlagwort(e): Biology ; Iran ; Khouzestan province ; Bandar-e-imam ; Mariculture ; Fisheries ; Species ; Epinephlus spp. ; Lutjanus spp. ; Lattes calcarifer ; Bacteria ; Fungi ; Parasite ; Aspergillus niger ; Aspergillus flavus ; Aspergillus fumigatus ; Penicillium sp. ; Fusarium sp. ; Vibrio alginolyticus ; Vibrio anguillarum ; Vibrio splendidus ; Vibrio vulnificus ; Plesiomonas shigelloides ; Aeromonas hydrophila ; Aeromonas caviae ; Pseudomonas sp. ; Grouper ; Snapper ; Sea bass
    Repository-Name: AquaDocs
    Materialart: monograph
    Format: application/pdf
    Format: application/pdf
    Format: 86
    Standort Signatur Erwartet Verfügbarkeit
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  • 4
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    Monitoring infectious agents in L.vannamei farms in choebdeh-Abadan (2021)
    Iranian Fisheries Science Research Institute | Tehran, Iran
    In:  http://aquaticcommons.org/id/eprint/25262 | 18721 | 2018-09-07 07:28:07 | 25262 | Iranian Fisheries Science Research Institute
    Details
    Publikationsdatum: 2021-07-16
    Beschreibung: The introduction of L. vannamei to Iran from Hawaii was initiated when high mortality occurred in shrimp farms of Abadan in 2002 than in Bushehr during summer of 2004. Inspection of L. vannamei for infectious agents (Bacteria , virus , fungi and parasite ) , determine total plate count and total vibrio in Bahmanshir Rivers, shrimps and farms water , examination physicochemical factors of farms water , Identification of carriers of wssv in wild shrimp and crabs , the main objectives of this study , which was held in 2007 . 540 shrimp samples (pl12 pl15) and subadults were collected from different shrimp farms in khouzestan province and then check for identity bacteria, fungi and parasite. Also 280 samples of L. vannamei , wild shrimps ( Metapenaeus af inis , Exopalaemon styliferus ) and crabs (Grapsus sp .and Sesarma sp. ) were Collected from Bahmanshir river for virology studies by PCR procedure (Iq2000 kit WSSV , IHHNV , TSV ) . 120 samples of L. vannamei for histopathology had been collected randomly and preserved in Davidson’s fixation and then transferred to 75% ethyl alcohol for storage. (Hepatopancreas gills). Physicochemical parameters of water in culture ponds comprising of pH DO, BOD, NO_3, NO_2, salinity. Total. Hardness and NH3 were measured all over culture period for 340 times. Finally it has been detected 10 genus and species of bacteria consisting of V . alginolyticus, V. parahaemolyticus, V. proteolyticus , plesiomonas shigelloides ; 6 genus and species of fungi specially Aspergilus niger , Asp.fumigatus and Asp.flavus and two genus of parasites vorticella sp. And Zoothamnium sp. were isolated. Crab (Sesarma sp.) were tested by using Iq2000 diagnostic kit for WSSV that positive for WSSV. There was a positive result (three viruses TSV, IHHNV and WSSV) for postlarvae and subadults of L.vannamei by using Iq2000 kit and IFRO kit (Internal kit). Histopathological studies have shown inclusion bodies of TSV, WSSV, IHHNV, MBV and HPV in various tissues. Results demonstrated the mean of vibrio count (0. 01×103 - 1.96 ×103) and tolal plate count (0 .21 ×103 - 14.25 ×10 3). physicochemical parameters of water were measured as follows : salinity (12.3 22.5 ppt ).BOD5 ( 1.98 10.21 ppm ) , DO (3.17 11.25 ppm ) , NH3(0.02 3.45 ppm ) and temperature (20 31. 5 c) . Keywords: L.vannamei, bacteria, virus, fungi, parasite Histo pathology - physicochemical parameters of water- Abadan khouzestan province.
