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  • 1
    Electronic Resource
    Electronic Resource
    Isolation and study of two strains ofLeptospirillum-like bacteria from a natural mixed population cultured on a cobaltiferous pyrite substrate (1994)
    Springer
    Antonie van Leeuwenhoek 66 (1994), S. 295-302 
    Details
    ISSN: 1572-9699
    Keywords: Leptospirillum ; pyrite ; cobalt ; iron ; leaching
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Two strains ofLeptospirillum-like bacteria, L6 and L8, have been isolated from a mixed inoculum, also containingThiobacillus ferrooxidans andT. thiooxidans, cultured for one year with a colbaltiferous pyrite as energy substrate in a 100 I continuous bioleaching laboratory unit. Several physiological properties of the strains are described. The vibrio-shaped microorganisms grew at pH values lower than 1.3. Their growth rate was maximum between 2.5 and 8.0 g l1 ferrous iron. The optimal growth temperature was 37.5° C. Ferric iron had a stimulative effect on bacterial development up to 8 g l−1, and growth was as rapid at 14 g l−1 ferric iron as at 8 g l−1. The negative influence of cobalt on the final cell concentration was observed at 0.5 g l−1, but the growth rate was not affected up to 2 g l−1. The G + C content of strains L8 is 55.6 mol%.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00882763
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  • 2
    Electronic Resource
    Electronic Resource
    The use of Aspergillus niger for the bioconversion of olive mill waste-waters (1991)
    Springer
    Applied microbiology and biotechnology 34 (1991), S. 828-831 
    Details
    ISSN: 1432-0614
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Process Engineering, Biotechnology, Nutrition Technology
    Notes: Summary Olive mill waste-water was used for protein production in small-scale experiments, using non-sterilized medium without pH control. A 14 g/1 concentration of proteins, 61% chemical oxygen demand removal and a 58% reduction in total phenolic compounds were obtained using an Aspergillus niger strain. The removal of phenolic compounds resulted in a change in the colour of the waste-water from black to brown.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00169359
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  • 3
    Electronic Resource
    Electronic Resource
    Thermophilic methanogenesis from gelatin by a mixed defined bacterial culture (1986)
    Springer
    Applied microbiology and biotechnology 24 (1986), S. 79-83 
    Details
    ISSN: 1432-0614
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Process Engineering, Biotechnology, Nutrition Technology
    Notes: Summary The fermentation of gelatin by different associations of bacteria, including Thermobacteroides proteolyticus, Methanobacterium sp. and Methanosarcina MP was studied. Experimental vessels were incubated at 55°C. T. proteolyticus growing axenically produced acetate, isovalerate, H2 and CO2. Traces of propionate and isobutyrate were detected. Cocultures of T. proteolyticus and Methanobacterium sp. showed an increase in propionate and isobutyrate production. The Thermobacteroides-Methanosarcina association had no effect on metabolism of T. proteolyticus, and acetate was not used. In triculture, growth of Methanosarcina MP occurred on acetate in coculture with T. proteolyticus and Methanobacterium sp. Utilization of H2 by Methanobacterium sp. in the triculture lowered the H2 concentration sufficiently to permit acetate utilization by Methanosarcina. Maximum methane production was obtained with the triculture system.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00266290
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  • 4
    Electronic Resource
    Electronic Resource
    Isolation and characterization ofSporomusa acidovorans sp. nov., a methylotrophic homoacetogenic bacterium (1985)
    Springer
    Archives of microbiology 142 (1985), S. 307-310 
    Details
    ISSN: 1432-072X
    Keywords: Sporomusa acidovorans ; Homoacetogenesis ; Methanol ; Hydrogen ; Fructose
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Sporomusa acidovorans sp.nov. was isolated from a pilot fermenter inoculated with effluent sample from the alcohol distillation industry. The isolate was a Gram-negative, motile, curved, spore-forming rod. The DNA base composition was 42% G+C. The temperature range for growth was 20 to 40°C, with an optimum at 35°C; growth occurred within a pH range of 5.4 to 7.5, with an optimum at pH 6.5. Growth substrates included methanol, H2−CO2, formate, fructose, ribose, fumarate, succinate and glycerol. Yeast extract was required for growth. The organism performed the homoacetogenic reaction.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00693409
