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  • 1
    Electronic Resource
    Electronic Resource
    Negative fallout from public sentiment in Japan (2004)
    [s.l.] : Nature Publishing Group
    Nature biotechnology 22 (2004), S. 943-943 
    Details
    ISSN: 1546-1696
    Source: Nature Archives 1869 - 2009
    Topics: Biology , Process Engineering, Biotechnology, Nutrition Technology
    Notes: [Auszug] To the editor: As concerned plant scientists at major plant science research institutions in Japan, we would like to express our collective concern over the impact of Japanese public resistance to plant genetic engineering on the actions of local and national government. We are concerned that ...
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1038/nbt0804-943a
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  • 2
    Electronic Resource
    Electronic Resource
    SECRETION OF OUTER MEMBRANE PROTEINS OF ESCHERICHIA COLI ACROSS THE CYTOPLASMIC MEMBRANE (1980)
    Oxford, UK : Blackwell Publishing Ltd
    Annals of the New York Academy of Sciences 343 (1980), S. 0 
    Details
    ISSN: 1749-6632
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Natural Sciences in General
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1111/j.1749-6632.1980.tb47265.x
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  • 3
    Electronic Resource
    Electronic Resource
    Distribution of the insertion sequence IS1 in Gram-negative bacteria (1981)
    [s.l.] : Nature Publishing Group
    Nature 289 (1981), S. 609-612 
    Details
    ISSN: 1476-4687
    Source: Nature Archives 1869 - 2009
    Topics: Biology , Chemistry and Pharmacology , Medicine , Natural Sciences in General , Physics
    Notes: [Auszug] Fig. 1 A, Map of IS1 , showing the locations of fragments used as hybridization probes. Italicized numbers indicate the coordinates of restriction endonuclease cutting sites, in nucleotides, from an end of ISI (see ref. 6). The Site as indicated. B, Physical structures of the resistance plasmid ...
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1038/289609a0
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  • 4
    Electronic Resource
    Electronic Resource
    Efficient transformation of Arabidopsis thaliana: comparison of the efficiencies with various organs, plant ecotypes and Agrobacterium strains (1992)
    Springer
    Plant cell reports 12 (1992), S. 7-11 
    Details
    ISSN: 1432-203X
    Keywords: Arabidopsis thaliana ; Transformation ; Agrobacterium ; T-DNA ; Shoot regeneration ; Transgenic plant
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary The efficiency of Agrobacterium-mediated transformation of Arabidopsis thaliana was compared with different organs, Arabidopsis ecotypes, and Agrobacterium strains. Efficiency of shoot regeneration was examined using hypocotyl, cotyledon and root explants prepared from young seedlings. Hypocotyl expiants had the highest regeneration efficiency in all of the four Arabidopsis ecotypes tested, when based on a tissue culture system of callus-inducing medium (CIM: Valvekens et al. 1988) and shoot-inducing medium (SIM: Feldmann and Marks 1986). Histochemical analysis using the ß-glucuronidase (GUS) reporter gene showed that the gusA gene expression increased as the period of preincubation on CIM was extended, suggesting that dividing cells are susceptible to Agrobacterium infection. In order to obtain transgenic shoots, hypocotyl explants preincubated for 7 or 8 days on CIM were infected with Agrobacterium containing a binary vector which carries two drug-resistant genes as selection markers, and transferred to SIM for selection of transformed shoots. Of four Arabidopsis ecotypes and of three Agrobacterium strains examined, Wassilewskija ecotype and EHA101 strain showed the highest efficiency of regeneration of transformed shoots. By combining the most efficient factors of preincubation period, Arabidopsis ecotype, tissue, and bacterial strain, we obtained a transformation efficiency of about 80–90%. Southern analysis of 124 transgenic plants showed that 44% had one copy of inserted T-DNA while the others had more than one copy.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00232413
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  • 5
    Electronic Resource
    Electronic Resource
    Genes coding for the major tuberous root protein of sweet potato: Identification of putative regulatory sequence in the 5′ upstream region (1988)
    Springer
    Plant molecular biology 11 (1988), S. 417-426 
    Details
    ISSN: 1573-5028
    Keywords: sweet potato ; tuberous roots ; sporamin ; storage protein ; genomic clone ; 5′ upstream region
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract The isolation and characterization is described of sporamin A and B genes, gSPO-A1 and gSPO-B1, respectively, which are members of two distinct subfamilies of the multigene family encoding the major soluble protein of sweet potato tuberous root. The nucleotide sequences of the two genes along with the 5′ and 3′ flanking regions were determined. The transcriptional start sites were determined by S1 nuclease mapping. Comparison of the sequences with those of the full-length cDNAs for sporamin A and B reveals that these genes contain no introns. Sequence comparison by dot matrix plot analysis shows that homology of the 5′ flanking regions between gSPO-A1 and gSPO-B1 extends only to 45 bp upstream of the transcription start site, which includes the consensus TATA box sequence. The sequence of this region is also conserved in the sporamin-related gene, gSPO-X1, which dose not seem to be expressed in the tuberous root. The sequences further upstream diverge extensively, but two short sequence blocks of 28 to 29 bp and 19 to 22 bp are found in the 5′ flanking sequence of gSPO-A1 and gSPO-B1 but not in gSPO-X1. The 19–22 bp sequence block is repeated twice in gSPO-A1 and four times in gSPO-B1. These conserved sequence blocks may play regulatory roles in their expression.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00039022