    Schlagwort(e): Aquaculture ; Iran ; Khouzestan Province ; Abadan ; Choebdeh ; Bahmanshir River ; L.Vannamei ; Bacteria ; Virus ; Fungi ; Parasite ; Histopathology ; Physicochemical parameters of water ; Mortality ; Shrimp ; Metapenaeus affinis ; Exopalaemon styliferus ; Grapsus sp. ; Sesarma sp. ; Aspergilus niger ; Asp. Fumigatus ; Asp. Flavus ; Temperature
    Repository-Name: AquaDocs
    Materialart: monograph
    Format: application/pdf
    Format: application/pdf
    Format: 78
    Standort Signatur Erwartet Verfügbarkeit
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  • 5
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    Population genetic study on Tenualosa Ilisha from Persian Gulf (Khouzestan region) (2021)
    Iranian Fisheries Science Research Institute | Tehran, Iran
    In:  http://aquaticcommons.org/id/eprint/25524 | 18721 | 2018-10-05 08:38:24 | 25524 | Iranian Fisheries Science Research Institute
    Details
    Publikationsdatum: 2021-07-16
    Beschreibung: Information on the genetic structure of fish populations is useful for identification of stocks, management for sustainable exploitation and preservation of genetic diversity. The most important objective in population genetics is discrimination between populations within their distribution areas. The invention of polymerase chain reaction has created new possibilities for exploration of these differences in fish populations. One of these PCRbased methods is Random Amplified Polymorphic DNA (RAPD) which uses short oligonucleotide primers of arbitrary sequences to amplify discrete regions of the genome. The most important features of this method are: obtaining a large number of polymorphic DNA bands using tiny amounts of DNA without necessity of cloning or previous knowledge of DNA sequence. Ilisha shad (Tenualosa ilisha) which is locally known as Soboor is an anadromous fish living in sea shores, estuaries and downstream of rivers and migrate to upstream for spawning. The aim of this research was to study genetic structure of soboor in Khuzestan waters, including Karoon, Arvadrood and Bahmashir rivers, Persian Gulf (Sea) as well as Iraqi samples (caught from Shat-Al-Arab river), by using RAPD technique. For this purpose, fifteen random decamer primers were initially applied on DNA samples of 4 individuals from each region. After optimizing PCR condition, nine primers with best results were selected from which 58 polymorphic loci were obtained on 60 specimens (12 specimens from each geographical region). RAPD data were obtained by scoring 1 and 0 for presence or absence of polymorphic bands, respectively. RAPDPLOT, RAPDDIST and POPGENE computer Software were used to analyze the RAPD data. Canonical discriminant analysis was deployed for statistical analysis of the data. Maximum and minimum genetic distances were found between samples from Iraq and Sea (0.2870) and Arvandrood and Bahmanshir (0.1042), respectively. The UPGMA dendrogram showed that the samples from Karoon and Sea form a clade whereas samples from Iraq, Arvandrood and Bahmanshir rivers form another clade suggesting the hypothesis that there are two Iranian and Iraqi populations of this species and these fish select their own specific river for spawning. According to this hypothesis the specimens from Sea would destine Karoon as their spawning river. On the other hand, two other separate groups could be corresponded to Tigris and Euphrate rivers in Iraq. Moreover, canonical discriminant analysis indicates that samples from four geographical regions are statistically different from each other and high correlation among data was found within each region (P〈0.01) suggesting that Tenualosa ilisha is a schooling species. According to the above two hypotheses and considering the distribution of specimens in phylogenetic tree it can be concluded that Bahmanshir river is a specific pathway for those fish heading Karoon river for spawning whereas Shat-Al-Arab population uses both Bahmanshir and Arvandrood rivers to reach Shat-Al-Arab. To verify these three hypotheses further studies are needed.
