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  • 1
    Publication Date: 2014-02-25
    Print ISSN: 1863-0650
    Electronic ISSN: 1863-0669
    Topics: Energy, Environment Protection, Nuclear Power Engineering
    Published by Wiley
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  • 2
    Publication Date: 2021-05-19
    Description: Protection authorities of plant and animal genetic resources and conservation of biodiversity as national assets by using biotechnology techniques is the most important objectives of genetic data information bank. Establishment of shrimp species genetic data bank, makes possibility of study multiple genes such as economic genes and since knowledge of genetic variation within and among populations and barcoding species of shrimp are very important for species conservation and with so little researches of Asia in the global studies of DNA barcoding and animal taxonomy in Iran, In this study, Iran shrimp genetic diversity and genetic data bank was done. DNA barcode is a short, standard well known sequence of cytochrome oxidase І gene. By using this DNA sequence can be realized that each animal, plant or fungus belongs to which species. Also prepare a bank of genetic data shrimp pathogens and maintenance of these strains are steps to advance future researches in the fields of pathogenesis mechanism of pathogens, diagnosis, treatment, Disease prevention, production diagnosis native kits and detection of emerging and reemerging diseases its origins. So in this plan samples were collected from imported cultured shrimp Litopenaeus vannamei and and 6 Persian Gulf and Oman Sea shrimp species which classified based on traditional systematically as: Penaeus semisulcatus, Fenneropenaeus merguiensis, Metapenaeus affinis, Parapenaeopsis Stylifera and Fenneropenaeus indicus. After examination of DNA barcode sequence, molecular and bioinformatics operations of each sequence in the Consortium for the Barcode of Life (CBOL) and National Center for Biotechnology Information (NCBI), phylogenetic analysis of each sample was determined and similarity of each sample with NCBI and CBOL database was checked and the closest species to each sample were specified. According to the results different samples of L. vannamei, . banded P.semisulcatus, F. merguiensis and F. indicus have more than 97% similarity to the same species of other countries. non banded P.semisulcatus had 80.07% similarity to banded P.semisulcatus, M. affinis samples had 90.3% similarity to Metapenaeus ensis and Parap. Stylifera had 93.44% similarity to Parapenaeopsis coromandelica in the CBOL. This funding confirmed the need for further investigation and possible announcement of new species. By using ribotyping technique, native isolated pathogenic bacteria and fungi were identified and recorded in the gene bank database center. During sampling of shrimp and water of Specific Pathogen Free shrimp center, 40 bacterial strains were isolated, which 8 of them had the most frequency and identification based on 16S rDNA sequencing was performed. Bacteria identified are: Vibrio nigripulchritudo strain IS013(GenBank:KP843725) ،Vibrio brasiliensis strain IS014 (GenBank:KR186076) ،Vibrio rotiferianus strain IS015 (GenBank:KR186077) ،Vibrio azureus strain IS012 (GenBank:KJ018724.1) ،Vibrio owensii strain IS016 (GenBank:KR186078) ،Agarivorans gilvus strain IS017 (GenBank:KR186079) ،Vibrio brasiliensis IS018 (GenBank:KR186080) and Vibrio alginolyticus strain IS019 (GenBank:1817854), which were recorded in The World Bank genes. In this study fungal isolates were not detected.
    Description: Iranian Fisheries Science Research Institute
    Description: Published
    Keywords: Bacteria ; Ribotyping ; 16S rDNA ; Shrimp ; DNA barcode ; GenBank ; Vibrio nigripulchritudo ; Vibrio rotiferianus ; Vibrio owensii ; Vibrio brasiliensis ; Vibrio azureus ; Agarivorans gilvus ; Vibrio alginolyticus ; Parapenaeopsis coromandelica ; Metapenaeus ensis ; Litopenaeus vannamei
    Repository Name: AquaDocs
    Type: Report , Refereed
    Format: 114pp.
