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  • 1
    Publikationsdatum: 2016-03-24
    Beschreibung: Dissolved organic matter (DOM) in the oceans is one of the largest pools of reduced carbon on Earth, comparable in size to the atmospheric CO2 reservoir. A vast number of compounds are present in DOM, and they play important roles in all major element cycles, contribute to the storage of...
    Print ISSN: 0027-8424
    Digitale ISSN: 1091-6490
    Thema: Biologie , Medizin , Allgemeine Naturwissenschaft
    Standort Signatur Erwartet Verfügbarkeit
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  • 2
    Publikationsdatum: 2016-09-10
    Beschreibung: In the naturally iron-fertilized surface waters of the northern Kerguelen Plateau region, the early spring diatom community composition and contribution to plankton carbon biomass were investigated and compared with the high nutrient, low chlorophyll (HNLC) surrounding waters. The large iron-induced blooms were dominated by small diatom species belonging to the genera Chaetoceros ( Hyalochaete ) and Thalassiosira , which rapidly responded to the onset of favorable light-conditions in the meander of the Polar Front. In comparison, the iron-limited HNLC area was typically characterized by autotrophic nanoeukaryote-dominated communities and by larger and more heavily silicified diatom species (e.g. Fragilariopsis spp.). Our results support the hypothesis that diatoms are valuable vectors of carbon export to depth in naturally iron-fertilized systems of the Southern Ocean. Furthermore, our results corroborate observations of the exported diatom assemblage from a sediment trap deployed in the iron-fertilized area, whereby the dominant Chaetoceros ( Hyalochaete ) cells were less efficiently exported than the less abundant, yet heavily silicified, cells of Thalassionema nitzschioides and Fragilariopsis kerguelensis . Our observations emphasize the strong influence of species-specific diatom cell properties combined with trophic interactions on matter export efficiency, and illustrate the tight link between the specific composition of phytoplankton communities and the biogeochemical properties characterizing the study area.
    Print ISSN: 0168-6496
    Digitale ISSN: 1574-6941
    Thema: Biologie
    Publiziert von Oxford University Press
    Standort Signatur Erwartet Verfügbarkeit
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  • 3
    Publikationsdatum: 2014-12-19
    Beschreibung: Iron availability in the Southern Ocean controls phytoplankton growth, community composition and the uptake of atmospheric CO2 by the biological pump. The KEOPS-2 experiment took place around the Kerguelen plateau in the Indian sector of the Southern Ocean, a region naturally fertilised with iron at the scale of hundreds to thousands of square kilometres, producing a mosaic of spring blooms which showed distinct biological and biogeochemical responses to fertilisation. This paper presents biogeochemical iron budgets (incorporating vertical and lateral supply, internal cycling, and sinks) for three contrasting sites: an upstream high-nutrient low-chlorophyll reference, over the plateau, and in the offshore plume east of Kerguelen Island. These budgets show that distinct regional environments driven by complex circulation and transport pathways are responsible for differences in the mode and strength of iron supply, with vertical supply dominant on the plateau and lateral supply dominant in the plume. Iron supply from "new" sources to surface waters of the plume was double that above the plateau and 20 times greater than at the reference site, whilst iron demand (measured by cellular uptake) in the plume was similar to the plateau but 40 times greater than the reference. "Recycled" iron supply by bacterial regeneration and zooplankton grazing was a relative minor component at all sites (
    Print ISSN: 1810-6277
    Digitale ISSN: 1810-6285
    Thema: Biologie , Geologie und Paläontologie
    Publiziert von Copernicus im Namen von European Geosciences Union.