    Schlagwort(e): Biology ; Fisheries ; Iran ; Persian Gulf ; Khouzestan Province ; Karoon River ; Bahmanshir River ; Arvandrood River ; Population ; Genetic ; Tenualosa ilisha ; Population ; Polymerase Chain Reaction ; DNA ; RAPD ; PCR ; Spawning
    Repository-Name: AquaDocs
    Materialart: monograph
    Format: application/pdf
    Format: application/pdf
    Format: 87
    Standort Signatur Erwartet Verfügbarkeit
    BibTip Andere fanden auch interessant ...
    ARTIKEL (OA)
  • 6
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    Unbekannt
    A survey on health status of marine fish in Bandar-e-imam station (2018)
    Iranian Fisheries Science Research Institute | Tehran, Iran
    Details
    Publikationsdatum: 2021-05-19
    Beschreibung: Mariculture is one of the most important sub sector fisheries industry in Asia-pacific region. There are over 40 marine fish species commonly cultured, such as groupers (Epinephlus spp.), snappers (Lutjanus spp.) and Asian sea bass (Lattes calcarifer). But this industry in Southeast Asia experienced serious disease problem since the late 1980s. Khouzestan province has a coastal line about 200 km with many Creek and suitable area for Mariculture. Marine fish culture in Khouzestan province in floating net cages was successfully initiated in Ghazaleh Creek following development of Epinephlus coioides artificial seed production in the 1372. This study has been conducted since 1384 to 1387 in Bandar-e-Imam station. The aim of this research project was to determine the health management status in cage and hatchery, identification of marine fish pathogens (Bacteria, Fungi and parasite), examination of heavy metal in cages sediment and test the physico chemical factors of water in cages and hatchery. Different parts of broodstocks body and fingerlings including intestine, gills and body surface were examined. In this study, 18 Genus and species of bacteria such as Vibrio alginolyticus, Vibrio anguillarum, Vibrio splendidus, Vibrio vulnificus, Plesiomonas shigelloides , Aeromonas hydrophila, Aeromonas caviae, Pseudomonas sp. Were diagnosed. Also 7 Genus and species of fungi such as: Aspergillus niger , Aspergillus flavus , Aspergillus fumigatus , Penicillium sp. And Fusarium sp. Were isolated. In this survey following of disease appearance parasites such as ; protozoans Amyloodinium sp. And Trichodina sp. In E.coioides and Acanthopagrus latus and Sparidentex hasta , monogean such as Benedenia sp. In E.coioides and S. hasta, isopoda probably Nerocila sp. In nasal cavity of E.coioides and copepods such as: Caligus sp. And Lernanthropus sp. In A.latus and S.hasta were identified. In this study, microorganisms (bacteria, fungi and parasites) isolated from three different marine fishes species, were reported for the first time in Iran. The range of the physico-chemical parameters of water in cages were: DO(5.42-10.24 ppm), BOD5 (1.51-10.24 ppm), No2(0.0197-0.89 ppm), No3(3.53-11.93 ppm), Po4(0.965-6.05 ppm), turbidity ( 4-58 NTU) and ammonia ( 0.0008-0.03 ppm). According to the results parameters such as nitrate, nitrite and turbidity were found more than standard levels. Heavy metals: Ni and Pb relatively high observed.
    Beschreibung: Iranian Fisheries Science Research Institute
    Beschreibung: Published
    Schlagwort(e): Mariculture ; Fisheries ; Species ; Epinephlus spp. ; Lutjanus spp. ; Lattes calcarifer ; Bacteria ; Fungi ; Parasite ; Aspergillus niger ; Aspergillus flavus ; Aspergillus fumigatus ; Penicillium sp. ; Fusarium sp. ; Vibrio alginolyticus ; Vibrio anguillarum ; Vibrio splendidus ; Vibrio vulnificus ; Plesiomonas shigelloides ; Aeromonas hydrophila ; Aeromonas caviae ; Pseudomonas sp. ; Grouper ; Snapper ; Sea bass
    Repository-Name: AquaDocs
    Materialart: Report , Refereed
    Format: 86pp.