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  • 3
    Publication Date: 2021-05-19
    Description: At present, the aquaculture industry to provide proper instructions in the field of health management, including production of Specific Pathogen Free shrimp (SPF), require sensitive and reliable methods for the detection and identification of pathogenic microorganisms. Molecular methods which used in the detection of microorganisms have a high discriminatory power in the taxonomy and in relation to libraries in the world. On the other hand, the accurate identification of microorganisms, providing the genetic data bank of shrimp pathogens and maintenance of these strains is the step to promote further research on the mechanisms of pathogenesis of pathogens, diagnosis, treatment, prevention of disease, identify indigenous production kits, diagnosis re emerging and emerging diseases and their origin. Therefore, in this project, by using ribotyping technique, native isolated pathogenic bacteria and fungi were identified and recorded in the gene bank database center. During sampling of shrimp and water of Specific Pathogen Free shrimp center, 40 bacterial strains were isolated, which 8 of them had the most frequency and identification based on 16S rDNA sequencing was performed. Bacteria identified are: Vibrio nigripulchritudo strain IS013(GenBank:KP843725), Vibrio brasiliensis strain IS014 (GenBank:KR186076), Vibrio rotiferianus strain IS015 (GenBank:KR186077), Vibrio azureus strain IS012 (GenBank:KJ018724.1), Vibrio owensii strain IS016 (GenBank:KR186078), Agarivorans gilvus strain IS017 (GenBank:KR186079), Vibrio brasiliensis IS018 (GenBank:KR186080) and Vibrio alginolyticus strain IS019 (GenBank:1817854), which were recorded in The World Bank genes. In this study fungal isolates were not detected.
    Description: Iranian Fisheries Science Research Institute
    Description: Published
    Keywords: Bacteria ; Fungi ; Ribotyping ; 16S rDNA ; Shrimp ; Specific pathogen free ; Vibrio rotiferianus ; Vibrio owensii ; Vibrio brasiliensis ; Vibrio azureus ; Agarivorans gilvus ; Vibrio alginolyticus ; GenBank
    Repository Name: AquaDocs
    Type: Report , Refereed
    Format: 64pp.
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  • 4
    Publication Date: 2021-05-19
    Description: In order to continue improving the quality of Iranian fisheries science research institute's laboratories activities, For the first time in the years 2014-2015, a project with the subject of ―risk assessment of laboratories‖ conducted in Iran Shrimp Research Center, to determine the risk factors, potential risks and provide action plans to prevent the occurrence of any risks and increasing awareness of staff to safety issues. This project was done in eight laboratories which were named: Plankton, Pathology, Molecular genetics (extraction and elechtrophoresis), Pollutants, Marine physical chemistry, Sediment and benthos, Aquatic animal biometry and Microbiology with Failure Mode and Effects Analysis (FMEA) methods. The assessment process was designed and implemented in two phases: at the first phase, the current situation of safety indexes, risks identification and distance to laboratory safety parameters included: working environment, physical factors, test methods and the human factors were done, then risk assessment and risk management of identified risks were followed by check list forms. At the second phase, based on the findings ,control measures and safety guidelines were done and provided. According to the findings, the highest risk score related to Microbiology laboratory and pollutant laboratory with RPN 210, at the next level Marine physical chemistry laboratory with risk number 150 and respectively Molecular genetics laboratory (extraction), Sediment and benthos, Pathology, Molecular genetics (electrophoresis), Plankton and Aquatic animal biometry laboratories were existed. After control measures, the risk number of laboratories decreased as follows: Microbiology to 150, Pollutants to 120, Marine physical chemistry laboratory to 80, Molecular genetics (extraction) and Sediment and Benthos Laboratories to 48, Pathology decrease to 18, Molecular genetics (electrophoresis), Plankton and Aquatic animal biometry laboratories decreased to 8. It is hoped that by promoting safety knowledge of laboratory staff and providing financial can be able to reduce risk assessment number of all laboratories to very low rank.
    Description: Iranian Fisheries Science Research Institute
    Description: Published
    Keywords: Assessment ; Risk ; FMEA ; Laboratory ; Marine physical chemistry laboratory ; Molecular genetics ; Sediment and Benthos Laboratories
    Repository Name: AquaDocs
    Type: Report , Refereed
    Format: 84pp.
