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  • 1
    Electronic Resource
    Electronic Resource
    Palo Alto, Calif. : Annual Reviews
    Annual Review of Phytopathology 36 (1998), S. 27-40 
    ISSN: 0066-4286
    Source: Annual Reviews Electronic Back Volume Collection 1932-2001ff
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition , Biology
    Type of Medium: Electronic Resource
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  • 2
    ISSN: 0931-1890
    Keywords: Key words Cell division ; Cytokinesis ; Fusiform cells ; Mitosis ; Robinia pseudoacacia
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Abstract  The ultrastructure of periclinally dividing fusiform cells was studied in the vascular cambium of Robinia pseudoacacia. Fusiform cell division begins in April at Madison, Wisconsin, when the cambial cells still have many characteristics of a dormant cambium. Soon afterward, the cambial cells acquire the appearance typical of an active cambium. Sequential phases of the microtubule cycle were documented: cortical microtubules bordering the cell wall during interphase, perinuclear microtubules preceding formation of the mitotic spindle, spindle microtubules, and phragmoplast microtubules. A preprophase band of microtubules was not encountered. An extended phragmosome was not encountered in periclinally dividing fusiform cells. During cytokinesis, the phragmosome is represented by a broad cytoplasmic plate which precedes the developing phragmoplast and cell plate as they migrate toward the ends of the cell.
    Type of Medium: Electronic Resource
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  • 3
    Electronic Resource
    Electronic Resource
    Springer
    Trees 11 (1997), S. 191-202 
    ISSN: 0931-1890
    Keywords: Key words Endoplasmic reticulum ; Robinia pseudoacacia ; Storage materials ; Vacuole ; Vascular cambium ultrastructure
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Abstract  The ultrastructure of the vascular cambium of Robinia pseudoacacia L. was examined in trunk tissues collected over a 2 1/2 year period. During dormancy, fusiform cells are densely cytoplasmic with many small vacuoles and centrally located nuclei. Mitochondria are round to oval in sectional view. The plastids are variable in shape, have few internal membranes, and generally lack starch grains. The plasmalemma is smooth in outline. Proteinaceous material occurs in the vacuoles and many lipid droplets are scattered throughout the ground substance. Smooth tubular ER, often highly dilated, predominates, but short segments of rough ER are also present. Abundant free ribosomes are evenly distributed throughout the ground substance and the dictyosomes are inactive. Microtubules are parietal and have various orientations. During reactivation, the plasmalemma becomes irregular in outline and begins to form invaginations. Concurrently, the proteinaceous material disappears, the vacuoles begin to fuse, polysomes appear, and the dictyosomes begin to produce vesicles. During the period of cambial activity, fusiform cells are highly vacuolate, and the nuclei are centrally located. The mitochondria are round, oval, or elongate. Now the plastids contain phytoferritin, starch grains, or both. Many large invaginations of the plasmalemma intrude into the vacuole, pushing the tonoplast inward and pinching off into the vacuole, which lacks proteinaceous material. Lipid droplets are scarce. Most ER is rough, and ribosomes are generally aggregated as polysomes. Dictyosomes are actively producing vesicles. During the transition to dormancy, the fusiform cells gradually assume the appearance typical of the dormant cambium.
    Type of Medium: Electronic Resource
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  • 4
    Electronic Resource
    Electronic Resource
    Springer
    Planta 100 (1971), S. 208-221 
    ISSN: 1432-2048
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary Sieve-tube exudate which appears on cut surfaces of stems of Cucurbita maxima as distinct droplets has been depicted in electron micrographs of longitudinal sections of the phloem. The exudate, which was produced from mature sieve tubes only, contained filaments of P-protein, but no mitochondria or vesicles of endoplasmic reticulum. The water-soluble part of the exudate contained at least 12 proteins, as shown by disc-electrophoresis. Enzymic activity was found for peroxidases, acid phosphatases, and aldolases. Color tests and assays for other enzymes, including ATPase, fructokinase, several dehydrogenases, and UDP-glucose: D-fructose-2-glucosyl transferase, gave negative results. With repeated cutting of a stem, the protein content of the exudate increased, while the amount of exudate decreased.