    Standort Signatur Erwartet Verfügbarkeit
    BibTip Andere fanden auch interessant ...
    ARTIKEL (OA)
  • 7
    facet.materialart.
    Unbekannt
    Monitoring infectious agents in L.vannamei farms in choebdeh-Abadan (2018)
    Iranian Fisheries Science Research Institute | Tehran, Iran
    Details
    Publikationsdatum: 2021-05-19
    Beschreibung: The introduction of L. vannamei to Iran from Hawaii was initiated when high mortality occurred in shrimp farms of Abadan in 2002 then in Bushehr during summer of 2004. Inspection of L. vannamei for infectious agents (Bacteria , virus , fungi and parasite ) , determine total plate count and total vibrio in Bahmanshir Rivers, shrimps and farms water , examination physicochemical factors of farms water , Identification of carriers of wssv in wild shrimp and crabs , the main objectives of this study , which was held in 2007 . 540 shrimp samples (pl12 pl15) and subadults were collected from different shrimp farms in khouzestan province and then check for identity bacteria, fungi and parasite. Also 280 samples of L. vannamei , wild shrimps ( Metapenaeus af inis , Exopalaemon styliferus ) and crabs (Grapsus sp .and Sesarma sp. ) were Collected from Bahmanshir river for virology studies by PCR procedure (Iq2000 kit WSSV , IHHNV , TSV ) . 120 samples of L. vannamei for histopathology had been collected randomly and preserved in Davidson’s fixation and then transferred to 75% ethyl alcohol for storage. (Hepatopancreas gills). Physicochemical parameters of water in culture ponds comprising of pH DO, BOD, NO3, NO2, salinity. Total. Hardness and NH3 were measured all over culture period for 340 times. Finally it has been detected 10 genus and species of bacteria consisting of V . alginolyticus, V. parahaemolyticus, V. proteolyticus , plesiomonas shigelloides ; 6 genus and species of fungi specially Aspergilus niger , Asp.fumigatus and Asp.flavus and two genus of parasites vorticella sp. And Zoothamnium sp. were isolated. Crab (Sesarma sp.) were tested by using Iq2000 diagnostic kit for WSSV that positive for WSSV. There was a positive result (three viruses TSV, IHHNV and WSSV) for postlarvae and subadults of L.vannamei by using Iq2000 kit and IFRO kit (Internal kit). Histopathological studies have shown inclusion bodies of TSV, WSSV, IHHNV, MBV and HPV in various tissues. Results demonstrated the mean of vibrio count (0. 01×103 - 1.96 ×103) and tolal plate count (0 .21 ×103 - 14.25 ×10 3). physicochemical parameters of water were measured as follows : salinity (12.3 22.5 ppt ).BOD5 ( 1.98 10.21 ppm ) , DO (3.17 11.25 ppm ) , NH3(0.02 3.45 ppm ) and temperature (20 31. 5 c) . Keywords: L.vannamei, bacteria, virus, fungi, parasite Histo pathology - physicochemical parameters of water- Abadan khouzestan province.
    Beschreibung: Iranian Fisheries Science Research Institute
    Beschreibung: Published
    Schlagwort(e): Virology ; Histopathology ; L.Vannamei ; Bacteria ; Virus ; Fungi ; Parasite ; Histopathology ; Physicochemical parameters of water ; Mortality ; Shrimp ; Metapenaeus affinis ; Exopalaemon styliferus ; Grapsus sp. ; Sesarma sp. ; Aspergilus niger ; Asp. Fumigatus ; Asp. Flavus ; Temperature
    Repository-Name: AquaDocs
    Materialart: Report , Refereed
    Format: 78pp.
    Standort Signatur Erwartet Verfügbarkeit
    BibTip Andere fanden auch interessant ...
    ARTIKEL (OA)
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