Publication Date:
2012-01-28
Description:
To estimate the number and diversity of beneficial mutations, we experimentally evolved 115 populations of Escherichia coli to 42.2 degrees C for 2000 generations and sequenced one genome from each population. We identified 1331 total mutations, affecting more than 600 different sites. Few mutations were shared among replicates, but a strong pattern of convergence emerged at the level of genes, operons, and functional complexes. Our experiment uncovered a set of primary functional targets of high temperature, but we estimate that many other beneficial mutations could contribute to similar adaptive outcomes. We inferred the pervasive presence of epistasis among beneficial mutations, which shaped adaptive trajectories into at least two distinct pathways involving mutations either in the RNA polymerase complex or the termination factor rho.〈br /〉〈span class="detail_caption"〉Notes: 〈/span〉Tenaillon, Olivier -- Rodriguez-Verdugo, Alejandra -- Gaut, Rebecca L -- McDonald, Pamela -- Bennett, Albert F -- Long, Anthony D -- Gaut, Brandon S -- New York, N.Y. -- Science. 2012 Jan 27;335(6067):457-61. doi: 10.1126/science.1212986.〈br /〉〈span class="detail_caption"〉Author address: 〈/span〉Department of Ecology and Evolutionary Biology, University of California-Irvine, CA 92697, USA. olivier.tenaillon@inserm.fr〈br /〉〈span class="detail_caption"〉Record origin:〈/span〉 〈a href="http://www.ncbi.nlm.nih.gov/pubmed/22282810" target="_blank"〉PubMed〈/a〉
Keywords:
*Adaptation, Physiological
;
DNA-Directed RNA Polymerases/genetics
;
Epistasis, Genetic
;
Escherichia coli/*genetics/*physiology
;
*Evolution, Molecular
;
Genes, Bacterial
;
Genetic Fitness
;
Genome, Bacterial
;
Models, Genetic
;
*Mutation
;
Operon
;
Point Mutation
;
Rho Factor/genetics
;
Selection, Genetic
;
Sequence Analysis, DNA
;
Sequence Deletion
;
Temperature
Print ISSN:
0036-8075
Electronic ISSN:
1095-9203
Topics:
Biology
,
Chemistry and Pharmacology
,
Computer Science
,
Medicine
,
Natural Sciences in General
,
Physics
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