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  • 1
    Publication Date: 2014-12-16
    Description: Correction: The ER stress inducer DMC enhances TRAIL-induced apoptosis in glioblastomaIngrid A M van Roosmalen1,2, Carlos R Reis1,3[dagger], Rita Setroikromo1, Saravanan Yuvaraj2,4, Justin V Joseph2, Pieter G Tepper1, Frank A E Kruyt2 and Wim J Quax1*Corresponding author: Wim J Quax W.J.Quax@rug.nl[dagger]Equal contributors1 Department of Pharmaceutical Biology, Groningen Research Institute of Pharmacy, University of Groningen, Antonius Deusinglaan 1, Groningen 9713 AV, The Netherlands2 Department of Medical Oncology, University of Groningen, University Medical Center Groningen, Hanzeplein 1, Groningen 9713 GZ, The Netherlands3 Present address: Department of Cell Biology, UT Southwestern Medical Center, Dallas, TX 75390-9039, USA4 Present address: Department of Pulmonary Medicine, Erasmus Medical Center, Westzeedijk 353, Rotterdam 3015 AA, The NetherlandsCorrectionThe figure numbering in the HTML version of the original article (van Roosmalen et al. 2014) was listed incorrectly, while the PDF version was correct.Figure 1 in the HTML version is Figure 2 in PDFFigure 2 in the HTML version is Figure 3 in PDFFigure 3 in the HTML version is Figure 4 in PDFFigure 4 in the HTML version is Figure 5 in PDFFigure 5 in the HTML version is Figure 6 in PDFFigure 6 in the HTML version is Figure 7 in PDFFigure 7 in the HTML version is a duplicate of additional Figure S3.The publisher would like to apologise for this error.Referencesvan Roosmalen IAM, Reis CR, Setroikromo R, Yuvaraj S, Joseph JV, Tepper PG, Kruyt FAE, Quax WJ (2014) The ER stress inducer DMC enhances TRAIL-induced apoptosis in glioblastoma. SpringerPlus 3(1):495
    Electronic ISSN: 2193-1801
    Topics: Natural Sciences in General
    Published by SpringerOpen
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  • 2
    Publication Date: 2014-09-02
    Description: Glioblastoma multiforme (GBM) is the most aggressive malignant brain tumour in humans and is highly resistant to current treatment modalities. We have explored the combined treatment of the endoplasmic reticulum (ER) stress-inducing agent 2,5-dimethyl-celecoxib (DMC) and TNF-related apoptosis-inducing ligand (TRAIL-WT) or the DR5-specific TRAIL D269H/E195R variant as a potential new strategy to eradicate GBM cells using TRAIL-resistant and -sensitive GBM cells. GBM cell lines were investigated for their sensitivity to TRAIL, DMC and combination of both agents. Cell viability was measured by MTS assay and apoptosis was assessed by Annexin V/PI and acridine orange staining. Caspase activation and protein expression levels were analysed with Western blotting. Death Receptor (DR) cell surface expression levels were quantified by flow cytometry. DR5 expression was increased in U87 cells by ectopic expression using a retroviral plasmid and survivin expression was silenced using specific siRNAs. We demonstrate that A172 expresses mainly DR5 on the cell surface and that these cells show increased sensitivity for the DR5-specific rhTRAIL D269H/E195R variant. In contrast, U87 cells show low DR cell surface levels and is insensitive via both DR4 and DR5. We determined that DMC treatment displays a dose-dependent reduction in cell viability against a number of GBM cells, associated with ER stress induction, as shown by the up-regulation of glucose-regulated protein 78 (GRP78) and CCAAT/-enhancer-binding protein homologous protein (CHOP) in A172 and U87 cells. The dramatic decrease in cell viability is not accompanied by a correspondent increase in Annexin V/PI or caspase activation typically seen in apoptotic or/and necrotic cells within 24h of treatment. Although DMC did not affect DR5 expression in the GBM cells, it increased TRAIL-induced caspase-8 activation in both TRAIL-sensitive and -resistant cells, indicating that DMC potentiates initiator caspase activation in these cells. In A172 cells, sub-toxic concentrations of DMC greatly potentiated TRAIL-induced apoptosis. Furthermore, DMC strongly reduced survivin expression in A172 and U87 cells and silencing of this anti-apoptotic protein partially sensitized cells to TRAIL-induced apoptosis. Our findings corroborate that DMC is a promising agent against GBM, and uncovers a potential synergistic cooperation with TRAIL in this highly malignant cancer.
    Electronic ISSN: 2193-1801
    Topics: Natural Sciences in General
    Published by SpringerOpen
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  • 3
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    Coimbra University Press
    Publication Date: 2022-01-31
    Language: Portuguese
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  • 4
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    Coimbra University Press
    Publication Date: 2022-01-31
    Description: Encontram-se reunidos neste livro ensaios diretamente relacionados com o projeto de investigação “Figuras da Ficção” (Centro de Literatura Portuguesa da Faculdade de Letras de Coimbra), com extensão ao blogue homónimo (www.figurasdaficcao.wordpress.com). Centrando-se na personagem, nos seus modos de existência ficcional e paraficcional, nas suas figurações e nos seus avatares, os ensaios que aqui se encontram tratam de revalorizar uma categoria narrativa que durante décadas foi relegada para as margens dos estudos literários.〈/p〉Nos últimos vinte anos, os estudos narrativos, tendo colhido muitas das conquistas conceptuais e metodológicas da narratologia dos anos 80 do século passado, redescobriram na personagem um apreciável potencial de investimento semântico, de dinamismo transficcional e de articulação intercultural. É isso que está confirmado nos estudos em que aqui são tratados temas e subtemas como a figuração da personagem realista, a refiguração de personalidades históricas, a questão do insólito ou a representação paraficcional de figuras do chamado universo mediático.
