ISSN:
1574-6968
Source:
Blackwell Publishing Journal Backfiles 1879-2005
Topics:
Biology
Notes:
A system for controlled targeting of heterologous protein was developed in the food-grade bacterium Lactococcus lactis. It is composed of the L. lactis strain NZ9000 and of two broad host range expression vectors pCYT:Nuc and pSEC:Nuc for, respectively, cytoplasmic and secreted staphylococcal nuclease (Nuc) nisin-inducible production. The level of intracellular production of Nuc measured with pCYT:Nuc (3 mg l−1) is significantly lower than the one obtained with pSEC:Nuc (∼20 mg l−1). The secretion efficiency (SE) of Nuc is estimated to be ∼70%, corresponding to ∼15 mg of secreted Nuc l−1. Furthermore, we established that Nuc production continued in L. lactis 10 h after a 1-h nisin-pulse induction. This system was then used for intra- and extracellular production of a protein of therapeutical interest in L. lactis, the ovine interferon-omega (IFN-ω). The SE and the quantity of secreted active IFN-ω were evaluated respectively to be ∼70% and ∼1 mg l−1 (∼two-fold higher than the cytoplasmic form).
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1016/S0378-1097(03)00475-0
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