Publication Date:
2004-11-16
Description:
Direct injection of marrow cells into injured myocardium improves cardiac function, however, repair may be limited by the number of stem cells that home and persist in the injured myocardium. Using nude rats that received a 17 min transient ligation of their left anterior descending artery, we tested whether anti-CD3 activated human T cells (ATC), G-CSF primed peripheral blood mononuclear cells (G-PBMC), or G-CSF primed purified CD34+ cells would target MI-specific injury antigens ICAM-1 or rat myosin light chain (MLC) when injected intra-jugularly 48 hrs after myocardial injury. ATC, G-PBMC, or CD34+ cells were armed (50 ng/106 cells) with anti-CD3 x anti-rat ICAM-1 or anti-CD45 x anti-MLC. Nude rats were euthanized at 24 hours or 5 weeks after treatment and hearts were frozen, sectioned, fixed, and stained accordingly. In a pilot experiment, ATC armed with OKT3 x anti-ICAM were detected at the MI sites by Cy3 staining but not in the adjacent normal myocardium 24 hrs after infusion; rats given unarmed ATC or ATC armed with anti-CD3 x anti-CD20 (irrelevant BiAb) did not stain. High numbers of cells in the MIs in rats given anti-human CD45 x anti-rat MLC-armed G-PBMC stained positive by tyramide-amplified IHC for the mouse IgG or human CD45 cells. Human cells stained for HLA-DR (〉5000 cells/injured HPF) in MIs of rats treated with BiAb-armed cells compared to unarmed-treated rats (
Print ISSN:
0006-4971
Electronic ISSN:
1528-0020
Topics:
Biology
,
Medicine
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