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  • 1
    Electronic Resource
    Electronic Resource
    Recovery and Purification of Soap Lye Glycerine by the Garrigues Process. (1910)
    s.l. : American Chemical Society
    Industrial & engineering chemistry 2 (1910), S. 283-293 
    Details
    ISSN: 1520-5045
    Source: ACS Legacy Archives
    Topics: Chemistry and Pharmacology , Process Engineering, Biotechnology, Nutrition Technology
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1021/ie50018a016
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  • 2
    Electronic Resource
    Electronic Resource
    Production of pineapple plants in vitro (1988)
    Springer
    Plant cell reports 7 (1988), S. 535-537 
    Details
    ISSN: 1432-203X
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract In vitro culture of pineapple (Ananas comosus) was studied to determine the efficiency of axillary bud culture for rapid propagation of several cultivars. The technique used maximizes the success rate of various steps in the production of pineapple plants. Rapid mass multiplication of plantlets started 9 months after explanting with a significant log phase. The number of plantlets obtained from the culture of a single bud by the thirteenth month ranged from 210 to 380 for ‘Perolera’; 300 to 350 for ‘PR-1-67’; and 40 to 85 for ‘Smooth Cayenne’. The method permits culture of a range of pineapple cultivars. Little morphological variation was observed in young regenerated plants.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00272750
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  • 3
    Electronic Resource
    Electronic Resource
    Agrobacterium-mediated transformation of Citrus stem segments and regeneration of transgenic plants (1992)
    Springer
    Plant cell reports 11 (1992), S. 238-242 
    Details
    ISSN: 1432-203X
    Keywords: Carrizo citrange ; Key lime (C. aurantifolia) ; Genetic transformation
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary A method for Agrobacterium-mediated transformation of Citrus and organogenic regeneration of transgenic plants is reported. Internodal stem segments were co-cultured with Agrobacterium harboring binary vectors that contained the genes for the scorable marker ß-glucuronidase (GUS) and the selectable marker NPT-II. A low but significant percentage (≤ 5%) of the shoots regenerated in the presence of 100 μg/ml kanamycin were GUS+. Polymerase chain reaction (PCR) analysis confirmed that GUS+ shoots contained T-DNA. Two plants established in soil were shown to be transgenic by Southern analysis.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00235073
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  • 4
    Electronic Resource
    Electronic Resource
    Agrobacterium-mediated transformation of peanut (Arachis hypogaea L.) embryo axes and the development of transgenic plants (1995)
    Springer
    Plant cell reports 14 (1995), S. 699-703 
    Details
    ISSN: 1432-203X
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Transgenic peanut (Arachis hypogaea L.) plants have been produced using an Agrobacterium-mediated transformation system. Zygotic embryo axes from mature seed were cocultured with Agrobacterium tumefaciens strain EHA101 harboring a binary vector that contained the genes for the scorable marker B-glucuronidase (GUS) and the selectable marker neomycin phosphotransferase II. Nine percent of the germinated seedlings were GUS+. Polymerase chain reaction analysis confirmed that GUS+ shoots and T1 progeny contained T-DNA. Molecular characterization of one primary transformant and its T1 and T2 progeny plants established that T-DNA was integrated into the host genome.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00232650
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  • 5
    Electronic Resource
    Electronic Resource
    Factors affecting Agrobacterium-mediated transformation in Citrus and production of sour orange (Citrus aurantium L.) plants expressing the coat protein gene of citrus tristeza virus (1997)
    Springer
    Plant cell reports 16 (1997), S. 745-753 
    Details
    ISSN: 1432-203X
    Keywords: Key words Genetic transformation ; Key lime ; Carrizo citrange