    Standort Signatur Erwartet Verfügbarkeit
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  • 4
    Publikationsdatum: 2014-05-16
    Beschreibung: Microbial food web dynamics were determined during the onset of several spring phytoplankton blooms induced by natural iron fertilization off Kerguelen Island in the Southern Ocean (KEOPS2). The abundances of heterotrophic bacteria and heterotrophic nanoflagellates, bacterial heterotrophic production, bacterial respiration, and bacterial growth efficiency, were consistently higher in surface waters of the iron-fertilized sites than at the reference site in HNLC (high nutrient low chlorophyll) waters. The abundance of viral like particles remained unchanged, but viral production increased by a factor of 6 in iron-fertilized waters. Bacterial heterotrophic production was significantly related to heterotrophic nanoflagellate abundance and viral production across all sites, with bacterial production explaining about 70 and 85%, respectively, of the variance of each in the mixed layer (ML). Estimated rates of grazing and viral lysis, however, indicated that heterotrophic nanoflagellates accounted for a substantially higher loss of bacterial production (50%) than viruses (11%). Combining these results with rates of primary production and export determined for the study area, a budget for the flow of carbon through the microbial food web and higher levels during the early (KEOPS2) and the late phase (KEOPS1) of the Kerguelen bloom is provided.
    Print ISSN: 1810-6277
    Digitale ISSN: 1810-6285
    Thema: Biologie , Geologie und Paläontologie
    Publiziert von Copernicus im Namen von European Geosciences Union.
    Standort Signatur Erwartet Verfügbarkeit
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  • 5
    Publikationsdatum: 2014-10-24
    Beschreibung: Iron (Fe) uptake by the microbial community and the contribution of three different size-fractions was determined during spring phytoplankton blooms in the naturally Fe fertilized area off Kerguelen Islands (KEOPS2). Total Fe uptake in surface waters was on average 34 ± 6 pmol Fe L−1 d−1, and microplankton (〉25 μm size-fraction; 40–69%) and pico-nanoplankton (0.8–25 μm size-fraction; 29–59%) were the main contributors. The share of heterotrophic bacteria (0.2–0.8 μm size-fraction) to total Fe uptake was low at all stations (1–2%). Iron uptake rates normalized to carbon biomass were highest for pico-nanoplankton above the Kerguelen plateau and for microplankton in the downstream plume. We also investigated the potential competition between heterotrophic bacteria and phytoplankton for the access to Fe. Bacterial Fe uptake rates normalized to carbon biomass were highest when bacteria were incubated in the absence of both micro- and pico-nanoplankton. The absence of microplankton resulted in a decrease in bacterial Fe uptake rates by up to 20-fold, while in incubations with the whole microbial community bacterial uptake rates were reduced by 2- to 8-fold. In Fe-fertilized waters, the bacterial Fe uptake rates normalized to carbon biomass were positively correlated with primary production. Taken together, these results demonstrate that heterotrophic bacteria are outcompeted by small sized phytoplankton cells for the access to Fe during the spring bloom development, most likely due to the limitation by organic matter. We conclude that the Fe and carbon cycles are tightly coupled and driven by a~complex interplay of competition and synergy between different members of the microbial community.
    Print ISSN: 1810-6277
    Digitale ISSN: 1810-6285
    Thema: Biologie , Geologie und Paläontologie
    Publiziert von Copernicus im Namen von European Geosciences Union.
    Standort Signatur Erwartet Verfügbarkeit
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  • 6
    Publikationsdatum: 2014-10-02
    Beschreibung: Natural iron fertilization of high-nutrient low-chlorophyll (HNLC) waters induces annually occurring spring phytoplankton blooms off Kerguelen Islands (Southern Ocean). To examine the origin and fate of particulate and dissolved organic matter (POM and DOM), D- and L-amino acids (AA) were quantified at bloom and HNLC stations. Total hydrolysable AA accounted for 21–25% of surface particulate organic carbon (%POCAA) at the bloom sites, but for 10% at the HNLC site. A marked decrease in %POCAA with depth was observed at the most productive stations leading to values between 3 and 5% below 300 m depth. AA contributed to only 0.9–4.4% of dissolved organic carbon (%DOCAA) at all stations. The only consistent vertical trend was observed at the most productive station (A3-2) where %DOCAA decreased from ∼2% in the surface waters to 0.9% near 300 m. These AA yields and other markers revealed that POM and DOM were more rapidly altered or mineralized at the bloom sites compared to the HNLC site. Different molecular markers indicated that POM mostly originated from diatoms and bacteria. The estimated average proportion of POM from intact phytoplankton cells in surface waters was 45% at the bloom station A3-2, but 14% at the HNLC site. Estimates based on D-AA yields indicated that ∼15% of POM and ∼30% of DOM was of bacterial origin (cells and cell fragments) at all stations. Surprisingly, the DOM in HNLC waters appeared less altered than the DOM from the bloom, had slightly higher dissolved AA concentrations, and showed no sign of alteration within the water column. Unfavorable conditions for bacterial degradation in HNLC regions can explain these findings. In contrast, large inputs of labile organic molecules and iron, likely stimulate the degradation of organic matter (priming effect) and the production of more recalcitrant DOM (microbial carbon pump) during iron-fertilized blooms.