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  • 5
    Electronic Resource
    Electronic Resource
    Reduction of oxidized inorganic nitrogen compounds by a new strain of Thiobacillus denitrificans (1975)
    Springer
    Archives of microbiology 103 (1975), S. 31-36 
    Details
    ISSN: 1432-072X
    Keywords: Thiobacillus denitrificans ; Reduction of Nitrate ; Nitrite ; Nitric Oxide ; Nitrous Oxide
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Denitrification by Thiobacillus denitrificans “RT” strain was investigated using manometry and gas chromatography. 1. From nitrate, resting cells produced only nitrogen anaerobically with thiosulfate as the electron donor. The data suggest that nitrate was assimilated and dissimilated by the same nitrate reductase, assayed with benzyl-viologen as the electron donor. 2. From nitrite, whole cells produced nitric oxide, nitrous oxide and nitrogen, using thiosulfate as the electron donor; nitrogen was the final product of the reduction. Crude extract reduced nitrite to nitrogen with p-phenylenediamine and dimethyl-p-phenylene diamine as the electron donors, and produced nitric oxide, nitrous oxide and nitrogen with tetramethyl-p-phenylene-diamine as the electron donor. Nitrite was reduced to nitric oxide and nitrous oxide by crude extract using ascorbate-phenazine methosulfate as the electron donor. 3. From nitric oxide, whole cells produced nitrous oxide and nitrogen using thiosulfate as the electron donor, nitrogen was the final reduction product. Nitric oxide was reduced to nitrous oxide by crude extract with the ascorbatephenazine methosulfate system. 4. Whole cells reduced nitrous oxide to nitrogen with thiosulfate as the electron donor. It was not possible to detect any nitrous oxide reductase activity in crude extract. 5. A scheme was formulated as a possible mechanism of denitrification by Thiobacillus denitrificans “RT” strain.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00436326
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  • 6
    Electronic Resource
    Electronic Resource
    Sulfate reduction and anaerobic glycerol degradation by a mixed microbial culture (1991)
    Springer
    Current microbiology 22 (1991), S. 47-52 
    Details
    ISSN: 1432-0991
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Abstract Anaerobic glycerol degradation by a mixed microbial culture from a fermenter fed with industrial alcohol distillation waste water, was investigated in the absence or presence of sulfate, at 37°C and at a constant pH of 7.2. In the absence of sulfate, glycerol utilization was found to be characterized by the transient formation of 1,3-propanediol prior to propionate and acetate accumulation. In the presence of sulfate, 1,3-propanediol production was minor, and the carbon balance reflected a considerable accumulation of intermediate(s). A study of the role of sulfate reduction and methanogenesis on anaerobic 1,3-propanediol degradation showed that consumption of this substrate by the mixed microbial culture required a terminal electron acceptor. The number of fermentative and sulfate-reducing bacteria with glycerol or 1,3-propanediol as carbon and energy source revealed that sulfate-reducing bacteria outcompete fermentative bacteria for these substrates. The possible ecological role of sulfate-reducing bacteria in the metabolism of these reduced substrates is discussed.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF02106212
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  • 7
    Electronic Resource
    Electronic Resource
    Anaerobic Degradation of Glycerol by Desulfovibrio fructosovorans and D. carbinolicus and Evidence for Glycerol-Dependent Utilization of 1,2-Propanediol (1998)
    Springer
    Current microbiology 36 (1998), S. 283-290 
    Details
    ISSN: 1432-0991
