Publikationsdatum:
2008-04-15
Beschreibung:
Comparative genomics of nucleosome positions provides a powerful means for understanding how the organization of chromatin and the transcription machinery co-evolve. Here we produce a high-resolution reference map of H2A.Z and bulk nucleosome locations across the genome of the fly Drosophila melanogaster and compare it to that from the yeast Saccharomyces cerevisiae. Like Saccharomyces, Drosophila nucleosomes are organized around active transcription start sites in a canonical -1, nucleosome-free region, +1 arrangement. However, Drosophila does not incorporate H2A.Z into the -1 nucleosome and does not bury its transcriptional start site in the +1 nucleosome. At thousands of genes, RNA polymerase II engages the +1 nucleosome and pauses. How the transcription initiation machinery contends with the +1 nucleosome seems to be fundamentally different across major eukaryotic lines.〈br /〉〈br /〉〈a href="https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2735122/" target="_blank"〉〈img src="https://static.pubmed.gov/portal/portal3rc.fcgi/4089621/img/3977009" border="0"〉〈/a〉 〈a href="https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2735122/" target="_blank"〉This paper as free author manuscript - peer-reviewed and accepted for publication〈/a〉〈br /〉〈br /〉〈span class="detail_caption"〉Notes: 〈/span〉Mavrich, Travis N -- Jiang, Cizhong -- Ioshikhes, Ilya P -- Li, Xiaoyong -- Venters, Bryan J -- Zanton, Sara J -- Tomsho, Lynn P -- Qi, Ji -- Glaser, Robert L -- Schuster, Stephan C -- Gilmour, David S -- Albert, Istvan -- Pugh, B Franklin -- GM47477/GM/NIGMS NIH HHS/ -- HG004160/HG/NHGRI NIH HHS/ -- R01 HG004160/HG/NHGRI NIH HHS/ -- R01 HG004160-01A1/HG/NHGRI NIH HHS/ -- England -- Nature. 2008 May 15;453(7193):358-62. doi: 10.1038/nature06929. Epub 2008 Apr 13.〈br /〉〈span class="detail_caption"〉Author address: 〈/span〉Center for Gene Regulation, Department of Biochemistry and Molecular Biology, The Pennsylvania State University, University Park, Pennsylvania 16802, USA.〈br /〉〈span class="detail_caption"〉Record origin:〈/span〉 〈a href="http://www.ncbi.nlm.nih.gov/pubmed/18408708" target="_blank"〉PubMed〈/a〉
Schlagwort(e):
Animals
;
Conserved Sequence/genetics
;
Drosophila melanogaster/embryology/enzymology/*genetics
;
Gene Expression Regulation/genetics
;
Genes, Insect/genetics
;
Genome, Insect/*genetics
;
Histones/metabolism
;
Nucleosomes/*genetics/*metabolism
;
Promoter Regions, Genetic/genetics
;
RNA Polymerase II/metabolism
;
Saccharomyces cerevisiae/genetics
;
Transcription Initiation Site
;
Transcription, Genetic/genetics
Print ISSN:
0028-0836
Digitale ISSN:
1476-4687
Thema:
Biologie
,
Chemie und Pharmazie
,
Medizin
,
Allgemeine Naturwissenschaft
,
Physik
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