ISSN:
1573-5060
Keywords:
rapid fluorescent in situ hybridization
;
pre-annealing
;
barley (Hordeum vulgare)
;
chickpea (Cicer reticulatum)
;
white clover (Trifolium repens)
;
millet (Pennisetum glaucum)
;
rye (Secale cereale)
;
sugarcane (Saccharum officinarum)
;
wheat (Triticum aestivum)
;
Aegilops spp.
;
Leymus spp
Source:
Springer Online Journal Archives 1860-2000
Topics:
Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
Notes:
Summary A rapid fluorescent in situ hybridization protocol, which can be completed in one working day, has been utilised to visualize chromosome preparations of barley, chickpea, white clover, millet, rye, sugarcane, wheat, Aegilops spp. and Leymus spp. Both total genomic DNA and cloned nuclear rDNA were used as a probe. This protocol has the advantage of brevity and flexibility over more conventional approaches, and has made fluorescent in situ hybridization a routine cytological procedure.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1007/BF00015729
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