ISSN:
1573-8469
Keywords:
antibody
;
ELISA
;
Plant virus
;
RBDV
;
Rosaceae
Source:
Springer Online Journal Archives 1860-2000
Topics:
Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
Notes:
Abstract Raspberry bushy dwarf virus (RBDV) was detected in three new host plants inRubus species,i.e., arctic bramble (R. arcticus ssp.arcticus), Alaskan arctic bramble (R. arcticus ssp.stellatus) and their hybrid (R. arcticus L. nothosubsp.stellarcticus G. Larsson). The virus was identified as RBDV by the symptoms elicited in the test plantsChenopodium quinoa andC. amaranticolor, by sedimentation profile in sucrose density gradient, by RNA banding pattern in agarose gel electrophoresis, by protein analysis of the purified viruses in SDS-polyacrylamide gel electrophoresis, and by Western blotting. There was a high incidence of RBDV-infected plants in the experimental plots. The presence of the virus in arctic bramble did not always induce foliar symptoms. However, yellowing of the leaves around central and lateral veins was quite frequently observed, especially in spring and autumn.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1007/BF01877253
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