ISSN:
1365-3040
Source:
Blackwell Publishing Journal Backfiles 1879-2005
Topics:
Biology
Notes:
Abstract. We have isolated a cDNA clone whose corresponding transcript encodes an apoprotein of a hydroxyproline-rich glycoprotein. Both infection of plants with fungal spores and treatment of suspension-cultured bean cells with a fungal elicitor preparation cause a rapid decrease of the level of this mRNA. Wounding of bean tissue, however, leads to a very rapid, transient induction of this transcript. In contrast, all bean hydroxyproline-rich glycoproteins described previously are induced both after infection with fungal spores and after mechanical damage. The protein encoded by this new clone contains a proline-rich domain of about 30 kilodaltons. Most of these proline residues are part of the characteristic peptides Ser-Pro4, Scr-Pro5 and Ser-Pro6, which are often tandemly repeated. These tandem-repeats are not found in any of the other bean hydroxyproline-rich glycoproteins, where almost all of the Ser-Pro4 units are integrated into higher order repetitive units of 16 amino acids. The gene is present at a single or low copy number in the haploid genome and exhibits an unusual codon usage bias for the amino acids proline and serine.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1111/j.1365-3040.1990.tb01310.x
Permalink