    Language: Portuguese
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  • 5
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    Coimbra University Press
    Publication Date: 2022-01-31
    Description: O Congresso Internacional “José Saramago: 20 Anos do Prémio Nobel” foi uma oportunidade privilegiada para se pensar e debater a obra de um grande escritor. Durante os três dias em que teve lugar, o congresso permitiu atualizar conhecimentos e abrir caminhos de reflexão sobre praticamente todos os aspetos da vasta e multifacetada obra do escritor: os seus romances e os grandes temas que neles estão representados, as personagens e os seus modos de existência, a poesia e o teatro, a cronística e as adaptações da ficção a outras artes foram objeto de cerca de cinco dezenas de comunicações, da autoria de participantes oriundos de vários países, com destaque para Portugal e o Brasil.
    Keywords: characters ; chronicle writing ; theater ; José Saramago ; adaptation ; allegory ; critical wealth ; novels ; journalism
    Language: Portuguese
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  • 6
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    Coimbra University Press
    Publication Date: 2023-12-20
    Keywords: PN1-6790 ; Portuguese ; bic Book Industry Communication::D Literature & literary studies
    Language: Portuguese
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  • 7
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    Coimbra University Press
    Publication Date: 2022-01-31
    Description: This work brings together the production of researchers integrated in the project "Figures of Fiction", as well as other papers on the theme of this issue: "Dynamics of the Character". This theme focus on reflections on the transliterary vitality of the character and the movements of refiguration that it motivates. Therefore, the work concerns the literature, in several times and narrative genres, the cinema, the arts, the television, the publicity, the media speeches and the videogames, stressing the dynamism of the character, as a key category of the narrative
    Keywords: Metalepse ; Narrative genres ; Afterlife ; Character ; Figuration
    Language: Portuguese
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  • 8
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    Coimbra University Press
    Publication Date: 2023-12-20
    Description: Encontram-se reunidos neste livro ensaios diretamente relacionados com o projeto de investigação “Figuras da Ficção” (Centro de Literatura Portuguesa da Faculdade de Letras de Coimbra), com extensão ao blogue homónimo (www.figurasdaficcao.wordpress.com). Centrando-se na personagem, nos seus modos de existência ficcional e paraficcional, nas suas figurações e nos seus avatares, os ensaios que aqui se encontram tratam de revalorizar uma categoria narrativa que durante décadas foi relegada para as margens dos estudos literários. Nos últimos vinte anos, os estudos narrativos, tendo colhido muitas das conquistas conceptuais e metodológicas da narratologia dos anos 80 do século passado, redescobriram na personagem um apreciável potencial de investimento semântico, de dinamismo transficcional e de articulação intercultural. É isso que está confirmado nos estudos em que aqui são tratados temas e subtemas como a figuração da personagem realista, a refiguração de personalidades históricas, a questão do insólito ou a representação paraficcional de figuras do chamado universo mediático.〈br /〉
    Keywords: PN1-6790 ; Portuguese ; bic Book Industry Communication::D Literature & literary studies
    Language: Portuguese
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  • 9
    Publication Date: 2017-01-03
    Description: Clathrin-mediated endocytosis (CME) constitutes the major pathway for uptake of signaling receptors into eukaryotic cells. As such, CME regulates signaling from cell-surface receptors, but whether and how specific signaling receptors reciprocally regulate the CME machinery remains an open question. Although best studied for its role in membrane fission, the GTPase dynamin also regulates early stages of CME. We recently reported that dynamin-1 (Dyn1), previously assumed to be neuron-specific, can be selectively activated in cancer cells to alter endocytic trafficking. Here we report that dynamin isoforms differentially regulate the endocytosis and apoptotic signaling downstream of TNF-related apoptosis-inducing ligand–death receptor (TRAIL–DR) complexes in several cancer cells. Whereas the CME of constitutively internalized transferrin receptors is mainly dependent on the ubiquitously expressed Dyn2, TRAIL-induced DR endocytosis is selectively regulated by activation of Dyn1. We show that TRAIL stimulation activates ryanodine receptor-mediated calcium release from endoplasmic reticulum stores, leading to calcineurin-mediated dephosphorylation and activation of Dyn1, TRAIL–DR endocytosis, and increased resistance to TRAIL-induced apoptosis. TRAIL–DR-mediated ryanodine receptor activation and endocytosis is dependent on early caspase-8 activation. These findings delineate specific mechanisms for the reciprocal crosstalk between signaling and the regulation of CME, leading to autoregulation of endocytosis and signaling downstream of surface receptors.
    Print ISSN: 0027-8424
    Electronic ISSN: 1091-6490
    Topics: Biology , Medicine , Natural Sciences in General
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  • 10
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