ALBERT — All Library Books, journals and Electronic Records Telegrafenberg

Digitale Medien

A fiber-bed bioreactor for anchorage-dependent animal cell cultures: Part II. Scaleup potential (1991)

Murakami, Sei ; Chiou, Tzyy-Wen ; Wang, Daniel I. C.

New York, NY [u.a.] : Wiley-Blackwell

Biotechnology and Bioengineering 37 (1991), S. 762-769

zur Merkliste hinzufügen auf der Merkliste

Details

ISSN: 0006-3592

Schlagwort(e): animal cell culture ; bioreactor ; airlift ; fiber-bed bioreactor ; oxygen transfer ; Chemistry ; Biochemistry and Biotechnology

Quelle: Wiley InterScience Backfile Collection 1832-2000

Thema: Biologie , Werkstoffwissenschaften, Fertigungsverfahren, Fertigung

Notizen: A simple hydrodynamic model is introduced to describe the airlift fiber-bed bioreactor, which can enhance the volumetric productivity of anchorage-dependent animal cell cultures. By applying the model, liquid flow rates and volumetric mass transfer coefficients are predicted and are in agreement with experimental measurements. Consequently, the optimal reactor configuration giving the maximal oxygen supply is derived. Also, theoretical scaleup potential of this concentric internal loop reactor is considered for volumes ranging from 10 to 67,000 L with which cell densities of 5.1 × 107 and 1.2 × 107 cells/cm3, respectively, can be maintained.

Zusätzliches Material: 8 Ill.

Materialart: Digitale Medien

URL: http://dx.doi.org/10.1002/bit.260370811

Permalink

	Standort	Signatur	Erwartet	Verfügbarkeit

Andere fanden auch interessant ...

Artikel (Nationallizenzen)

Volltext

Digitale Medien

A fiber-bed bioreactor for anchorage-dependent animal cell cultures: Part I. Bioreactor design and operations (1991)

Chiou, Tzyy-Wen ; Murakami, Sei ; Wang, Daniel I. C. ; [weitere]

New York, NY [u.a.] : Wiley-Blackwell

Biotechnology and Bioengineering 37 (1991), S. 755-761

zur Merkliste hinzufügen auf der Merkliste

Details

ISSN: 0006-3592

Schlagwort(e): cell culture ; fiber-bed bioreactor ; anchorage-dependent cell cultures ; airlift ; bioreactor design ; Chemistry ; Biochemistry and Biotechnology

Quelle: Wiley InterScience Backfile Collection 1832-2000

Thema: Biologie , Werkstoffwissenschaften, Fertigungsverfahren, Fertigung

Notizen: A concentric-cylinder airlift reactor, in which the annulus is a packed bed of glass fibers, has been developed in order to facilitate the scaleup and enhance the volumetric productivity of anchorage-dependent animal cell cultures. In this bio-reactor, oxygen-containing gas is sparged through the inner draft tube, causing bubble-free medium to flow through the fiber bed in the outer cylinder and providing both oxygenation and convective nutrient transfer to the cells. Several other desirable features for reactor operation are also provided by this design. Cell cultivations in this bioreactor have been successfully carried out and provide data for the feasibility of the large-scale cell cultivation.

Zusätzliches Material: 8 Ill.

Materialart: Digitale Medien

URL: http://dx.doi.org/10.1002/bit.260370810

Permalink

	Standort	Signatur	Erwartet	Verfügbarkeit

Andere fanden auch interessant ...

Artikel (Nationallizenzen)

Volltext

Digitale Medien

Automated monitoring of cell concentration and viability using an image analysis system (1994)

Maruhashi, Fumio ; Murakami, Sei ; Baba, Kenji

Springer

Cytotechnology 15 (1994), S. 281-289

zur Merkliste hinzufügen auf der Merkliste

Details

ISSN: 1573-0778

Schlagwort(e): Animal cell culture ; cell debris ; cell viability ; image analysis

Quelle: Springer Online Journal Archives 1860-2000

Thema: Biologie , Medizin , Werkstoffwissenschaften, Fertigungsverfahren, Fertigung

Notizen: Abstract In order to automate measurements of cell concentration and viability in a suspended animal cell culture, we have developed anin situ microscopic image analysis system with an effective cell recognition algorithm. With a small amount of sample, this system can measure the cell density rapidly and aseptically. In addition, it can measure a cell size histogram including cell debris small particle distribution. These small particles have been found to be related to the viability of the mouse-mouse hybridoma STK1 cell line. By using cell debris small particle density as an indicator of cell viability, the developed system provides non-destructive viability monitoring without trypan blue staining.

Materialart: Digitale Medien

URL: http://dx.doi.org/10.1007/BF00762403

Permalink

	Standort	Signatur	Erwartet	Verfügbarkeit

Andere fanden auch interessant ...

Artikel (Nationallizenzen)

Volltext

Unbekannt

A gain-of-function screen to identify genes that reduce lifespan in the adult of Drosophila melanogaster (2014)

Minoru Nakayama; Tomoki Ishibashi; Hiroyuki Ishikawa; Hiroyasu Sato; Takao Usui; Takayuki Okuda; Hiroyuki Yashiro; Hironori Ishikawa; Yoshie Taikou; Asako Minami; Kengo Kato; Masataka Taki; Toshiro Aigaki; Wataru Gunji; Masaya Ohtsu; Yasufumi Murakami; Sei-ichi Tanuma; Alice Tsuboi; Mai Adachi; Junpei Kuroda; Takeshi Sasamura; Tomoko Yamakawa; Kenji Matsuno

BioMed Central

In: BMC Genetics

zur Merkliste hinzufügen auf der Merkliste

Details

Publikationsdatum: 2014-04-17

Beschreibung: Background: Several lines of evidence associate misregulated genetic expression with risk factors for diabetes, Alzheimer's, and other diseases that sporadically develop in healthy adults with no background of hereditary disorders. Thus, we are interested in genes that may be expressed normally through parts of an individual's life, but can cause physiological defects and disease when misexpressed in adulthood. Results: We attempted to identify these genes in a model organism by arbitrarily misexpressing specific genes in adult Drosophila melanogaster, using 14,133 Gene Search lines. We identified 39 "reduced-lifespan genes" that, when misexpressed in adulthood, shortened the flies' lifespan to less than 30% of that of control flies. About half of these genes have human orthologs that are known to be involved in human diseases. For about one-fourth of the reduced-lifespan genes, suppressing apoptosis restored the lifespan shortened by their misexpression. We determined the organs responsible for reduced lifespan when these genes were misexpressed specifically in adulthood, and found that while some genes induced reduced lifespan only when misexpressed in specific adult organs, others could induce reduced lifespan when misexpressed in various organs. This finding suggests that tissue-specific dysfunction may be involved in reduced lifespan related to gene misexpression. Gene ontology analysis showed that reduced-lifespan genes are biased toward genes related to development. Conclusions: We identified 39 genes that, when misexpressed in adulthood, shortened the lifespan of adult flies. Suppressing apoptosis rescued this shortened lifespan for only a subset of the reduced-lifespan genes. The adult tissues in which gene misexpression caused early death differed among the reduced-lifespan genes. These results suggest that the cause of reduced lifespan upon misexpression differed among the genes.

Digitale ISSN: 1471-2156

Thema: Biologie