    Print ISSN: 1810-6277
    Digitale ISSN: 1810-6285
    Thema: Biologie , Geologie und Paläontologie
    Publiziert von Copernicus im Namen von European Geosciences Union.
    Standort Signatur Erwartet Verfügbarkeit
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  • 7
    Publikationsdatum: 2014-11-13
    Beschreibung: It has univocally been shown that iron (Fe) is the primary limiting nutrient for phytoplankton metabolism in High Nutrient Low Chlorophyll (HNLC) oceans, yet, the question of how this trace metal affects heterotrophic microbial activity is far less understood. We investigated the role of Fe for bacterial heterotrophic production and growth at three contrasting sites in the naturally Fe-fertilized region east of Kerguelen Islands and at one site in HNLC waters during the KEOPS2 (Kerguelen Ocean and Plateau Compared Study 2) cruise in spring 2011. We performed dark incubations of natural microbial communities amended either with iron (Fe, as FeCl3), or carbon (C, as trace-metal clean glucose), or a combination of both, and followed bacterial abundance and heterotrophic production for up to 7 days. Our results show that single and combined additions of Fe and C stimulated bulk and cell-specific bacterial production at all sites, while bacterial growth was enhanced only in two out of four occasions. The extent of stimulation of bulk bacterial heterotrophic production by single Fe or C additions increased with increasing in situ bacterial Fe uptake rates in the surface mixed layer. Our results provide evidence that both Fe and C are present at limiting concentrations for bacterial heterotrophic activity, in HNLC and fertilized regions, in spring. The observation that the extent of stimulation by both elements was related to Fe-uptake rates highlights the tight interaction between the C- and Fe-cycles through bacterial heterotrophic metabolism in the Southern Ocean.
    Print ISSN: 1810-6277
    Digitale ISSN: 1810-6285
    Thema: Biologie , Geologie und Paläontologie
    Publiziert von Copernicus im Namen von European Geosciences Union.
    Standort Signatur Erwartet Verfügbarkeit
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  • 8
    Publikationsdatum: 2012-12-21
    Beschreibung: In this study, we investigate the phytoplankton community response, with emphasis on ecophysiology and succession, after two experimental additions of Saharan dust in the surface layer of a low-nutrient low-chlorophyll ecosystem in the Mediterranean Sea. Three mesocosms were amended with evapocondensed dust to simulate realistic Saharan dust events while three additional mesocosms were kept unamended and served as controls. Experiments consisted in two consecutive dust additions and samples were daily collected at different depths (−0.1, −5 and −10 m) during one week, starting before each addition occurred. Data concerning HPLC pigment analysis on two size classes (〈 3 and 〉 3 µm), electron transport rate (ETR) versus irradiance curves, non-photochemical fluorescence quenching (NPQ) and phytoplankton cell abundance (measured by flow cytometry), are presented and discussed in this paper. Results show that picophytoplankton mainly respond to the first dust addition, while the second addition leads to an increase of both pico- and nano-/microphytoplankton. Ecophysiological changes in the phytoplankton community are revealed, and an increase in NPQ development, as well as in pigment concentration per cell, follows the dust additions. ETR does not show large variations between dust-amended and control conditions, while biomass increases in response to the dust additions. Furthermore, the biomass increase observed during this mesocosm experiment allows us to attempt a quantitative assessment and parameterization of the onset of a phytoplankton bloom in a nutrient-limited ecosystem. These results are discussed focusing on the adaptation of picophytoplankton to such a nutrient-limited mixed layer system, as well as on size-dependent competition ability in phytoplankton.
