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  • 1
    Electronic Resource
    Electronic Resource
    Zinc is associated with the β subunit of DNA-dependent RNA polymerase of Bacillus subtilis (1977)
    s.l. : American Chemical Society
    Biochemistry 16 (1977), S. 2880-2884 
    Details
    ISSN: 1520-4995
    Source: ACS Legacy Archives
    Topics: Biology , Chemistry and Pharmacology
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1021/bi00632a012
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  • 2
    Electronic Resource
    Electronic Resource
    Autogenous and post-transcriptional regulation of RNA polymerase synthesis (1980)
    Springer
    Molecular and cellular biochemistry 31 (1980), S. 177-196 
    Details
    ISSN: 1573-4919
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Chemistry and Pharmacology , Medicine
    Notes: Summary The regulation of gene expression was studied for the Escherichia coli rpoBC operon, which includes the genes, rpoB and rpoC, for the β and β′ subunits of RNA polymerase, and rplJ and rplL, for the two proteins, L10 and L7/12, of the 50S ribosome. The gene organization agrees well with the accumulated observations indicating the coordinate synthesis of RNA polymerase and ribosomes under various growth conditions for wild-type E. coli cells. On the other hand, the differential regulation of the two essential components observed under restrictive growth conditions, after addition of various drugs or with certain mutants, in particular those carrying mutations in the RNA polymerase genes, was found to take place through two novel regulation systems: The transcriptional termination at an internal attenuation site and the two autogenous and post-transcriptional controls, being specific for the two ribosomal protein genes and the two RNA polymerase subunit genes, respectively. The majority of the transcription initiated from the promoter rpoP β terminates at an attenuator site between the promoter-proximal rplJL and the promoter-distal rpoBC genes. The frequency of the attenuation seems to control the relative level of RNA polymerase synthesis to that of ribosomes. The expression of rpoBC genes is subject to an autogenous regulation, in which both RNA polymerase holoenzyme and α2β complex function as regulatory molecules with repressor activity. The autogenous regulation was found to operate at post-transcriptional step(s), probably at the level of translation. During the study on the regulation of RNA polymerase synthesis, we noticed that the rpoBC operon contained another autogenous regulation circuit, in which the synthesis of L10 and L7/12 was specifically repressed by the L10-L7/12 complex. Molecular mechanisms and physiological meanings of the novel regulations are discussed.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00225850
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  • 3
    Electronic Resource
    Electronic Resource
    Genetic mapping of the Escherichia coli gene for the stringent starvation protein and its dispensability for normal cell growth (1988)
    Springer
    Molecular genetics and genomics 211 (1988), S. 515-519 
    Details
    ISSN: 1617-4623
    Keywords: Stringent starvation protein ; Genetic mapping ; Dispensability
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary To determine its map position, the sSP gene was cloned into plasmid pBR322 and the recombinant plasmid was integrated into the chromosome of a polA mutant at the site of the sSP gene by homologous recombination. The chromosomal location of Ampr was then determined by P1 phage-mediated transduction. Thus, the sSP gene was mapped between gltB and glnF at min 69.5 on the Escherichia coli chromosome. Strains were constructed in which the sSP gene was brought under the control of the lac regulatory system. This indicated that the stringent starvation protein (SSP) is dispensable for growth, at least under normal culture conditions.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00425709
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  • 4
    Electronic Resource
    Electronic Resource
    Comparative studies of RNA polymerase subunits from various bacteria (1977)
    Springer
    Molecular genetics and genomics 154 (1977), S. 135-144 
    Details
    ISSN: 1617-4623
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary The molecular structure of RNA polymerases from Escherichia coli, Salmonella typhimurium, Salmonella anatum, Serratia marcescens, Aerobacter aerogenes, Proteus mirabilis and Bacillus subtilis were compared based on: i) inhibition of the enzyme activity by treatment with antibodies against E. coli RNA polymerase subunits; ii) analysis of antibody precipitates by sodium dodecyl sulfatepolyacrylamide gel electrophoresis; and iii) analysis of antibody precipitates by urea-isoelectrofocusing followed by sodium dodecyl sulfate-slab gel electrophoresis in the second dimension. All the bacterial RNA polymerases examined cross-react equally with anti-E. coli holopolymerase but exhibit different extents of cross-reaction with antibodies against individual subunits. Except for B. subtilis RNA polymerase, the molecular weight and isoelectric point of the enzyme subunits are close to those of E. coli polymerase. However, minor differences were found at least within the resolution of the techniques employed: S. anatum polymerase has σ subunit larger than E. coli σ subunit; P. mirabilis enzyme has σ subunit larger in size and more acidic in charge, and α subunit smaller and more basic than corresponding E. coli subunits. The electrophoretic map of B. subtilis enzyme subunits is completely different from that of E. coli enzyme.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00330829
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  • 5
    Electronic Resource
    Electronic Resource
    Cloning of the Escherichia coli gene for the stringent starvation protein (1985)
    Springer
    Molecular genetics and genomics 201 (1985), S. 151-157 
    Details
    ISSN: 1617-4623
