ISSN:
1052-9306
Schlagwort(e):
Chemistry
;
Analytical Chemistry and Spectroscopy
Quelle:
Wiley InterScience Backfile Collection 1832-2000
Thema:
Chemie und Pharmazie
Notizen:
A stable isotope dilution assay has been developed for the quantitative determination of cholesterol sulphate in plasma using negative ion fast atom bomardment (FAB) mass spectrometry. The assay is highly selective and avoids problems of contamination from free cholesterol and other conjugates of cholesterol present in plasma. (6,7,7-2H3)Cholesterol sulphate is used as the internal standard and solvent extraction and silica Sep-Paks are employed to isolate plasma cholesterol sulphate. Limited-range acceleration voltage scanning in FAB mass spectrometric analyses leads to sub-microgram detection limits. Comparison of results obtained by FAB mass spectrometry of the intact cholesterol sulphate, and by gas chromatography/mass spectrometry selected ion monitoring of the free cholesterol, released by solvolysis of the cholesterol sulphate, showed that the latter approach probably overestimates plasma levels of cholesterol sulphate.
Zusätzliches Material:
4 Ill.
Materialart:
Digitale Medien
URL:
http://dx.doi.org/10.1002/bms.1200191002
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