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Abstract. The degradation of glycerol by Desulfovibrio carbinolicus and Desulfovibrio fructosovorans was tested in pure culture with sulfate and in coculture with Methanospirillum hungatei. Desulfovibrio carbinolicus degraded glycerol into 3-hydroxypropionate with the formation of sulfide in pure culture and methane in the coculture. The maximum growth rates were 0.063 h−1 in pure culture and 0.014 h−1 in coculture (corresponding growth yields: 8.9 and 6.0 g dry weight/mol glycerol). With D. fructosovorans, the pathway of glycerol degradation depended upon the terminal electron acceptor. Acetate and sulfide were produced in the presence of sulfate, while 3-hydroxypropionate and methane were formed by the syntrophic association with M. hungatei. The maximum growth rates were 0.057 h−1 in pure culture and 0.020 h−1 in coculture (corresponding growth yields: 8.9 and 6.0 g dry weight/mol glycerol). In a medium containing both glycerol and 1,2-propanediol but no sulfate, D. carbinolicus and D. fructosovorans degraded both substrates. A drop in the concentration of 1,3-propanediol was observed, and propionate and n-propanol production was recorded. Putative biochemical pathways of 1,2-propanediol degradation by D. carbinolicus and D. fructosovorans indicated that the enzymes involved in this metabolism are present only when the strains are grown on a mixture of 1,2-propanediol and glycerol without sulfate.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/s002849900311
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  • 8
    Electronic Resource
    Electronic Resource
    Thermophilic methanogenesis from pectin by a mixed defined bacterial culture (1990)
    Springer
    Current microbiology 20 (1990), S. 77-81 
    Details
    ISSN: 1432-0991
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Abstract Thermophilic degradation of pectin was studied in batch cultures at 55°C by different associations of anaerobic bacteria, includingClostridium thermocellum, Methanobacterium sp., andMethanosarcina sp.Clostridium thermocellum alone produced large amounts of methanol along with some isopropanol and H2. The inoculation ofMethanobacterium sp. in the culture did not affect the metabolism ofC. thermocellum; this demonstrates the absence of interspecies hydrogen transfer. In the presence of the methylotrophicMethanosarcina sp., methanol was reduced to methane without effect on pectin hydrolysis; a small amount of the H2 produced was also used to reduce methanol.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF02092877
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  • 9
    Electronic Resource
    Electronic Resource
    Fructose degradation byDesulfovibrio sp. in pure culture and in coculture withMethanospirillum hungatei (1986)
    Springer
    Current microbiology 13 (1986), S. 285-289 
    Details
    ISSN: 1432-0991
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Abstract In a mineral medium containing sulfate, the sulfate-reducing bacteriumDesulfovibrio sp. strain JJ degraded 1 mol of fructose stoichiometrically to 1 mol of H2S, 2 mol of acetate, and presumably 2 mol of CO2. The doubling time was 10 h, and the yield was 41.6 g dry weight/mol fructose degraded. In the absence of sulfate, the hydrogenophilic methanogenMethanospirillum hungatei replaced sulfate as hydrogen sink. In such cocultures, 1 mol of fructose was converted to acetate, methane, succinate, and presumably CO2 in varying concentrations. The growth yield of the H2-transferring association was 33 g dry weight/mol fructose. In the absence of sulfate,Desulfovibrio strain JJ slowly fermented 1 mol of fructose to 1 mol of succinate, 0.5 mol of acetate, and 0.5 mol of ethanol. The results are compared with those of other anaerobic hexose-degrading bacteria.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF01568654
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  • 10
    Electronic Resource
    Electronic Resource
    Determination of the G + C content of twoSyntrophus buswellii strains by ultracentrifugation techniques (1993)
    Springer
    Current microbiology 26 (1993), S. 185-189 
    Details
    ISSN: 1432-0991
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Abstract An anaerobic syntrophic bacterial culture degrading benzoate was isolated from a river sediment. The syntrophic organism was grown in coculture in the presence of a hydrogenotrophic strain,Desulfovibrio fructosovorans orMethanospirillum hungatei. The G+C content of the syntrophic benzoate degrader determined by density gradient ultracentrifugation was similar to that ofSyntrophus buswellii (54.3%). A method ensuring the G+C% determination of syntrophic bacteria is presented.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF01577375
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