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary In order to clone the Escherichia coli gene for the stringent starvation protein (SSP), we determined its N-terminal sequence as well as the sequence of two peptide fragments obtained by cyanogen bromide cleavage of the protein. We then chemically synthesized four sets of oligodeoxyribonucleotide mixtures that represented possible codon combinations for parts of these amino acid sequences. The synthetic oligonucleotides were labelled with 32P at their 5′-termini and used as hybridization probes to detect DNA fragments containing the complementary sequences. Genomic Southern hybridization of E. coli chromosomal DNA gave up to ten DNA fragments hybridizing with each probe but only a few hybridized with two or more of the probes. The latter fragments were coloned in pBR322. By determining partial base sequences with a rapid method and examining proteins encoded by the DNA fragments, we were able to show that we had isolated a clone containing the complete SSP structural gene.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00425652
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  • 6
    Electronic Resource
    Electronic Resource
    Biosynthesis of RNA polymerase in Escherichia coli (1978)
    Springer
    Molecular genetics and genomics 165 (1978), S. 7-14 
    Details
    ISSN: 1617-4623
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary Effect of temperature-sensitive, assembly-defective mutations in Escherichia coli RNA polymerase β (rpoB) or β′ subunit gene (rpoC) was investigated on the expression of wild-type rpoB +C+operon, which was introduced by infection of a lambda transducing phage λdrif + (rpoB +)-6 after UV-irradiation of the mutant cells. In rpoB2·rpoB7 strain which accumulates assembly-intermediates, free α, α2β complex and premature core, the expression of rpoB +C+operon measured by the rate of β subunit synthesis was considerably inhibited whereas that of EF(translation elongation factor)-Tu, ribosomal proteins L1 and L7/L12, and some λ-coded proteins remained unaffected. On the other hand, the expression was enhanced specifically for only rpoB +C+operon in either rpoC4 or rpoC1 mutants, which are defective in the association of α2β complex and β′ subunit or the activation of premature core enzyme, respectively. Upon preincubation of the mutant cells at 42° C prior to phage infection, during which assembly intermediates degraded rapidly, the rate of β subunit synthesis relative to other phage-corded proteins increased remarkably in rpoB2·rpoB7 mutant as well as in rpoC4 and rpoC1 mutants. These observations strongly suggested the autogenous regulation for at least ββ′ (rpoB +C+) operon by some trans-active diffusible protein complexes built of RNA polymerase subunits. Nature of the regulatory molecules is discussed.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00270370
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  • 7
    Electronic Resource
    Electronic Resource
    Autogenous regulation of the synthesis of ribosomal proteins, L10 and L7/12, in Escherichia coli (1980)
    Springer
    Molecular genetics and genomics 178 (1980), S. 483-486 
    Details
    ISSN: 1617-4623
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary The in vitro synthesis of Escherichia coli ribosomal proteins, L10 and L7/12, is specifically repressed by the addition of the L10-L7/12 complex, while that of other ribosomal proteins encoded by the neighboring operons is not affected. Thus the expression of the rpoBC operon is controlled by two autorepression systems, one for the two ribosomal proteins and the other for RNA polymerase β and β′ subunits, both operating probably at the translational level.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00270505
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  • 8
    Electronic Resource
    Electronic Resource
    Autogenous and post-transcriptional regulation of Escherichia coli RNA polymerase synthesis in vitro (1980)
    Springer
    Molecular genetics and genomics 179 (1980), S. 489-496 
    Details
    ISSN: 1617-4623
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary The in vitro synthesis of Escherichia coli RNA polymerase β and β′ subunits is repressed by either holoenzyme or α2β complex. The level of mRNA synthesized in this system, from the genes encoding ribosomal protein L7/12 (rplL) and RNA polymerase ββ′ subunits (rpoBC) was separately determined by DNA-RNA hybridization using the plasmids, pLOO and pLBC, each containing rplL and rplL plus rpoBC, respectively, as DNA probes. Although the synthesis of β and β′ polypeptides was repressed by exogenous addition of the autorepressors, the amount of rpoBC mRNA increased in parallel with the total RNA synthesis. Thus, the relative concentration of rpoBC mRNA stayed at a constant level. Based on the simultaneous determination of both protein and mRNA, it was concluded that the autogenous regulation of RNA polymerase β and β′ subunit synthesis operates post-transcriptionally, presumably at the translational level. Similarly, glycerol inhibition of β and β′ subunit synthesis was also found to be operative at post-transcriptional step(s). In contrast, the rifampicin induction of β and β′ subunit synthesis is due to both the increased transcription of rpoBC genes and the relaxation of the autogenous repression. The present study also implied that the majority of the transcription of the rpoBC operon terminates between rplL and rpoB genes. The difference in the intracellular content of ribosomal protein L7/12 and RNA polymerase ββ′ subunits might be attributed, at least in part, to transcriptional attenuation.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00271738
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  • 9
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    Autogenous and post-transcriptional regulation of RNA polymerase synthesis (1980)
    Springer
    Details
    Publication Date: 1980-01-01
    Print ISSN: 0300-8177
    Electronic ISSN: 1573-4919
    Topics: Biology , Chemistry and Pharmacology , Medicine
    Published by Springer
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  • 10
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    Effects of the copepod community structure on fecal pellet flux in Kagoshima bay, a deep, semi-enclosed embayment (2010)
    Springer
    Details
    Publication Date: 2010-09-18
    Print ISSN: 0916-8370
    Electronic ISSN: 1573-868X
    Topics: Geosciences , Physics
    Published by Springer
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