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  • 1
    Publication Date: 2016-03-01
    Print ISSN: 0306-2619
    Electronic ISSN: 1872-9118
    Topics: Energy, Environment Protection, Nuclear Power Engineering
    Published by Elsevier
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  • 2
    Publication Date: 2021-05-19
    Description: Fish culture has developoed in Yazd province of Iran since recent decade and the rainbow trout, Oncorhynchus mykiss is the most important fisheries product in the area. There is a few information about carcass composition of cultured fish in Iran. Rainbow trout samples were collected from 6 fish brackish and fresh water fish farms of Yazd province during February 2007. Fish muscle samples were freezed in -30 C after being homogenized, till laboratory analyses. Proximate composition of samples contained 17.05-18.53% crude protein, 2.35-5.13% total fat, 1.31-1.7% ash and 74.18-77.05% wet. Saturated fatty acids including palmitic acid (14.06-19.60%), stearic acid (6.44-6.1%), myristic acid (1.29-5.66%) and lauric acid (0.13-2.76), also unsaturated fatty acids oleic (30.24-40.14%) and linoleic (21.18-38.85%) were extracted from muscle tissue of fish of different farms, using GC. Other unsaturated fatty acids including -linolenic acid, EPA and DHA had low concentrations (1-3%) in samples. Vitamin E level was 4.33-94.34 g/100gr. Concentration of minerals in homogenated muscle tissues were as: Na 822.58-1052.78ppm, K 12272.75-13715.25ppm, Ca 104.75-171.03ppm, Fe 19-40ppm, Cu 0.044- 1.01ppb, Zn 7.02-12.47ppb, Cd 0.879-1.099ppb and Cr 1.895-3.86ppb. Heavy metal concentrations were lower than permitted values.
    Description: Iranian Fisheries Science Research Institute
    Description: Published
    Keywords: Oncorhynchus mykiss ; Rainbow trout ; Fish culture ; Tissues ; Brackish water ; Muscle
    Repository Name: AquaDocs
    Type: Report , Refereed
    Format: 80pp.
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  • 3
    Publication Date: 2021-05-19
    Description: Chitin nanofibers are prepared from the exoskeletons of shrimp by a simple mechanical treatment after a series of purification steps. The nanofibers have fine nanofibers networks with a uniform width of approximately 10 nm. Grinders and highpressure water jet systems are effective for disintegrating chitin into nanofibers. Acidic conditions are the key factor to facilitate mechanical fibrillation. Ultrafine fibers were successfully fabricated chitosan and fish skinextracted gelatin via electrospinning (ES). Important ES parameters, such as concentration of aqueous acid and fish gelatin solutions, and electric field intensity were examined to investigate the effects on the morphology of the gelatin nanofibers. Due to the poor mechanical properties of the fish gelatin membranes, composite nanofibers made of fish gelatin and poly(L-lactide)(PLLA) were produced with a novel solution. The introduction of PLLA remarkably improved the mechanical properties of the gelatin membranes. With a combination of good biocompatibility and mechanical properties, fish gelatin/PLGA blending non-woven mats are considered to be very promising in fish fillet coating application.. in this study, we fabricated a novel nanofibers composed of fish collagen (FC) and polycaprolactone (PCL) blends by using the electrospinning method. Nanofibers were characterized using a scanning electron microscope (SEM), and it was revealed that the diameter of nanofibers decreases as FC content was increased in the FC/PCL composite nanofibers. Several modifications to the chitin NF surface are achieved, including acetylation, deacetylation and maleylation. The results of this study revealed that: 1 –It is possible to produce Nanofibers from chitosan and fish gelatin. 2 – Covering and coating of processed fish by nanofibers are applicable and increasing the possibility of shell life for the processed fish. 3 – Nanofibers which have been produced from chitosan and fish gelation not only is environmentally friendly but also it will be eatable while has been covered for fish fillets. biocompatible chitosan and gelatin made from fish, fresh fish fillets do not have the ability to cover and packaging, but is edible and used.
    Description: Iranian Fisheries Science Research Institute
    Description: Published
    Keywords: Chitin ; Chitosan ; Nanofiber ; Chemical modification ; Fish ; Aquatic ; Maintenance
    Repository Name: AquaDocs
    Type: Report , Refereed
    Format: 66pp.
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  • 4
    Publication Date: 2021-05-19
    Description: Padina boergesenii is one of the most abundant brown algae distributed in the north of Persian Gulf and Oman Sea. In this study after sampling and preparation of Padina boergesenii by Chroform-Etanol (3-1) solvent and by Methanol has been extract. Separation and purification of the compounds was carried out using thin layer, general and inverse column chromatography, Cephadex and high-performance liquid chromatography (HPLC). Structural elucidation of the constituents was based on the data obtained from H-NMR, 13C-NMR, HSQC, HMBC, DEPT and Cephadex LH-20. The steroids compounds separated from above alga were identified as 22dehydrocholesterol (1), cholesterol (2), fucosterol (3), β-sitosterol (4), stigmasterol (5), ostreasterol (6) and two epimer of hyroxyestrol(7), based on their spectral data and from comparison with those previously reported in the literature.
    Description: Iranian Fisheries Science Research Institute
    Description: Published
    Keywords: Brown Algae ; Padina boergesenii ; Steroids compounds ; Extraction ; Purification ; Identification ; Amount verification ; Sargassum glaucescens ; Algae ; Abundant ; Sampling ; Chromatography ; Cephadex ; Dehydrocholesterol ; Cholesterol ; Fucosterol ; β-sitosterol ; Stigmasterol ; Ostreasterol
    Repository Name: AquaDocs
    Type: Report , Refereed
    Format: 42pp.
