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Enzyme-linked immunosorbent assay (ELISA) in the paracoccidioidomycosis (1984)

Camargo, Z. Pires ; Guesdon, J. L. ; Drouhet, E. ; [et al.]

Springer

Mycopathologia 88 (1984), S. 31-37

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ISSN: 1573-0832

Source: Springer Online Journal Archives 1860-2000

Topics: Biology , Medicine

Notes: Abstract An enzyme-linked immunosorbent assay (ELISA) for detection and quantification of antibodies antiParacoccidioides brasiliensis is described. Polystyrene plates have been used as solid phase to absorb P. brasiliensis metabolic yeast phase antigen. Twenty sera of proven paracoccidioidomycosis, 11 of histoplasmosis due Histoplasma capsulatum, 20 of aspergillosis and 20 human normal sera were tested. Ninety-five percent of the paracoccidioidomycosis sera had O.D. superior to 0.150 (from 0.163 to 2.650) at 1/400 serum dilution. ELISA assay was compared with counterimmunoelectrophoresis and erythro-immunoassay tests; a correlation was observed only with erythro-immunoassay. ELISA test should give new perspectives for the serodiagnosis of paracoccidioidomycosis.

Type of Medium: Electronic Resource

URL: http://dx.doi.org/10.1007/BF00439292

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