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  • 6
    Electronic Resource
    Electronic Resource
    Isolation and characterization of two tightly linked catalase genes from castor bean that are differentially regulated (1994)
    Springer
    Plant molecular biology 25 (1994), S. 507-516 
    Details
    ISSN: 1573-5028
    Keywords: castor bean (Ricinus communis) ; catalase gene ; gene expression ; germination
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Two catalase genes,cat1 andcat2, have been isolated from the castor bean genome. They were located in the same direction on a chromosome at a distance of 2.4 kb,cat1 being on the downstream side ofcat2. The two genes contained introns at the same positions except that one of the 7 introns incat1 is missing incat2 and the corresponding introns differed in size and sequence between the two genes. The translated regions of the two genes had the same number of nucleotides and exhibited 81.3% nucleotide sequence identity. In addition to introns, the nucleotide sequences of the 5′-and 3′-flanking regions are highly divergent between the two genes. In etiolated seedlings,cat1 mRNA was present abundantly in endosperms and cotyledons and only in a small amount in roots. Thecat1 mRNA could not be detected in hypocotyls. By contrast,cat2 mRNA is most abundant in hypocotyls and roots, while endosperms and cotyledons contained only low levels ofcat2 mRNA. Although neithercat1 norcat2 mRNA could be detected in dry seeds, both mRNAs showed temporal accumulation in the endosperm in response to germination. These results suggest that expression of two tightly linked catalase genes of castor bean,cat1 andcat2, are differentially regulated during development.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00043878
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  • 7
    Electronic Resource
    Electronic Resource
    Structural relationship among the members of a multigene family coding for the sweet potato tuberous root storage protein (1989)
    Springer
    Plant molecular biology 13 (1989), S. 563-572 
    Details
    ISSN: 1573-5028
    Keywords: multigene family ; sweet potato ; Ipomoea batatas ; storage protein ; sporamin ; evolution
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Sporamin, the major soluble protein of the sweet potato tuberous root, is coded for by a multigene family. Fourty-nine essentially full-length sporamin cDNAs isolated from tuberous root cDNA library have been classified by cross hybridization, restriction endonuclease cleavage pattern and ribonuclease cleavage mapping. All the cDNAs fall into one of the two distinct homology groups, subfamilies A and B, which correspond to the polypeptide classes sporamin A and B, respectively. At least 5 different sequences are detected in both of the 22 sporamin A and 27 sporamin B cDNAs. Comparison of the nucleotide sequences of the coding region of three each of sporamin A and B subfamily members, four from cDNAs and two from genomic clones, indicates that intra-subfamily homologies (94 to 98%) are much higher than inter-subfamily homologies (82 to 84%), and there are deletions or insertions of one or two codons at three locations which characterize each subfamily. Large portions of base substitutions in the coding region accompany amino acid substitutions. In contrast to the coding region, most of the structural differences among the members in the 5′ and 3′ noncoding regions are deletions or insertions.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00027316
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  • 8
    Electronic Resource
    Electronic Resource
    The nuclear factor SP8BF binds to the 5′-upstream regions of three different genes coding for major proteins of sweet potato tuberous roots (1992)
    Springer
    Plant molecular biology 18 (1992), S. 97-108 
    Details
    ISSN: 1573-5028
    Keywords: β-amylase gene ; sequence-specific DNA-binding protein ; SP8BF ; sporamin gene ; sweet potato
