ISSN:
1573-5028
Keywords:
arginine biosynthesis
;
aspartate transcarbamoylase
;
carbamoyl phosphate
;
ornithine transcarbamoylase
;
Pisum sativum
Source:
Springer Online Journal Archives 1860-2000
Topics:
Biology
Notes:
Abstract We used a PCR-based library screening method to isolate a 1.4 kb pea leaf cDNA encoding ornithine transcarbamoylase (OTCase). The cDNA contains a single major ORF of 375 amino acids whose deduced sequence exhibits a high degree of homology with other OTCases. The predicted molecular mass of 41 361 Da for this protein is approximately the 40 kDa size of the polypeptide that is immunoprecipitated with OTCase antibody afterin vitro translation of pea leaf mRNA.In vivo, OTCase occurs as a trimer of identical 36.5 kDa polypeptides, suggesting that this enzyme is synthesized as a cytosolic precursor protein. Southern blot analysis indicates that multiple OTCase genes occur in pea. An abundant 1.4 kb transcript is seen in northern blots of total RNA isolated from the leaves and roots of light- and dark-grown pea seedlings.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1007/BF00040826
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