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Factors influencing transformation frequencies using the Agrobacterium-mediated protocol developed for Citrus seedling internodal stem segments in this laboratory were evaluated, with particular emphasis on decreasing the numbers of ``escape'' shoots produced. Although the use of a wild-type ``shooty'' Agrobacterium strain allowed relatively high frequencies of β-glucuronidase positive (GUS+) shoots to be produced, none of the shoots were free of wild-type T-DNA and would not root. Both use of a liquid medium/kanamycin overlay and horizontal placement of stem segments increased the efficiency of kanamycin selection. Wounding via particle bombardment prior to Agrobacterium inoculation did not increase transformation frequencies. The concentration of benzyladenine (BA) in the regeneration/selection medium inversely influenced the numbers of shoots that regenerated and the subsequent ability of the shoots to root. Regeneration in the presence of kanamycin also influenced the ability of shoots to root. Many of the shoots that regenerated on selection medium were chimeric for GUS expression, and plants established from such shoots ranged from non-staining to solidly staining for GUS. However, solidly transformed plants with integrated T-DNA were obtained, and these plants have maintained the expression of transgenes over several years. The transgenic plants include ones of sour orange (C. aurantium L.) and Key lime (C. aurantifolia (Christm.) Swing.), two species not previously transformed, and have integrated and express the coat protein gene of citrus tristeza virus. This is the first report of a potentially agriculturally important transgene being expressed in Citrus.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/s002990050313
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  • 6
    Electronic Resource
    Electronic Resource
    Variations in response to induced stem bending in seedlings of Pinus radiata (1994)
    Springer
    Trees 8 (1994), S. 151-159 
    Details
    ISSN: 1432-2285
    Keywords: Pinus radiata ; Stem deformation ; Nitrogen, elasticity ; Compression wood ; Stem form
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Summary The occurrence of stem deformation in Pinus radiata plantations has been examined previously in terms of the factors leading to the onset of bending symptoms. In this study the influence of seedling family and nitrogen availability on the ability of seedlings to recover from deformation was investigated under glasshouse conditions. Seedlings from four families ranging from resistant to susceptible to stem deformation were used. Stem deformation was induced by loading stems with a known weight and comparing bending moments and rates of recovery. Significant differences between families were found with more susceptible families exhibiting greater initial magnitudes of stem lean. Similarly these families also exhibited greater rates of apical elongation following bending despite the larger stem leans. This resulted in more exaggerated stem bends as a consequence of the recovery of the lower stem pushing the upper stem away from the vertical. The strength characteristics of the stems could not explain differences between families. Susceptibility to stem deformation was explained more by stem slenderness expressed as stem height to root collar diameter.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00196639
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  • 7
    Electronic Resource
    Electronic Resource
    Cloning and characterization of NBS-LRR class resistance-gene candidate sequences in citrus (2000)
    Springer
    Theoretical and applied genetics 101 (2000), S. 814-822 
    Details
    ISSN: 1432-2242
    Keywords: Key words Disease resistance genes ; Citrus tristeza virus resistance ; Citrus nematode resistance ; Molecular markers ; Genetic mapping
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract  Numerous disease resistance gene-like DNA sequences were cloned from an intergeneric hybrid of Poncirus and Citrus, using a PCR approach with degenerate primers designed from conserved NBS (nucleotide-binding site) motifs found in a number of plant resistance genes. Most of the cloned genomic sequences could be translated into polypeptides without stop codons, and the sequences contained the characteristic motifs found in the NBS-LRR class of plant disease resistance genes. Pairwise comparisons of these polypeptide sequences indicated that they shared various degrees of amino-acid identity and could be grouped into ten classes (RGC1–RGC10). When the sequences of each class were compared with known resistance-gene sequences, the percentage of amino-acid identity ranged from 18.6% to 48%. To facilitate genetic mapping of these sequences and to assess their potential linkage relationship with disease resistance genes in Poncirus, we developed CAPS markers by designing specific primers based on the cloned DNA sequences and subsequently identifying restriction enzymes that revealed genetic polymorphisms. Three of the amplified DNA fragment markers (designated as 18P33a, Pt9a, and Pt8a) were associated with the citrus tristeza virus resistance gene (Ctv), and one fragment (Pt8a) was associated with the major gene responsible for the citrus nematode resistance (Tyr1); both genes are from Poncirus and of importance to citrus survival and production. These polymorphic fragments were located on two local genetic linkage maps of the chromosome region from Ctv to Tyr1. These results indicate that resistance-gene candidate sequences amplified with the NBS-derived degenerate primers are valuable sources for developing markers in disease resistance-gene tagging, mapping, and cloning.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/s001220051548
