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  • 1
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    Determination of developmental stages of embryo in the Sea Urchin, Echinometra mathaei (2018)
    Details
    Publikationsdatum: 2021-05-19
    Beschreibung: Sea Urchin is one of the most useful tools in developmental biology studies because this organism has the simplest kind of developmental stages. We aimed to determine developmental stages and timetable of Echinometra mathaei embryo (the species of Persian Gulf). The spawning of E. mathaei was induced by 0.5M KCl injection (1ml) into the coelomic cavity. After fertilization, embryos were placed in beakers and were incubated at 29◦C and a salinity of 39 ppt until embryos reached the pluteus stage. The developmental stages of embryos and the timing of each stage including cleavage, morulae, blastula, gastrula, prism and pluteus larvae were studied under the microscope. Our results showed that after 30 hours from fertilization time, the embryos developed to pluteus larvae. E. mathaei had the shorter development time in comparison to the other Sea Urchin species. Therefore, it may be appropriate as a model organism in biological researches.
    Beschreibung: Published
    Schlagwort(e): Determination ; Sea Urchin ; Echinometra mathaei ; Biological Model ; Development ; Timetable ; Species
    Repository-Name: AquaDocs
    Materialart: Journal Contribution , Refereed
    Format: pp.294-304
    Standort Signatur Erwartet Verfügbarkeit
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    ARTIKEL (OA)
  • 2
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    Effects of mercury on embryonic development and larval growth of the sea urchin Echinometra mathaei from the Persian Gulf (2018)
    Details
    Publikationsdatum: 2021-05-19
    Beschreibung: This study investigated the effects of increasing mercury (Hg) concentration on early developmental stages of sea urchin, Echinomethra mathaei, as a bioindicator. The toxicity test was carried out after the gamete released induction and fertilization in six concentrations of mercury within the range of 4, 8, 16, 32, 64 and 128 µg/L. Embryos samples were incubated for 30 h in control and test solutions. After incubation, the percentage of developed 4-arm pluteus larvae was recorded in each group and embryonic abnormalities were studied by a microscope. Results of this study indicate that exposure of embryos to increasing mercury concentrations lead to abnormalities such as changes to shape and size of pluteus larval arms and also arrested development in early embryonic stages. Furthermore, embryos were analyzed to determine mercury absorption by cold vapour atomic absorption spectrometry method (AAS). The median effective concentration (EC50) value calculated for mercury was 17/42 µg/L.
    Beschreibung: Published
    Schlagwort(e): Biology ; Mercury ; Sea urchin ; Toxicity test ; Echinometra mathaei ; Larval ; Growth
    Repository-Name: AquaDocs
    Materialart: Journal Contribution , Refereed
    Format: pp.898-907
    Standort Signatur Erwartet Verfügbarkeit
    BibTip Andere fanden auch interessant ...
    ARTIKEL (OA)
  • 3
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    Effects of mercury on embryonic development and larval growth of the sea urchin Echinometra mathaei from the Persian Gulf (2021)
    In:  http://aquaticcommons.org/id/eprint/22656 | 18721 | 2018-05-09 21:05:27 | 22656 | Iranian Fisheries Science Research Institute
    Details
    Publikationsdatum: 2021-07-08
    Beschreibung: This study investigated the effects of increasing mercury (Hg) concentration on early developmental stages of sea urchin, Echinometra mathaei, as a bioindicator. The toxicity test was carried out after the gamete released induction and fertilization in six concentrations of mercury within the range of 4, 8, 16, 32, 64 and 128 µg/L. Embryos samples were incubated for 30 h in control and test solutions. After incubation, the percentage of developed 4-arm pluteus larvae was recorded in each group and embryonic abnormalities were studied by a microscope. Results of this study indicate that exposure of embryos to increasing mercury concentrations lead to abnormalities such as changes to shape and size of pluteus larval arms and also arrested development in early embryonic stages. Furthermore, embryos were analyzed to determine mercury absorption by cold vapour atomic absorption spectrometry method (AAS). The median effective concentration (EC50) value calculated for mercury was 17/42 µg/L.
    Schlagwort(e): Biology ; Environment ; Pollution ; Mercury ; Sea urchin ; Toxicity test ; Echinometra mathaei ; Persian Gulf ; Iran
    Repository-Name: AquaDocs
    Materialart: article , TRUE
    Format: application/pdf
    Format: application/pdf
    Format: 898-907
    Standort Signatur Erwartet Verfügbarkeit
    BibTip Andere fanden auch interessant ...
    ARTIKEL (OA)
  • 4
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    Determination of developmental stages of embryo in the sea urchin, Echinometra mathaei (2021)
    In:  http://aquaticcommons.org/id/eprint/22512 | 18721 | 2018-04-16 21:02:07 | 22512 | Iranian Fisheries Science Research Institute
    Details
    Publikationsdatum: 2021-07-06
    Beschreibung: Sea Urchin is one of the most useful tools in developmental biology studies because this organism has the simplest kind of developmental stages. We aimed to determine developmental stages and timetable of Echinometra mathaei embryo (the species of Persian Gulf). The spawning of E. mathaei was induced by 0.5M KCl injection (1ml) into the coelomic cavity. After fertilization, embryos were placed in beakers and were incubated at 29°C and a salinity of 39 ppt until embryos reached the pluteus stage. The developmental stages of embryos and the timing of each stage including cleavage, morulae, blastula, gastrula, prism and pluteus larvae were studied under the microscope. Our results showed that after 30 hours from fertilization time, the embryos developed to pluteus larvae. E. mathaei had the shorter development time in comparison to the other Sea Urchin species. Therefore, it may be appropriate as a model organism in biological researches.
    Schlagwort(e): Biology ; Sea Urchin ; Echinometra mathaei ; Biological Model ; Development ; Timetable ; Persian Gulf ; Iran
    Repository-Name: AquaDocs
    Materialart: article , TRUE
    Format: application/pdf
    Format: application/pdf
    Format: 294-304
    Standort Signatur Erwartet Verfügbarkeit
    BibTip Andere fanden auch interessant ...
    ARTIKEL (OA)
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