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  • 5
    Publication Date: 2021-05-19
    Description: Shrimp culture and rearing industry in particular white leg shrimp species (Litopenaeus vannamei) is one of the major activities in the world, including Iran. The outbreaks of diseases are the main factors limiting production. Due to the adverse effects of abusing antibiotics and chemical disinfectants, disease control and prevention requires novel methods that are affordable, effective and safe for the environment and humans. For this purpose during the five months, sampling was done from three main sites of shrimp culture in Bushehr province and 150 pieces cultured white leg shrimp, 135 water and sediment samples were collected from the ponds, input and output channels by standard methods. Physicochemical parameters of water and biometry of shrimps were done and recorded. Probiotic isolation was performed by culturing samples in Tryptic soy agar and TCBS after incubation in 30º C for 24-48 h. The antimicrobial effects of culture extract of isolates were evaluated against V. harveyi with the well diffusion method and the best isolates were selected. The molecular identification of selected bacteria was performed by 16S ribosomal DNA gene sequence analysis technique. The growth kinetics of selected bacteria and effect of environmental factors on antimicrobial compound production and stability of them in salinity (1.5-5.5%) and (0-50ppt), pH (5-9) and temperature (3040º C) and (35-100º C) were studied respectively. After the determination of antibiotic resistance profiles of them, the In vivo test on the L. vannamei post larvae was performed and effect of these bacteria on growth performance, prevention of vibriosis and water quality include (ammonia, nitrate nitrite, turbidity, total bacteria and Vibrionaceae load were examined. For extraction and identification of bioactive material with anti-bacterial properties produced by selected bacteria, dialysis, SDS-PAGE electrophoresis, ultraviolet spectroscopy and gas chromatography mass was performed. According to the results of this dissertation the most frequency of facultative aerobic and anaerobic heterotrophic bacteria was seen in shrimp digestion tract (3.04 ± 0.75) ×105 CFU/g in September .Genus Vibrio spp. (37.88%) and Bacillus spp. (27.27%) had the most frequency respectively. Of the 198 bacterial isolates, two bacterial strains from the digestive tract and sediment which had the highest inhibition and a stability antagonistic effect on V. harveyi were selected. Based on 16S ribosomal DNA gene sequence analysis, they were identified as Bacillus subtilis subsp. inaquosorum strain IS02 (GenBank: JN856456.1) and Bacillus vallismotis IS03 (GenBank: JQ085958.1) and recorded in the National Center for Biotechnology Information (NCBI). According to results of identifying bioactive material produced by selected bacteria, in SDS-PAGE electrophoresis Bacillus subtilis subsp. inaquosorum strain IS02 did not have any band but Bacillus vallismotis IS03 had two band in 25 kDa and 34-45 kDa molecular weight which probably bacteriocin like substances and group III Bacillus bacteriocin respectively. UV spectra of selected bacteria were in the range (260-265nm) which related to peptide groups. In GC-MS the highest percent of chromatogram was belonged to pyrrolopyrazines in both of selected bacteria, which have antibacterial properties. Overall, according to results both of the selected bacteria were adapted to the ecological conditions of shrimp culture and could be used as appropriate probiotics in this industry. It is hoped that the results of this research is to produce a step towards the realization of national production of indigenous probiotic in Iran.
    Description: Iranian Fisheries Science Research Institute
    Description: Published
    Keywords: Chemical ; Environmental ; Isolation ; Molecular ; Probiotic Bacteria ; White leg Shrimps ; Litopanaeus vannamei ; Shrimp ; Culture ; Species ; Sediment ; L. vannamei ; Genus Vibrio spp. ; Bacillus spp. ; V. harveyi
    Repository Name: AquaDocs
    Type: Report , Refereed
    Format: 237pp.
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  • 6
    Publication Date: 2021-05-19
    Description: Aquaculture is the fastest growing food industry in the world. Shrimp culture industry is also part of it, unfortunately, like other marine animal culture economic losses caused by the disease has been one of the major challenges of this industry. The major cause of mortality in shrimp hatcheries and rearing centers is related to water quality and the presence of pathogenic bacteria and parasites. These are common opportunistic microorganisms in the hatchery, rearing centers, flora and living food but poor conditions of culture are caused diseases. Since the development of aquaculture in the countries need health management, one of the important additional rings in the shrimp strategic plan is specific pathogen free shrimp production, which has been addressed in this plan. Specific pathogen free shrimp define as the shrimps which are free of the specific pathogens listed in world organization for animal health (OIE). These factors should be conclusively diagnosed and can be isolated from shrimp hatcheries and culture system. Therefore in this project screening and surveillance of shrimp in several generations according to the list of OIE were done and they were monitored for of bacterial pathogens (Necrosis Hepatopancreas Bacteria) and parasites (Microsporidian and Gregarins). At total 756 pieces of shrimp, 6 sample of dry food and 97 samples of live foods were controlled and tested. 1.35 percent of live foods were positive for NHPB and 5.6 percent of pre broodstocks have eppicommensal and microsporidia which were disposed in quarantine phase. Because of biosecurity and surveillance system establishment, there were no bacterial or parasitic isolation or diagnosis during SPF shrimp production.
