ISSN:
1432-0614
Source:
Springer Online Journal Archives 1860-2000
Topics:
Biology
,
Process Engineering, Biotechnology, Nutrition Technology
Notes:
Summary An NTG induced mutant of a bile acid utilizing strain of Pseudomonas putida was isolated which was blocked in the steroid catabolic pathway. This mutant (PS5-7) was able to accumulate both phenolic and catecholic secosteroid products from cholic acid. Using a transposing system containing the kanamycin resistance transposon Tn5, mutants of PS5-7 were isolated which produced only the phenolic secosteroid. One of these mutants (PS5-25) was able to produce close to theoretical yields of various phenolic secosteroids from corresponding bile acids.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1007/BF00286315
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