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  • 1
    Electronic Resource
    Electronic Resource
    Somatic hybrids between Solanum brevidens and Solanum tuberosum: Expression of a late blight resistance gene and potato leaf roll resistance (1986)
    Springer
    Plant cell reports 5 (1986), S. 212-214 
    Details
    ISSN: 1432-203X
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Hexaploid somatic hybrids resulting from mesophyll protoplast fusions between Solanum brevidens Phil., PI 218228, and Solanum tuberosum L., PI 203900 were tested for late blight resistance using two races of Phytophthora infestans Monte., de Bary. The S. tuberosum parent was a “late blight differential” possessing the R4 gene which confers resistance to race 0. The S. brevidens parent is resistant to potato leaf roll virus. Inoculations with both compatible (race 1.3.4.5) and incompatible (race 0) races of P. infestans clearly demonstrated the expression of the late blight resistance gene in all of the hybrid progeny tested. Most of the hybrids tested were also resistant to potato leaf roll virus (PLRV), indicating that the S. brevidens genes for PLRV resistance were present and expressed.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00269122
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  • 2
    Electronic Resource
    Electronic Resource
    Resistance to late blight in Solanum bulbocastanum is mapped to chromosome 8 (2000)
    Springer
    Theoretical and applied genetics 101 (2000), S. 697-704 
    Details
    ISSN: 1432-2242
    Keywords: Key words Solanum bulbocastanum ; Late blight ; Phytophthora infestans ; Somatic hybrid
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract  Somatic hybrids between potato and Solanum bulbocastanum, a wild diploid (2n=2x=24) Mexican species, are highly resistant to late blight, caused by Phytophthora infestans. Both randomly amplified polymorphic DNA (RAPD) and restriction fragment length polymorphism (RFLP) markers that are closely linked to the resistance have been noted by analysis of three different backcross-2 populations derived from two different somatic hybrids. With reference to previously published potato and tomato maps, resistance appears to be on the long arm of chromosome 8 and is flanked by RFLP markers CP53 and CT64. In a population of BC2 plants derived from a cross between the BC1 line J10lK6 [(S. tuberosum PI 203900+S. bulbocastanum PI 243510) ×Katahdin)]×Atlantic, late blight resistance cosegregated with RFLP marker CT88 and RAPD marker OPG02–625.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/s001220051533
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  • 3
    Electronic Resource
    Electronic Resource
    Development and applications of a set of chromosome-specific cytogenetic DNA markers in potato (2000)
    Springer
    Theoretical and applied genetics 101 (2000), S. 1001-1007 
    Details
    ISSN: 1432-2242
    Keywords: Key words Chromosome identification ; Physical mapping ; FISH ; BAC ; Molecular cytogenetics ; Potato
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract  Reliable and easy to use techniques for chromosome identification are critical for many aspects of cytogenetic research. Unfortunately, such techniques are not available in many plant species, especially those with a large number of small chromosomes. Here we demonstrate that fluorescence in situ hybridization (FISH) signals derived from bacterial artificial chromosomes (BACs) can be used as chromosome-specific cytogenetic DNA markers for chromosome identification in potato. We screened a potato BAC library using genetically mapped restriction fragment length polymorphism markers as probes. The identified BAC clones were then labeled as probes for FISH analysis. A set of 12 chromosome-specific BAC clones were isolated and the FISH signals derived from these BAC clones serve as convenient and reliable cytological markers for potato chromosome identification. We mapped the 5S rRNA genes, the 45S rRNA genes, and a potato late blight resistance gene to three specific potato chromosomes using the chromosome-specific BAC clones.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/s001220051573
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  • 4
    Electronic Resource
    Electronic Resource
    Fertility of somatic hybrids from protoplast fusions of Solanum brevidens and S. tuberosum (1987)
    Springer
    Theoretical and applied genetics 73 (1987), S. 395-402 
    Details
    ISSN: 1432-2242
    Keywords: Protoplast ; Somatic fusion ; Solanum brevidens ; Solanum tuberosum ; Endosperm balance number
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary Two sets of somatic hybrids between Solanum brevidens (2x) and S. tuberosum (2x and 4x) were evaluated for male fertility, meiotic regularity and female fertility. The somatic hybrids were tetraploids from 2x + 2x fusions and hexaploids from 2x + 4x fusions. Pollen stainability ranged from 0 to 83% in tetraploids and from 0 to 23% in hexaploids. The tetraploids had more regular meiosis, lower levels of micropollen and fewer unassociated chromosomes than hexaploids. However, except for a low level of selfing, the pollen of both sets of hybrids was ineffective in pollinations. The tetraploids, as females, crossed poorly with 2x and 4x tester species and selfed only at low levels. The hexaploid fusion hybrids also crossed poorly with the 2x tester species and selfed only to a limited degree; however, they crossed well with 4x testers. Seed set in crosses with S. tuberosum Group Andigena, and S. tuberosum Group Tuberosum cultivars ‘Kathadin’ and ‘Norland’ averaged 16.7, 15.6 and 28.6 seeds per fruit, respectively. Progeny from these crosses had 5x or nearly 5x ploidy levels. The results indicate that reasonable levels of female fertility can be obtained in somatic fusion hybrids of S. brevidens and S. tuberosum.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00262507