    Type of Medium: Electronic Resource
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  • 5
    ISSN: 1432-2048
    Keywords: Assimilate transport ; Leaf (14C transport) ; Phloem loading ; Sieve tube ; Vascular bundle ; Zea (14C transport)
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Microautoradiographs showed that [14C]sucrose taken up in the xylem of small and intermediate (longitudinal) vascular bundles of Zea mays leaf strips was quickly accumulated by vascular parenchyma cells abutting the vessels. The first sieve tubes to exhibit 14C-labeling during the [14C]sucrose experiments were thick-walled sieve tubes contiguous to the more heavily labeled vascular parenchyma cells. (These two cell types typically have numerous plasmodesmatal connections.) With increasing [14C]sucrose feeding periods, greater proportions of thick- and thin-walled sieve tubes became labeled, but few of the labeled thin-walled sieve tubes were associated with labeled companion cells. (Only the thin-walled sieve tubes are associated with companion cells.) When portions of leaf strips were exposed to 14CO2 for 5 min, the vascular parenchyma cells-regardless of their location in relation to the vessels or sieve tubes-were the most consistently labeled cells of small and intermediate bundles, and label (14C-photosynthate) appeared in a greater proportion of thin-walled sieve tubes than thick-walled sieve tubes. After a 5-min chase with 12CO2, the thin-walled sieve tubes were more heavily labeled than any other cell type of the leaf. After a 10-min chase with 12CO2, the thin-walled sieve tubes were even more heavily labeled. The companion cells generally were less heavily labeled than their associated thin-walled sieve tubes. Although all of the thick-walled sieve tubes were labeled in portions of leaf strips fed 14CO2 for 5 min and given a 10-min 12CO2 chase, only five of 72 vascular bundles below the 14CO2-exposed portions contained labeled thick-walled sieve tubes. Moreover, the few labeled thick-walledsieve tubes of the “transport region” always abutted 14C-labeled vascular parenchyma cells. The results of this study indicate that (1) the vascular parenchyma cells are able to retrieve at least sucrose from the vessels and transfer it to the thick-walled sieve tubes, (2) the thick-walled sieve tubes are not involved in long-distance transport, and (3) the thin-walled sieve tubes are capable themselves of accumulating sucrose and photosynthates from the apoplast, without the companion cells serving as intermediary cells.
    Type of Medium: Electronic Resource
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  • 6
    Electronic Resource
    Electronic Resource
    Springer
    Planta 177 (1989), S. 24-34 
    ISSN: 1432-2048
    Keywords: Brush border ; Cell wall ; Hordeum (cell wall-plasmalemma interface) ; Leaf veins ; Plasmalemma ; Sieve tubes
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Both thick- and thin-walled sieve tubes in leaf-blade veins of Hordeum vulgare L. exhibit a distinct, electron-opaque inner wall layer after fixation in glutaraldehyde-osmium tetroxide and staining with uranyl acetate and lead citrate. This inner wall layer is thickest at the sieve plates and lateral sieve areas where it is permeated by a labyrinth of tubules formed by the plasmalemma. Along the lateral walls between sieve areas the inner wall layer apparently is penetrated by numerous microvilli-like evaginations of the plasmalemma, giving the cell wall-plasmalemma interface the appearance of a brush border. It is suggested that a similar brush-border-like structure may occur at the cell wall-plasmalemma interface of sieve elements in a wide variety of vascular plants.
    Type of Medium: Electronic Resource
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  • 7
    Electronic Resource
    Electronic Resource
    Springer
    Planta 100 (1971), S. 262-267 
    ISSN: 1432-2048
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary The sieve-plate pores of sieve elements in leaf veins of Hordeum vulgare, fixed in glutaraldehyde with postfixation in osmium tetroxide, were lined by the plasmalemma and variable amounts of callose. All pores were filled with endoplasmic reticulum, which was continuous from cell to cell. Mature sieve elements lacked P-protein.
    Type of Medium: Electronic Resource
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  • 8
    Electronic Resource
    Electronic Resource
    Springer
    Planta 65 (1965), S. 180-185 
    ISSN: 1432-2048
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary Acid phosphatases are localized on the internal strands of sieve-tube members in the secondary phloem of Tilia americana. Companion cells and certain strand parenchyma cells associated with sieve-tube members show a high activity of acid phosphatases.
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  • 9
    Electronic Resource
    Electronic Resource
    Springer
    Planta 73 (1967), S. 301-318 
    ISSN: 1432-2048
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary Parenchyma cells of the secondary phloem in Pinus strobus have all the cellular organelles common in other plant cells. They have mitochondria, endoplasmic reticulum, ribosomes, dictyosomes, and plastids. Parenchyma cells are very conspicuous because of their organic inclusions, starch and lipids. Plasmodesmata in transverse and tangential walls of axial parenchyma cells and in end walls of ray parenchyma cells are regularly distributed and of uniform size, about 500 Å in diameter. In radial walls of axial parenchyma cells and horizontal walls of ray parenchyma cells plasmodesmata are located in primary pit-fields; there they are of variable size and often divided into several branches. The branches are confluent into a median nodule. Perforation of the transverse wall between two axial parenchyma cells and the resultant union of the cellular material of the two connected cells is reported.
    Type of Medium: Electronic Resource
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  • 10
    ISSN: 1432-2048
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary Sieve elements of various ages of Platycerium bifurcatum (Cav.) C. Chr. and Phlebodium aureum (L.) J. Sm. and older ones of Polypodium schraderi Mett. and Microgramma lycopodioides (L.) Copel. were examined with the electron microscope. Evidence was obtained which implicated the Golgi apparatus with the formation of refractive spherules in Platycerium and Phlebodium. In all four species the delimiting membranes of the refractive spherules eventually fuse with the plasmalemma in mature sieve elements, and the material comprising the spherules is liberated into the region of the wall.
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