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Sporamin and β-amylase are two major proteins of tuberous roots of sweet potato, and expression of genes coding for sporamin and β-amylase is induced concomitantly in leaves with the petioles attached by exogenous supply of sucrose or polygalacturonic acid. We have used a DNase I footprinting assay to characterize nuclear factors that bind to the 5′-upstream regions of gSPO-A1, gSPO-B1 and gβ-Amy genes that encode A-type sporamin, B-type sporamin and the subunit of β-amylase, respectively. Nuclear extracts from sucrose-treated petioles protected a region around -155 relative to the transcription start site of gSPO-A1 and a region around -880 of gβ-Amy from DNase I digestion on both strands. These two protected regions both contained the sequence ACTGTGTA, designated SP8a, in opposite orientation with respect to the direction of transcription. A gel mobility shift assay with SP8a oligonucleotide and competition experiments indicated that a common factor SP8BF binds to the SP8a sequence in gSPO-A1 and gβ-Amy. Binding of SP8BF to the SP8a oligonucleotide was abolished by mutation within the SP8a sequence. Fragments of the 5′-upstream region of gSPO-B1 also competed for the binding of SP8BF to the SP8a oligonucleotide, and the DNase I footprinting assay revealed three binding sites for SP8BF in the 5′-upstream region of gSPO-B1. These three sites in gSPO-B1 all contained the sequence TACTATT, designated SP8b, which shared 4 nucleotides at identical positions with the SP8a sequence. An inverted repeat of the SP8b sequence was also present at one protected site in the 5′-upstream region of gβ-Amy. In addition to sucrose-treated petioles, SP8BF activity was also present in tuberous roots and untreated fresh petioles of sweet potato. Furthermore, the activity was also detected in stems of tobacco plantlets, suggesting that SP8BF is an ubiquitous factor.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00018460
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  • 9
    Electronic Resource
    Electronic Resource
    Jasmonate-inducible expression of a potato cathepsin D inhibitor-GUS gene fusion in tobacco cells (1994)
    Springer
    Plant molecular biology 26 (1994), S. 403-414 
    Details
    ISSN: 1573-5028
    Keywords: cathepsin D inhibitor ; methyl jasmonate ; promoter analysis ; transformed tobacco cells ; tuberonic acid
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract A potato gene encoding cathepsin D inhibitor (CDI) is expressed constitutively in tubers and flower buds and it is inducible in leaves upon wounding of the tissue or by treatment with methyl jasmonate (MJA). A fusion gene (CDI:GUS) in which the 2.4 kb long promoter of the CDI gene was translationaly fused with the coding sequence for β-glucuronidase (GUS) showed MJA-inducible expression in transformed tobacco cells in suspension. The maximum level of induction by MJA was obtained in the absence of auxin and repression of MJA-inducible expression of the fusion gene by auxin was released by aphidicolin, the results suggesting that MJA-inducible expression is repressed during active cell division. JA and MJA showed similar activities in inducing the expression of the fusion gene, while other JA-related compounds such as cucurbic acid, tuberonic acid and dihydrojasmonic acid neither induced expression of the fusion gene nor inhibited the MJA-inducible expression of the fusion gene. Methyl dihydrojasmonate specifically stimulated the MJA-inducible expression of the fusion gene. The MJA-inducible expression of the fusion gene was observed even with a 100 bp long promoter of the CDI gene albeit with significantly decreased level of expression compared to the 2.4 kb long promoter. The 100 bp long CDI promoter did not contain a G-box or hexamer motif that had been implicated in the MJA-responsive expression of several other plant genes. Further mutagenesis of the 100 bp long promoter by deletion or oligonucleotide insertion suggested that although a sequence between −100 and −82 is required for the MJA-responsive expression, the presence of this sequence alone does not confer the MJA-responsive expression.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00039549
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  • 10
    Electronic Resource
    Electronic Resource
    High-level expression of tuberous root storage protein genes of sweet potato in stems of plantlets grown in vitro on sucrose medium (1990)
    Springer
    Plant molecular biology 14 (1990), S. 595-604 
    Details
    ISSN: 1573-5028
    Keywords: tuber storage protein ; sporamin ; sucrose ; sweet potato ; Ipomoea batatas
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Sporamin, the tuberous root storage protein of the sweet potato, accounts for about 60 to 80% of the total soluble protein of this organ. The amount of sporamin present in other organs is very low, or even not detectable, in the normal field-grown plants. However, the stem of sweet potato plantlets grown axenically on agar medium containing sucrose was found to accumulate large amounts of sporamin. Two-dimensional gel electrophoretic profiles of sporamin precursors synthesized in vitro by poly(A)+ RNA are indistinguishable between tuberous roots of the field-grown plants and stems of the axenically cultured plants, suggesting that an essentially identical set of the members of sporamin multigene family are expressed in these two organs under different growth conditions. Transgenic tobacco plants having a CAT (chloramphenicol acetyltransferase) fusion gene with the 5′ upstream region of a sporamin A gene, gSPO-A1, show preferential expression of CAT activity in stems when the plants are maintained in axenic culture on sucrose medium as is the case for sporamin in sweet potato. Deletion analysis revealed that the DNA sequence of gSPO-A1 between −94 and −305, relative to the transcription start site, is important for its expression in tobacco. This region contains two of the previously postulated putative regulatory elements conserved between sporamin A and B genes.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00027505
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