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  • 1
    Electronic Resource
    Electronic Resource
    Isolation of two neuropeptides in the AKH/RPCH-family from horseflies (diptera)
    Amsterdam : Elsevier
    Biochemical and Biophysical Research Communications 151 (1988), S. 656-663 
    Details
    ISSN: 0006-291X
    Keywords: AKH,; adipokinetic hormone ; CA,; corpora allata ; CC,; corpora cardiaca ; DCCI,; Dipteran corpora cardiaca factor I ; DCCII,; Dipteran corpora cardiaca factor II ; HPLC,; high performance liquid chromatography ; Hz-AKH,; Heliothis zea AKH ; PITC,; phenylisothiocyanate ; PTC,; phenylthiocarbamyl ; RPCH,; red pigment concentrating hormone ; SIR,; standard integrated response ; TEAP,; triethylammonium phosphate ; TFA,; trifluoroacetic acid ; UV,; ultraviolet
    Source: Elsevier Journal Backfiles on ScienceDirect 1907 - 2002
    Topics: Biology , Chemistry and Pharmacology , Physics
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1016/S0006-291X(88)80331-0
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  • 2
    Electronic Resource
    Electronic Resource
    Structural studies of Bacillus subtilis glutamine synthetase - Further purification, sulfhydryl groups, and the NH"2-terminal amino acid sequence
    Amsterdam : Elsevier
    Archives of Biochemistry and Biophysics 178 (1977), S. 644-651 
    Details
    ISSN: 0003-9861
    Source: Elsevier Journal Backfiles on ScienceDirect 1907 - 2002
    Topics: Biology , Chemistry and Pharmacology , Physics
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1016/0003-9861(77)90236-3
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  • 3
    Electronic Resource
    Electronic Resource
    Isolation of two neuropeptides in the AKH/RPCH-family from horseflies (diptera)
    Amsterdam : Elsevier
    Biochemical and Biophysical Research Communications 151 (1988), S. 656-663 
    Details
    ISSN: 0006-291X
    Keywords: AKH,; adipokinetic hormone ; CA,; corpora allata ; CC,; corpora cardiaca ; DCCI,; Dipteran corpora cardiaca factor I ; DCCII,; Dipteran corpora cardiaca factor II ; HPLC,; high performance liquid chromatography ; Hz-AKH,; Heliothis zea AKH ; PITC,; phenylisothiocyanate ; PTC,; phenylthiocarbamyl ; RPCH,; red pigment concentrating hormone ; SIR,; standard integrated response ; TEAP,; triethylammonium phosphate ; TFA,; trifluoroacetic acid ; UV,; ultraviolet
    Source: Elsevier Journal Backfiles on ScienceDirect 1907 - 2002
    Topics: Biology , Chemistry and Pharmacology , Physics
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1016/S0006-291X(88)80331-0
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  • 4
    Electronic Resource
    Electronic Resource
    Interaction of a radiolabeled cytokinin photoaffinity probe with a receptor protein
    Amsterdam : Elsevier
    Biochemical and Biophysical Research Communications 96 (1980), S. 1325-1334 
    Details
    ISSN: 0006-291X
    Source: Elsevier Journal Backfiles on ScienceDirect 1907 - 2002
    Topics: Biology , Chemistry and Pharmacology , Physics
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1016/0006-291X(80)90096-0
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  • 5
    Electronic Resource
    Electronic Resource
    The RecE recombination pathway mediates recombination between partially homologous DNA sequences: Structural analysis of recombination products
    Amsterdam : Elsevier
    Journal of Structural Biology 104 (1990), S. 97-106 
    Details
    ISSN: 1047-8477
    Source: Elsevier Journal Backfiles on ScienceDirect 1907 - 2002
    Topics: Biology
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1016/1047-8477(90)90063-I
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  • 6
    Electronic Resource
    Electronic Resource
    Genetic mapping of soybean cyst nematode race-3 resistance loci in the soybean PI 437.654 (1995)
    Springer
    Theoretical and applied genetics 91 (1995), S. 574-581 
    Details
    ISSN: 1432-2242
    Keywords: Glycine max ; Heterodera glycines ; RFLP ; Genetic mapping
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Resistance to the soybean cyst nematode (SCN) (Heterodera glycines Ichinohe) is difficult to evaluate in soybean [Glycine max (L.) Merr.] breeding. PI 437.654 has resistance to more SCN race isolates than any other known soybean. We screened 298 F6∶7 recombinant-inbred lines from a cross between PI 437.654 and ‘BSR101’ for SCN race-3 resistance, genetically mapped 355 RFLP markers and the I locus, and tested these markers for association with resistance loci. The Rhg 4 resistance locus was within 1 cM of the I locus on linkage group A. Two additional QTLs associated with SCN resistance were located within 3cM of markers on groups G and M. These two loci were not independent because 91 of 96 lines that had a resistant-parent marker type on group G also had a resistant-parent marker type on group M. Rhg 4 and the QTL on G showed a significant interaction by together providing complete resistance to SCN race-3. Individually, the QTL on G had greater effect on resistance than did Rhg 4, but neither locus alone provided a degree of resistance much different from the susceptible parent. The nearest markers to the mapped QTLs on groups A and G had allele frequencies from the resistant parent indicating 52 resistant lines in this population, a number not significantly different from the 55 resistant lines found. Therefore, no QTLs from PI 437.654 other than those mapped here are expected to be required for resistance to SCN race-3. All 50 lines that had the PI 437.654 marker type at the nearest marker to each of the QTLs on groups A and G were resistant to SCN race-3. We believe markers near to these QTLs can be used effectively to select for SCN race-3 resistance, thereby improving the ability to breed SCN-resistant soybean varieties.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00223282
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  • 7
    Electronic Resource
    Electronic Resource
    Restriction fragment length polymorphisms as genetic markers in soybean, Glycine max (L.) merrill (1988)
    Springer
    Theoretical and applied genetics 75 (1988), S. 889-901 
    Details
    ISSN: 1432-2242
    Keywords: Soybean ; Restriction fragment length polymorphism ; Genetics ; Allele ; Variation