    Description: Iranian Fisheries Science Research Institute
    Description: Published
    Keywords: Litopenaeus vannamei ; SPF ; Bacteria ; Parasite ; Aquaculture
    Repository Name: AquaDocs
    Type: Report , Refereed
    Format: 60pp.
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  • 7
    Publication Date: 2021-05-19
    Description: Abstract: Identification and classification of aquatic parasites are more important by the day. Aquaculture development are faced with parasites previously "not pathogenic in normal conditions, but under certain conditions bacame diseases and have caused heavy losses to the aquaculture farms. The results of these studies recognize the importance of parasites in fish production has clear economic value. Before any action for economic aquatic breeding the parasites that can infect the fish have been identified and methods of control will be drawn. The current project is a part (the fishes) of the project Parasitology Museum in the Faculty of Veterinary medicine of Tehran University. In the first phase of the project, it was planned the fish parasites from different regions of the country were collected and the Iranian Fish Parasites Data Base has also set up. Required experts and various specialties were organized for implementation an recognation of the collected parasitese. The province executive and coordination necessary and the methods of sampling were discussed after a day of training workshop was held at the Faculty of Veterinary medicine of Tehran University. Due to the lack of funds needed to coordinate the implementation, it was conducted that the parasite specimens collected from other projects. In the first phase of projects 261 parasites various have been sent. Collected samples received a temporary code and announced. In the laboratory the specimens categoried and the shape designed and finally the consultants send the final identification of the parasites. Posted parasites according to the latest international standards are maintained. Each parasite has a unique code that represents the name of the sender, verification of the final consultant, host and fishing region. Simultaneously, reported fish parasites from 1327 AD (1949 AD) were collected to create a database, they classified to be placed on the site inPersian and English. Fish parasites that have been reported are available in four types: final report of the research projects conducted at research centers and universities, student theses, abstracts published in scientific conferences and finally "published in national and international research journals. “Iranian fish parasitesd database” included: Founders and Pioneers Monument, Iranian fish parasite fauna, History, Search, Resources, Executive Committee, links, News. Researchers can search through the five “key words”: the name of the parasite, the parasite class branch, region or provincial fishing, infected of host organ. After achieving the desired list of parasites, descriptions, and specifications can be observe. The resource section lists some of the articles published and will be visible as a “pdf”.
    Description: Iranian Fisheries Science Research Institute
    Description: Published
    Keywords: Fish ; Identification ; Classification ; Aquatic parasites ; Collection
    Repository Name: AquaDocs
    Type: Report , Refereed
    Format: 68pp.
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  • 8
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    Iranian Fisheries Science Research Institute | Tehran, Iran
    In:  http://aquaticcommons.org/id/eprint/25685 | 18721 | 2018-10-14 03:04:59 | 25685 | Iranian Fisheries Science Research Institute
    Publication Date: 2021-07-16
    Description: Aquaculture is the fastest growing food industry in the world. Shrimp culture industry is also part of it, unfortunately, like other marine animal culture economic losses caused by the disease has been one of the major challenges of this industry. The major cause of mortality in shrimp hatcheries and rearing centers is related to water quality and the presence of pathogenic bacteria and parasites. These are common opportunistic microorganisms in the hatchery, rearing centers, flora and living food but poor conditions of culture are caused diseases. Since the development of aquaculture in the countries need health management, one of the important additional rings in the shrimp strategic plan is specific pathogen free shrimp production, which has been addressed in this plan. Specific pathogen free shrimp define as the shrimps which are free of the specific pathogens listed in world organization for animal health (OIE). These factors should be conclusively diagnosed and can be isolated from shrimp hatcheries and culture system. Therefore in this project screening and surveillance of shrimp in several generations according to the list of OIE were done and they were monitored for of bacterial pathogens (Necrosis Hepatopancreas Bacteria) and parasites (Microsporidian and Gregarines). At total 756 pieces of shrimp, 6 sample of dry food and 97 samples of live foods were controlled and tested. 1.35 percent of live foods were positive for NHPB and 5.6 percent of pre broodstocks have epicommensal and microsporidia which were disposed in quarantine phase. Because of biosecurity and surveillance system establishment, there were no bacterial or parasitic isolation or diagnosis during SPF shrimp production.