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  • 5
    Electronic Resource
    Electronic Resource
    Recombination of Solanum brevidens chromosomes in the second backcross generation from a somatic hybrid with S. tuberosum (1994)
    Springer
    Theoretical and applied genetics 88 (1994), S. 917-924 
    Details
    ISSN: 1432-2242
    Keywords: Potato ; Introgression ; Wide hybrids ; RFLP RAPD
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Solanum brevidens synteny groups were examined with 47 widely-distributed RFLP markers in 17 BC2 progeny from six fertile BC1 plants. The BC1 plants were derived from a single S. brevidens + S. tuberosum somatic hybrid backcrossed with S. tuberosum (potato). Probes which were linked in potato and tomato were also found to be syntenic along each of the 12 S. brevidens chromosomes. More than half of the S. brevidens synteny groups had lost one or more S. brevidens-specific RFLPs in the BC2, suggesting that recombination had occurred. For 8 of the 12 S. brevidens RFLP synteny groups, the frequency of recombinant chromosomes exceeded that of intact parental chromosomes. Using the RFLP data, 161 RAPD markers were tentatively located throughout the S. brevidens genome. Further analyses with 39 of these 161 RAPD markers generally showed that RAPD and RFLP results were comparable, but some inconsistencies were noted with 14 of the 39 RAPD markers. The extent of marker loss and the high frequency of synteny groups which were marked by a single S. brevidens-specific RFLP marker suggest that the S. brevidens chromosomes have some pairing affinity with potato chromosomes. This interaction should facilitate the transfer of novel disease-resistance traits into potato breeding lines. One plant was recovered with the chromosome number of S. tuberosum (2n=48) that carried a single S. brevidens RFLP marker, suggesting transfer of this S. brevidens marker into the genome of S. tuberosum.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00220797
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  • 6
    Electronic Resource
    Electronic Resource
    Intra-specific fusions in Solanum tuberosum (1985)
    Springer
    Theoretical and applied genetics 71 (1985), S. 172-175 
    Details
    ISSN: 1432-2242
    Keywords: Protoplast ; Somatic fusion ; Overcoming incompatibility ; S. tuberosum
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary Plants were regenerated from callus arising from protoplast fusion of two S. tuberosum diploids. Tetraploid progeny from the fusion of the two diploid partners had increased vigor. Isozyme analysis confirmed the presence of proteins from both partners in the fusion progeny. Pigmentation of tubers and anthers was heightened substantially in the fusion products. This fusion, the first intra-specific fusion within S. tuberosum, indicates that somatic fusion may be useful for transferring traits within this group.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00252052
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  • 7
    Electronic Resource
    Electronic Resource
    Somatic hybrids produced by protoplast fusion between S. tuberosum and S. brevidens: phenotypic variation under field conditions (1986)
    Springer
    Theoretical and applied genetics 71 (1986), S. 682-690 
    Details
    ISSN: 1432-2242
    Keywords: S. tuberosum ; Protoplast ; Somatic fusion ; Variation in hybrids
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary Phenotypic and flowering characteristics of hybrid plants generated by protoplast fusion between a tetraploid S. tuberosum line and diploid S. brevidens were assessed under field conditions. Hybrids were compared to both clonal parental material and protoplast-derived plants of each parent. Almost all of the hybrids were hexaploid. A wide range of variation in morphological characters was observed for hybrids and protoclones. Flowering was markedly reduced in protoclones. The majority of hybrids flowered, had viable pollen and set tubers. Tuber and pollen characteristics of hybrids produced from individual fusion calli also varied. The potential usefulness of fusion hybrids in potato improvement is discussed.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00263264
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  • 8
    Electronic Resource
    Electronic Resource
    Somatic hybrids between Solanum etuberosum and diploid, tuber bearing Solanum clones (1994)
    Springer
    Theoretical and applied genetics 89 (1994), S. 775-782 
    Details
    ISSN: 1432-2242
    Keywords: Somatic hybrid ; Solanum etuberosum ; S. tuberosum ; S. berthaultii ; RFLP
