ISSN:
1573-5036
Keywords:
Nitrate reductase
;
Nitrate reduction
;
Nitrate reduction
;
StoichiometryZea mays L.
Source:
Springer Online Journal Archives 1860-2000
Topics:
Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
Notes:
Summary Reported non-stoichiometry in the reduction of nitrate to nitrite was investigated in leaves ofZea mays L.. The nitrogen balance sheet forin vitro nitrate assimilation was influenced by enzyme protectants in the extraction media and by the method employed to terminate the reaction. A number of limitations were found in the generally acceptedin vitro nitrate reductase assay, in particular the presence of endogenous components which interfered with the assay of nitrite were considered. A stoichiometric balance for thein vitro reduction of nitrate to nitrite was obtained when interfering factors were minimized. The absence of back reactions from ammonia in the assay was confirmed.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1007/BF02198118
Permalink