ISSN:
0025-116X
Keywords:
Chemistry
;
Polymer and Materials Science
Source:
Wiley InterScience Backfile Collection 1832-2000
Topics:
Chemistry and Pharmacology
,
Physics
Notes:
The small-angle X-ray scattering of L-leucine dehydrogenase (EC 1.4.1.9) from Bacillus sphaericus in 0,2 M sodium phosphate buffer (pH 7,28) was investigated. The following molecular parameters were determined: radius of gyration, as 50,8 Å from the Guinier plot and 48,5 Å from the distance distribution function; molecular weight, as 267 000; volume, as 623 000 Å3; and degree of hydration, as 0,95 g H2O/g of enzyme. By comparing the observed scattering curve with those calculated with a number of models, a hollow cylinder with 47,0 Å of outer radius, 10,3 Å of inner radius and 119,0 Å of length was proposed as a most plausible model for the enzyme.
Additional Material:
5 Ill.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1002/macp.1982.021830323
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