ISSN:
1662-8985
Quelle:
Scientific.Net: Materials Science & Technology / Trans Tech Publications Archiv 1984-2008
Thema:
Maschinenbau
Notizen:
Bacterial photosynthetic antenna complexes are composed of α-helical hydrophobicpolypeptides and pigments (e.g., bacteriochlorophyll a). We report here self-assembling properties ofan engineered hydrophobic polypeptide with zinc-substituted bacteriochlorophyll a ([Zn]-BChl a) invarious lipid bilayer to investigate the effect of lipid species on the self-assembling properties. Whenthe polypeptide and [Zn]-BChl a were mixed in surfactant solution (n-octyl β-D-glucopyranoside:OG) at 25°C, the absorption band [Zn]-BChl a was red-shifted from 770 to 812 nm, that is assignableto quasi-dimeric “subunit-type” complex. By subsequent dilution and cooling of the solution, theabsorption band further red-shifted to 836 nm indicative for progressed assembly, ‘LH1-type’complex. When the subunit-type complex was assembled in lipid bilayer, e.g., phosphatidylcholines(PC’s) and phosphatidylglycerol (PG’s), the assembling property to form LH1-type complexprominently depended on the character of their fatty acid chains in the phospholipids. The subunitcomplex remained in the fluidic bilayers, but further assembled to form LH1-type complex insolid-like phospholipid bilayers, suggesting that intermolecular force of phospholipid governs theassembling of the polypeptide/[Zn]-BChl a complex
Materialart:
Digitale Medien
URL:
http://www.tib-hannover.de/fulltexts/2011/0528/01/39/transtech_doi~10.4028%252Fwww.scientific.net%252FAMR.11-12.623.pdf
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