ISSN:
1662-8985
Quelle:
Scientific.Net: Materials Science & Technology / Trans Tech Publications Archiv 1984-2008
Thema:
Maschinenbau
Notizen:
In the Lusatia area a pilot plant for the treatment of acidic mine waters by microbial ironoxidation and a concomitant iron hydroxysulfate precipitation is operated.Molecular based studies of acidic waters from this iron hydroxysulfate producing pilot plantrevealed the presence of 16S rRNA gene sequences from undescribed iron-oxidizing bacteria. Mostof these were related to autotrophic Betaproteobacteria (see Heinzel et al. IBS 2007, poster number98).For cultivating different iron-oxidizing bacteria water samples from this pilot plant were directlyplated on various solid media. Double-layer plates were used, with a heterotrophic Acidiphiliumstrainin the underlayer, because of the high sensitivity of autotrophic bacteria towards organicsubstances. The media contained different iron-substrates with and without organic carbon sources.Colonies appeared at the latest after three weeks and they were encrusted with ferric iron. Thephylogeny of the isolated strains was determined and the physiological requirements, liketemperature, pH optima, preferred carbon source and iron concentrations, were analyzed. Manyisolates which were related to Acidithiobacillus ferrooxidans strains could be cultivated, as well asan isolate related to the genus Thiomonas and one isolate related to a Ferribacter polymyxa species.The Thiomonas-like isolate showed best growth in media containing tryptone soya broth, sodiumthiosulfate and ferrous sulfate at pH 2.5 and 30°C. The other Betaproteobacterium grew on ferroussulfate medium at pH 2.5 between 16 and 37°C. In liquid culture experiments the cells of bothisolates were attached to the iron minerals built in the medium. These physiological characteristicsof the isolates helped to vary parameters in the pilot plant to optimize the process of iron oxidationand improved waste water remediation
Materialart:
Digitale Medien
URL:
http://www.tib-hannover.de/fulltexts/2011/0528/01/39/transtech_doi~10.4028%252Fwww.scientific.net%252FAMR.20-21.582.pdf
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