ISSN:
1399-0047
Quelle:
Crystallography Journals Online : IUCR Backfile Archive 1948-2001
Thema:
Chemie und Pharmazie
,
Geologie und Paläontologie
,
Physik
Notizen:
A recombinant α-amylase from Bacillus stearothermophilus was found to be produced as several isoforms arising from different N-terminal processing. Some of those isoforms were purified to homogeneity and crystallized at 293 K using the hanging-drop vapour-diffusion method under the following conditions: 35 mM sodium acetate (pH 4.6), 6.25%(v/v) 2-propanol, in the presence of 1.23%(w/v) acarbose (a pseudo-oligosaccharide inhibitor) in the drop. The crystals diffracted beyond 2.0 Å resolution using synchrotron radiation at the Photon Factory, Tsukuba. They belong to the monoclinic space group P21, with unit-cell parameters a = 53.7 (2), b = 92.9 (4), c = 53.2 (2) Å, β = 109.4 (1)°.
Materialart:
Digitale Medien
URL:
http://dx.doi.org/10.1107/S0907444999015772
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