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary Restriction Fragment Length Polymorphisms (RFLP) have been identified between widely distant cultivars (‘Minsoy’ and ‘Noir 1 ’) of soybean Glycine max (L.) Merrill. Using as probes randomly chosen clones of DNA, one in five probes revealed a polymorphism. More than half of these polymorphisms appear to result from rearrangements of the genomic DNA. Twenty seven markers were analyzed for linkage in F2 plants. Eleven of these markers were contained in four linkage groups. Five cultivars were compared in a search for new alleles. When RFLP markers corresponding to low copy DNA were used to analyze three other cultivars — ‘Sooty’, ‘Forrest’ and ‘Mandarin (Ottawa)’ — few new alleles were found. Using these probes, five different markers could be used to differentiate the five cultivars. Complex probes, which correspond to repeated DNA, revealed different polymorphisms in different cultivars and a single such probe could be used to distinguish the five cultivars from each other.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00258050
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  • 8
    Electronic Resource
    Electronic Resource
    Allele-specific hybridization markers for soybean (1999)
    Springer
    Theoretical and applied genetics 98 (1999), S. 690-696 
    Details
    ISSN: 1432-2242
    Keywords: Key words Glycine max ; Soybean ; Allele-specific hybridization ; PCR ; Marker-assisted selection
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract  Soybean (Glycine max) is one of the world’s most important crop plants due to extensive genetic improvements using traditional breeding approaches. Recently, marker-assisted selection has enhanced the ability of traditional breeding programs to improve soybeans. Most methods of assessing molecular markers involve electrophoretic techniques that constrain the ability to perform high-throughput analyses on breeding populations and germplasm. In order to develop a high-capacity system, we have developed allele-specific hybridization (ASH) markers for soybean. As one example, restriction fragment length polymorphism (RFLP) locus A519-1 (linkage group B) was converted into an ASH marker by (1) sequencing the pA519 cloned insert, (2) designing locus-specific PCR amplification primers, (3) comparative sequencing of A519-1 amplicons from important soybean ancestors, and (4) designing allele-specific oligonucleotide probes around single nucleotide polymorphisms (SNPs) among soybean genotypes. Two SNPs were identified within approximately 400 bp of the sequence. Allele-specific probes generated a 100-fold greater signal to target amplicons than to targets that differed by only a single nucleotide. The A519-1 ASH marker is shown to cosegregate with the A519-1 RFLP locus. In order to determine ASH usefulness, we genotyped 570 soybean lines from the Pioneer Hi-Bred soybean improvement using both A519-1 SNPs. Combined haplotype diversity (D) was 0.43 in this adapted germplasm set. These results demonstrate that ASH markers can allow for high-throughput screening of germplasm and breeding populations, greatly enhancing breeders’ capabilities to do marker-assisted selection.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/s001220051122
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  • 9
    Electronic Resource
    Electronic Resource
    Rpg1, a soybean gene effective against races of bacterial blight, maps to a cluster of previously identified disease resistance genes (1998)
    Springer
    Theoretical and applied genetics 96 (1998), S. 1013-1021 
    Details
    ISSN: 1432-2242
    Keywords: Key words Nucleotide binding site ; Leucine-rich repeats ; RFLP mapping ; Recombinant-inbred lines
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract  Alleles, or tightly linked genes, at the soybean (Glycine max L. Merr.) Rpg1 locus confer resistance to races of Pseudomonas syringae pv. glycinea that express the avirulence genes avrB or avrRpm1. In this study we demonstrate that Rpg1 maps to a cluster of previously identified resistance genes, including those effective against fungal, viral and nematode pathogens. Rpg1 is in molecular linkage group (MLG) F, flanked by the markers K644 and B212. The RFLP markers R45, php2265 and php2385 cosegregated with Rpg1, as did the marker nbs61, which encodes a protein related to previously isolated resistance genes.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/s001220050833
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  • 10
    Electronic Resource
    Electronic Resource
    A Genetic linkage map of Pinyon pine (Pinus edulis) based on amplified fragment length polymorphisms (1998)
    Springer
    Theoretical and applied genetics 97 (1998), S. 871-880 
    Details
    ISSN: 1432-2242
    Keywords: Key words AFLP ; Linkage map ; Pinus edulis
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract  Amplified fragment length polymorphisms (AFLP) were used to rapidly generate a dense linkage map for pinyon pine (Pinus edulis). The map population consisted of 40 megagametophytes derived from one tree at Sunset Crater, Arizona. A total of 78 primer combinations, each with three to five selective nucleotides, amplified 542 polymorphic markers. Of these, 33 markers showed significant deviation from the expected Mendelian genotypic segregation ratio of 1 : 1, and 164 showed complete linkage with another marker. This resulted in 338 unique markers mapping to 25 linkage groups, each of which ranged from 2 to 22 markers, averaging 80 centiMorgans (cM) in size and covering 2,012 cM (2,200 cM with the inclusion of 25 cM for each of 7 unlinked markers). Pairwise linkage values gave a genome size estimate of 2,390 cM, suggesting comprehensive coverage of the genome. A search for subsets of primer combinations giving the best map coverage found 10 primer combinations which together marked 72% of the linkage map to within 10 cM; an additional 10 primer combinations increased this percentage to 85%. Our map represents an initial step towards the identification of quantitative trait loci associated with pest resistance and water stress in pinyons and will further allow us to examine introgression rates between P. edulis and P. californiarum.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/s001220050967
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