ISSN:
1573-0972
Keywords:
Anti-tumour activity
;
polysaccharide
;
Sarcoma-180 (S-180)
;
Tricholoma sp.
Source:
Springer Online Journal Archives 1860-2000
Topics:
Biology
,
Process Engineering, Biotechnology, Nutrition Technology
Notes:
Abstract The anti-tumour component (Fraction A) found in the culture filtrates of a local mushroom, Tricholoma sp. (strain STC20-T1), was purified using DEAE-cellulose ion exchange chromatography and Sepharose CL-4B gel filtration. Of its two purified sub-fractions, A-1 and A-2, only the latter showed anti-tumor activity. When Fraction A-2 was treated with cetyltrimethylammonium hydroxide and chloroform/butanol (24:1, v/v), it behaved as a proteinbound polysaccharide, with a molecular mass estimated to be 154 kDa by gel filtration. It contained 40% polysaccharide, which consisted of fucose, galactose, glucose, arabinose and mannose, and 30.5% protein, the amino-acid composition of which was determined. Fraction A-2 could inhibit the growth of Sarcoma-180 implanted in mice intraperitoneally and subcutaneously, with no sign of toxicity, at a dose of 20 mg/kg.day for 10 days.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1007/BF00286357
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