Publication Date:
2014-10-25
Description:
Pyridoxal kinases (PdxK) catalyze the phosphorylation of vitamin B6precursors. Thus, these enzymes are an essential part of many metabolic processes in all organisms. The protozoan parasitePlasmodium falciparum(the main causative agent of Malaria tropica) possesses a uniquede novoB6-biosynthesis pathway in addition to a interconversion pathway based on the activity of plasmodial PdxK (PfPdxK). The role of PdxK in B6salvage has prompted previous authors to suggest PdxK as a promising target for structure-based antimalarial drug design. Here, the expression, purification, crystallization and preliminary X-ray diffraction analysis ofPfPdxK are reported.PfPdxK crystals have been grown in space groupP21, with unit-cell parametersa= 52.7,b= 62.0,c= 93.7 Å, β = 95°. A data set has been collected to 2 Å resolution and an initial molecular-replacement solution is described.
Electronic ISSN:
2053-230X
Topics:
Biology
,
Chemistry and Pharmacology
,
Geosciences
,
Physics
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