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Electrofusion was used to obtain somatic hybrids between Solanum etuberosum (2n=2x=24) and two diploid potato lines. These hybridizations were conducted to determine if haploidxwild species hybrids are better fusion partners than conventional S. tuberosumGp. Tuberosum haploids. Restriction fragment length polymerase (RFLP) analyses of the putative somatic hybrids confirmed that each parental genome was present. The somatic hybrids between S. etuberosum and a haploid S. tuberosum clone, US-W730, were stunted and had curled, purple leaves. In contrast, somatic hybrids between S. etuberosum and a haploidxwild species hybrid (US-W 730 haploidx S. berthaultii), were vigorous and generally tuberized under field conditions. These hybrids were designated as E+BT somatic hybrids. Analyses of 23 E+BT somatic hybrids revealed a statistically significant bias towards the retention of S. etuberosum chloroplasts. Stylar incompatibilities were observed when the E+BT somatic hybrids were used as pollen donors in crosses with S. tuberosum cultivars. Reciprocal crosses did not show this incompatibility. The progeny were vigorous and had improved tuber traits when compared to the maternal E+BT parent. RFLP analyses of three sexual progeny lines confirmed the presence of all 12 S. etuberosum chromosomes. In two of these lines, RFLPs that marked each of the 24 chromosome arms of S. etuberosum were present. However, RFLP markers specific for regions on chromosomes 2, 7, and 11 were missing from the third clone. Because other markers for these chromosomes were present in the progeny line, these results indicated the likelihood of pairing and recombination between S. etuberosum and S. tuberosum chromosomes.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00223718
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  • 9
    Electronic Resource
    Electronic Resource
    Somatic hybrids between Solanum bulbocastanum and potato: a new source of resistance to late blight (1998)
    Springer
    Theoretical and applied genetics 96 (1998), S. 738-742 
    Details
    ISSN: 1432-2242
    Keywords: Key words Somatic hybrid ; Solanum bulbocastanum ; Solanum tuberosum ; Late blight ; Phytophthora infestans
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract  Solanum bulbocastanum, a wild, diploid (2n=2x=24) Mexican species, is highly resistant to Phytophthora infestans, the fungus that causes late blight of potato. However this 1 EBN species is virtually impossible to cross directly with potato. PEG-mediated fusion of leaf cells of S. bulbocastanum PI 245310 and the tetraploid potato line S. tuberosum PI 203900 (2n=4x=48) yielded hexaploid (2n= 6x=72) somatic hybrids that retained the high resistance of the S. bulbocastanum parent. RFLP and RAPD analyses confirmed the hybridity of the materials. Four of the somatic hybrids were crossed with potato cultivars Katahdin or Atlantic. The BC1 progeny segregated for resistance to the US8 genotype (A-2 mating type) of P. Infestans. Resistant BC1 lines crossed with susceptible cultivars again yielded populations that segregated for resistance to the fungus. In a 1996 field-plot in Wisconsin, to which no fungicide was applied, two of the BC1 lines, from two different somatic hybrids, yielded 1.36 and 1.32 kg/plant under a severe late-blight epidemic. In contrast, under these same conditions the cultivar Russet Burbank yielded only 0.86 kg/plant. These results indicate that effective resistance to the late-blight fungus in a sexually incompatible Solanum species can be transferred into potato breeding lines by somatic hybridization and that this resistance can then be further transmitted into potato breeding lines by sexual crossing.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/s001220050796
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  • 10
    Electronic Resource
    Electronic Resource
    Fertile Solanum tuberosum+S. commersonii somatic hybrids as sources of resistance to bacterial wilt caused by Ralstonia solanacearum (1999)
    Springer
    Theoretical and applied genetics 98 (1999), S. 1272-1278 
    Details
    ISSN: 1432-2242
    Keywords: Key words EBN (endosperm balance number) ; Somatic hybrids ; Potato ; Brown rot ; Pseudomonas solanacearum
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract  The wild potato relative Solanum commersonii is reported to carry resistance to bacterial wilt disease caused by Ralstonia solanacearum. To overcome sexual incompatibilites due to differences in ploidy and endosperm balance numbers, somatic hybrids were made that combine the S. tuberosum and S. commersonii genomes. The resulting somatic hybrid plants are vigorous, but their disease resistance level and their fertility was unknown. We therefore tested the S. commersonii and S. tuberosum source material cv Superior, potato cv Atlantic and six somatic hybrid lines for resistance to a virulent strain of R. solanacearum (race 3, biovar 2) at 28°C. As expected, S. commersonii was significantly more wilt-resistant than the cultivated potatoes. In five of the six somatic hybrid lines, disease resistance levels were similar to that of the resistant S. commersonii parent. The resistance level of the sixth somatic hybrid was intermediate, significantly different from both S. commersonii and S. tuberosum. In controlled crosses, the somatic hybrids in this study proved both to be male- and female-fertile and were self-compatible. More importantly, the somatic hybrids can be crossed with S. tuberosum to produce viable seeds.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/s001220051193
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