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  • 8
    Electronic Resource
    Electronic Resource
    The use of leaf GPI and IDH isozymes to examine the origin of polyembryony in cultured ovules of seedless grape (1989)
    Springer
    Plant cell reports 7 (1989), S. 669-672 
    Details
    ISSN: 1432-203X
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Isozyme analysis of young leaf tissue was performed on progeny produced from cultured ovules resulting from crosses of seedless × seedless grapes. Two polymorphic loci, Idh and Gpi-c, were found to segregate in a simple Mendelian fashion in the populations examined. Analyses of 70 seedlings indicated that most seedlings produced by this method were zygotic. Isozyme data of 11 plants from 5 polyembryonic ovules, along with data previously reported, suggest that several mechanisms may be operative in grape that give rise to polyembryony: 1) fertilization and development of more than one cell in the embryo sac, 2) adventive embryogenesis from the zygote, or 3) embryogenic development of gametic cells in addition to the zygote in the embryo sac.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00272057
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  • 9
    Electronic Resource
    Electronic Resource
    Linkage of restriction fragment length polymorphisms and isozymes in Citrus (1992)
    Springer
    Theoretical and applied genetics 84 (1992), S. 39-48 
    Details
    ISSN: 1432-2242
    Keywords: Genetic map ; Molecular markers ; RFLP ; Fruit breeding ; Citrus spp.
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary Genetic linkage analysis was performed using two segregating populations of citrus. One population arose from an intergeneric backcross of Citrus grandis (L.) Osb. cv ‘Thong Dee’ and Poncirus trifoliata (L.) Raf. cv ‘Pomeroy’, using the former as the recurrent (female) parent. The other population came from an interspecific backcross of C. reticulata Blanco cv ‘Clementine’ and C. x paradisi Macf. cv ‘Duncan’, using the former as the recurrent (male) parent. A total of 11 isozyme and 58 restriction fragment length polymorphisms were found to segregate in a monogenic fashion in one or both populations. Linkage analysis revealed that 62 of the loci examined mapped to 11 linkage groups, while 7 loci segregated independently from all other markers. Gene order was highly conserved between the maps generated from the two divergent segregating populations. Possible applications of the use of such maps in tree fruit breeding are discussed.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00223979
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  • 10
    Electronic Resource
    Electronic Resource
    Extension of the linkage map in Citrus using random amplified polymorphic DNA (RAPD) markers and RFLP mapping of cold-acclimation-responsive loci (1994)
    Springer
    Theoretical and applied genetics 89 (1994), S. 606-614 
    Details
    ISSN: 1432-2242
    Keywords: Genetic map ; RAPD, RFLP ; Molecular markers ; Fruit breeding
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Genetic mapping with RAPD markers has been initiated in Citrus. Reproducible polymorphism of amplified DNA fragments was obtained with approximately half of the 140 random primers tested, revealing 266 segregating loci. These were tested for linkage using 60 BC1 progeny from an intergeneric cross of Citrus grandis (L.) Osb. x [Citrus grandis (L.) Osb. x Poncirus trifoliata (L.) Raf.]. A core linkage map was constructed that consists of nine linkage groups containing 109 RAPD markers and 51 previously-mapped RFLP and isozyme markers. A further 79 markers that could not be ordered unambiguously because of their allelic constitution were associated with individual linkage groups and are shown in relation to the core map. The core map has a total length of 1192 cM with an average distance of 7.5 cM between loci and is estimated to cover 70–80% of the genome. Loci with distorted segregation patterns clustered on several linkage groups. Individual clusters of loci were skewed in allelic composition toward one or the other parent, usually C. grandis. This relatively-saturated linkage map will eventually be used to identify quantitative trait loci for cold and salt-tolerance in Citrus. As a beginning we have mapped three loci detected by a cold-acclimation-responsive cDNA.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00222455
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