    Keywords: Aquaculture ; Health ; Iran ; Litopenaeus vannamei ; SPF ; Bacteria ; Parasite ; Aquaculture
    Repository Name: AquaDocs
    Type: monograph
    Format: application/pdf
    Format: application/pdf
    Format: 60
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  • 9
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    Iranian Fisheries Science Research Institute | Tehran, Iran
    In:  http://aquaticcommons.org/id/eprint/25130 | 18721 | 2018-08-26 13:37:55 | 25130 | Iranian Fisheries Science Research Institute
    Publication Date: 2021-07-16
    Description: This survey was designed in the Helleh and Delvar regions, to understand effects of aquaculture industry on coastal waters of Bushehr, in 2003. In Delvar region, five stations were selected comprise; effluent canals of Delvar 18 and 14, sea (south and north of site), influent water (Piyazi bay). In Helleh region, two stations; effluent canal and Ramleh bay were selected. Sediment and water samples were collected from effluent canal, influent canal and sea during the 9 month period (before until after culture season). The variations of selected water quality parameters were controlled monthly. Results show that, the maximum amount of ammonium in Helleh region was 0.33 mg/lit and in regions of Delvar 18 and 14 were 0.19, 0.20 mg/lit. However the maximum amounts of phosphate in Helleh region was 0.26 mg/lit and in regions of Delvar 18 and 14 were 0.24, 0.23 mg/lit. Correlation coefficients assess between phosphate concentration of effluent canal and sea station (gate of Ramleh) in Helleh region was r=0.91 (α=0.01). Also correlation coefficients
    Keywords: Aquaculture ; Iran ; Bushehr Province ; Helleh region ; Survey ; Aquaculture ; Sediment ; Samples ; Culture ; Coastal waters ; Shrimp ; Parameters ; Water quality ; Comparison
    Repository Name: AquaDocs
    Type: monograph
    Format: application/pdf
    Format: application/pdf
    Format: 122
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  • 10
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    Iranian Fisheries Science Research Institute | Tehran, Iran
    In:  http://aquaticcommons.org/id/eprint/25746 | 18721 | 2018-10-13 06:11:23 | 25746 | Iranian Fisheries Science Research Institute
    Publication Date: 2021-07-16
    Description: At present, the aquaculture industry to provide proper instructions in the field of health management, including production of Specific Pathogen Free shrimp (SPF), require sensitive and reliable methods for the detection and identification of pathogenic microorganisms. Molecular methods which used in the detection of microorganisms have a high discriminatory power in the taxonomy and in relation to libraries in the world. On the other hand, the accurate identification of microorganisms, providing the genetic data bank of shrimp pathogens and maintenance of these strains is the step to promote further research on the mechanisms of pathogenesis of pathogens, diagnosis, treatment, prevention of disease, identify indigenous production kits, diagnosis re emerging and emerging diseases and their origin. Therefore, in this project, by using ribotyping technique, native isolated pathogenic bacteria and fungi were identified and recorded in the gene bank database center. During sampling of shrimp and water of Specific Pathogen Free shrimp center, 40 bacterial strains were isolated, which 8 of them had the most frequency and identification based on 16S rDNA sequencing was performed. Bacteria identified are: Vibrio nigripulchritudo strain IS013(GenBank:KP843725), Vibrio brasiliensis strain IS014 (GenBank:KR186076), Vibrio rotiferianus strain IS015 (GenBank:KR186077), Vibrio azureus strain IS012 (GenBank:KJ018724.1), Vibrio owensii strain IS016 (GenBank:KR186078), Agarivorans gilvus strain IS017 (GenBank:KR186079), Vibrio brasiliensis IS018 (GenBank:KR186080) and Vibrio alginolyticus strain IS019 (GenBank:1817854), which were recorded in The World Bank genes. In this study fungal isolates were not detected.
    Keywords: Biology ; Iran ; Bacteria ; Fungi ; Ribotyping ; 16S rDNA ; Shrimp ; Specific pathogen free ; Vibrio rotiferianus ; Vibrio owensii ; Vibrio brasiliensis ; Vibrio azureus ; Agarivorans gilvus ; Vibrio alginolyticus ; GenBank
    Repository Name: AquaDocs
    Type: monograph
    Format: application/pdf
    Format: application/pdf
    Format: 64
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