ISSN:
1432-203X
Keywords:
Key wordsActinidia eriantha
;
Chromosome number
;
Multinucleate cells
;
Plant regeneration
;
Protoplast culture
Source:
Springer Online Journal Archives 1860-2000
Topics:
Biology
Notes:
Abstract Newly expanded in vitro leaves of Actinidia eriantha were used for protoplast isolation. Protoplasts were cultured in liquid MS medium (lacking NH4NO3) supplemented with 2,4-D (2,4-dichlorophenoxyacetic acid) and 0.4 M glucose. The plating efficiency after 3 weeks of culture was 19.4%, and calli were recovered without addition of fresh medium. These calli regenerated shoots on transfer to MS medium containing 2.28 μM zeatin and 0.57 μM IAA (indole-3-acetic acid). Regenerated shoots were rooted by immersion in 20 ppm IBA (indole-3-butyric acid) solution before culturing on half-strength MS medium lacking growth regulators. Somaclonal variation, in terms of chromosome number and nuclei per cell of protoplast-derived plants, was estimated.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1007/s002990050490
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