Publication Date:
2021-05-19
Description:
This study was conducted to determine the effects of dietary methionine and lysine supplementation
on digestive enzymes activity in juvenile Sobaity, Sparidentex hasta. For this purpose, 180 juvenile
fish with an initial average weight of 31.38 ± 1.4 g were distributed randomly in eighteen (300 L)
polyethylene tanks. 6 experimental diets were prepared with different levels of methionine and lysine
including control diet (without dietary methionine and lysine), Diet 1: 100% methionine; Diet 2: 75%
methionine and 25% lysine; Diet 3: 50% methionine and 50% lysine; Diet 4: 25% methionine and
75% lysine; Diet 5: 100% lysine. During the experimental period, fish were fed to satiation thrice daily
(8:00, 13:00 and 18:00 hours) for 8 weeks. At the end of the experiment, digestive enzymes (trypsin,
chymotrypsin, alkaline phosphatase, amylase, lipase and total protease were effected by different
dietary levels of methionine and lysine supplementations (P〈0/05). Also, the activity of trypsin and
chymotrypsin had showed an increasing trend with increased amount of diet lysine while enzyme
activity of alkaline phosphatase was higher in treatments contains maximum amount of methionine
supplementation. According to results, the increasing of lysine in diet reduced enzyme activity of
lipase. The results, showed no significant differences between experimental treatments in amylase and
total protease (P〈0/05). Based on the results of evaluation digestive enzymes, amino acids
supplements of lysine and methionine were changed the activity of digestive enzymes in juvenile
Sobaity, (S. hasta). Finally, Diet 2 with 75% methionine and 25% lysine was suggested for used by
Sobaity, as to the balance of dietary methionine and lysine supplementation.
Description:
Published
Keywords:
Methionine
;
Lysine
;
Sparidentex hasta
;
Intestinal
;
Digestive
;
Enzymes
;
Diet
;
Supplements
Repository Name:
AquaDocs
Type:
Journal Contribution
,
Refereed
Format:
pp.72-94
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