    Print ISSN: 1810-6277
    Digitale ISSN: 1810-6285
    Thema: Biologie , Geologie und Paläontologie
    Publiziert von Copernicus im Namen von European Geosciences Union.
    Standort Signatur Erwartet Verfügbarkeit
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  • 9
    Publikationsdatum: 2014-07-15
    Beschreibung: Above the Kerguelen Plateau in the Southern Ocean natural iron fertilization sustains a large phytoplankton bloom over three months during austral summer. During the KEOPS1 project (KErguelen Ocean and Plateau compared Study1) we sampled this phytoplankton bloom during its declining phase along with the surrounding HNLC waters to study the effect of natural iron fertilization on the role of viruses in the microbial food web. Bacterial and viral abundances were 1.7 and 2.1 times, respectively, higher within the bloom than in HNLC waters. Viral production and virus-mediated mortality of bacterioplankton was 4.1 and 4.9 times, respectively, higher in the bloom, while the fraction of infected cells (FIC) and the fraction of lysogenic cells (FLC) showed no significant differences between environments. The present study suggests viruses to be more important for bacterial mortality within the bloom and dominate over protozoan grazing during the late bloom phase. As a consequence, at least at a late bloom stage, viral lysis shunts part of the photosynthetically fixed carbon in iron-fertilized regions into the dissolved organic matter (DOM) pool with potentially less particulate organic carbon transfered to larger members of the food web or exported.
    Print ISSN: 1810-6277
    Digitale ISSN: 1810-6285
    Thema: Biologie , Geologie und Paläontologie
    Publiziert von Copernicus im Namen von European Geosciences Union.
    Standort Signatur Erwartet Verfügbarkeit
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  • 10
    Publikationsdatum: 2014-06-27
    Beschreibung: During KEOPS2 (Kerguelen Ocean and Plateau Compared Study 2), we determined dissolved inorganic and organic nitrogen and phosphorus species in the naturally fertilized region of Kerguelen Island (Southern Ocean). Above 150 m, stations were clearly separated by the Polar Front (PF), with concentrations of NO3–, NO2– and PO43– overall lower north than south of the PF. Though less pronounced, a similar trend was detectable for dissolved organic nitrogen (DON) and phosphorus (DOP). At all stations offshore and above the plateau, a subsurface maximum of NH4+ was observed between 50 and 150 m. We examined nutrient stoichiometry by calculating the linear combination N* = [NO3–] − 16[PO43– ]. The majority of stations and depths revealed N* close to −3 μM, however, for surface waters north of the PF N* increased up to 6 μM. This suggests a preferential uptake of PO43– vs. NO3– by fast growing diatoms. Using the tracer TNxs = [TDN] − 16[TDP] revealed that the dissolved organic fraction significantly contributed to changes in TNxs. TNxs were negative for most stations and depths, and relatively constant in the layer 0–500 m. As for N*, the stations north of the PF had higher TNxs in the layer 0–100 m. We discuss this stoichiometric anomaly with respect to possible external sources and sinks of N and P. Additional data collected in February 2013 at two sites revealed the occurrence of a subsurface of N* located just below the pycnocline that denotes a~layer where preferential remineralization of P vs. N persists throughout the season.
    Print ISSN: 1810-6277
    Digitale ISSN: 1810-6285
    Thema: Biologie , Geologie und Paläontologie
    Publiziert von Copernicus im Namen von European Geosciences Union.
    Standort Signatur Erwartet Verfügbarkeit
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