ISSN:
1432-0878
Keywords:
Memory
;
Optic nerve
;
Regeneration, CNS
;
Ependymin
;
Cerebrospinal fluid
;
Meninges
;
Neuronal plasticity
;
In situ hybridization
;
Carassius auratus (Teleostei)
Source:
Springer Online Journal Archives 1860-2000
Topics:
Biology
,
Medicine
Notes:
Summary Ependymins are dimeric glycoproteins found in the extracellular fluid of goldfish brain. They were originally observed because of their enhanced turnover rates after learning. In this paper we present the first investigation concerning the expression of these secretory proteins in goldfish brain via in situ hybridization with synthetic oligonucleotides and cRNA probes. It is shown that ependymin-mRNAs are predominantly expressed in the meninx surrounding the brain and in an invaginated part of the meninx called the cavum cranii. These results have been confirmed by immunhistochemical analysis. This indicates that, in fish, the meninx synthesizes a major protein constituent of the cerebrospinal fluid; furthermore, this suggests that the functional sites of ependymins are removed from the place of their synthesis. Distribution between different compartments may be achieved via the open communication system of the perivascular spaces.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1007/BF00329438
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