ISSN:
1399-0047
Source:
Crystallography Journals Online : IUCR Backfile Archive 1948-2001
Topics:
Chemistry and Pharmacology
,
Geosciences
,
Physics
Notes:
A highly thermostable DNA ligase from Thermus filiformis has been crystallized at room temperature using methoxypolyethylene glycol 5000 as a precipitant. The crystal belongs to the monoclinic space group P21, with unit-cell parameters a = 90.63, b = 117.80, c = 98.65 Å, β = 115.56°. Two molecules of DNA ligase are present in the asymmetric unit, giving a crystal volume per protein mass (Vm) of 3.1 Å3 Da−1 and a solvent content of 61%. A native data set extending to 3.0 Å resolution has been collected at 100 K using synchrotron X-rays.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1107/S090744490000010X
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