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  • 5
    Publication Date: 2021-05-19
    Description: Sponges are the simplest multicellular animals that lack defense mechanisms and rely on chemical defense that have been used by mankind to develop antimicrobial drugs against diseases. The present study was designed to demonstrate the antibacterial and antifungal activities of marine sponge Dysidea pallescens semipolar and nonpolar extracts. In this study, D. pallescens were collected from Hengam Island in the Persian Gulf. The extracts were produced by Bligh and Dyer method. Broth Dilution Methods were used to check the antimicrobial activity of D. pallescens extracts against Escherichia coli (ATCC 15224), Pseudomonas aeruginosa (ATCC 25619), Staphylococcus aureus aureus (ATCC 1764), Bacillus subtilis pizizenii (ATCC 6633), Candida albicans (ATCC10231) and Aspergillus fumigates (PTCC5009). The results showed diethyl ether extract has bactericidal activity against S. aureus aureus (MBC=10mg/mL) and B. subtilis spizizenii (MBC=20mg/mL). D. pallescens diethyl ether extract showed a very weak antifungal activity but methanol extract showed fungicidal activity against A. fumigates (MFC=5mg/mL) and C. albicans (MFC=1.5 mg/mL). Therefore nonpolar-semipolar secondary metabolites of D. pallescens solutions in diethyl ether have shown significant antibacterial activity and polar- secondary metabolites solutions in methanol have shown significant antifungal activity.
    Description: Published
    Keywords: Biotechnology ; Dysidea pallescens ; Pseudomonas aeruginosa ; Escherichia coli ; Bacillus subtilis pizizenii ; Staphylococcus aureus aureus ; Candida albicans ; Aspergillus fumigates ; Antibacterial ; Antifungal ; Marine sponge
    Repository Name: AquaDocs
    Type: Journal Contribution , Refereed
    Format: pp.200-209
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  • 6
    Publication Date: 2021-05-19
    Description: Sea cucumbers produce various bioactive compounds especially secondary metabolites including Saponin. The aim of this study was to investigate antibacterial activity of the Saponin extracted from the Persian Gulf sea cucumber (Stichopus hermanni) in some gram positive and negative bacteria. Extraction was first done by ethanol 70% and then Saponins were separated through column chromatography with silica gel. Extracted Saponins were identified through thin layer chromatography (TLC) and high performance thin layer chromatography (HPTLC). Minimum inhibitory concentration (MIC) and minimum bactericidal concentration (MBC) were investigated via tubular dilution. Two groups of steroidal and glycosides Saponins with Rf 0.9 and 0.05 were respectively identified in fractions extracted by TLC. HPTLC results for a sample of extracted Saponin confirmed the existence of Saponin in sea cucumber regarding the Rf. The gram negative bacterium Pseudomonas aeroginosa was the most resistant one to steroidal and glycosides- steroidal Saponins with MIC of 500 and 400 micg/ml, respectively. Steroidal and glycosides- steroidal Saponins showed no bactericidal effect in gram negative bacterium Pseudomonas aeroginosa. Among the gram positive bacteria, the highest MBC of 500 micg/ml belonged to glycosides- steroidal Saponin for Staphylococcus aureus. The higher MIC in gram negative bacteria approves the lower antibacterial property of the steroidal and glycosides Saponin extracted from sea cucumber Stichopus hermanni in these bacteria compared to the gram positive ones.
    Description: Published
    Keywords: Sea cucumber Stichopus hermanni ; Steroidal Saponin ; Minimum inhibitory concentration ; Gram- negative bacteria ; Antibacterial ; Stichopus hermanni ; Pseudomonas aeroginosa ; Staphylococcus aureus
    Repository Name: AquaDocs
    Type: Journal Contribution , Refereed
    Format: pp.59-69
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  • 7
    Publication Date: 2021-05-19
    Description: Chemical-ecological studies of sponges show that the secondary metabolites are only defense system. Nowadays natural products with biological activities are used as drugs for treatment of human diseases. One of the most important biological activities of sponges is cytotoxic activity. This study investigated the cytotoxic activities of α-Santonin compound which extracted from Dysidea avara. In this study, dried powder of sea sponge was extracted by acetone solvent. Then, in order to isolate the α-Santonin compound, the extract was washed by silica gel column chromatography with N-hexane and ethyl acetate solvents. The IC50 value of this compound was measured by XTT assay on cancer cell lines, Jurkat/E6-1, KB/C152 and healthy Hek293 cells. The α-Santonin compound with the chemical formula C15H18O3, which belongs to the Sesquiterpene-lactone group, was identified by gas chromatography, with a purity of 91% in fraction No. 24. The IC50 value of the α-Santonin extracted from sea sponge compared to the oral epithelial cancer cell line and lymphocyte cell line was 88.90 μg/ml and 38.09 μg/ml respectively. Our study demonstrated that α-Santonin extracted from D. avara has an extremely potent cytotoxic effect that could be used as an anticancer agent.
    Description: Published
    Keywords: Sponge ; LC50 ; Santonin ; Hengam Island
    Repository Name: AquaDocs
    Type: Journal Contribution , Refereed
    Format: pp.39-47
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  • 8
    Publication Date: 2021-05-19
    Description: Specialists in Marine biotechnology using marine biological engineering and scientific principles to develop natural products and regarded services. One of the most important applications of this new branch is produce the natural products such as enzymes, natural colors, vaccines and drugs and etc. which produced from marine living organisms. These products are used in various industries such as pharmaceutical, food or health industry. In the current study, we try to remark the application of marine biological products in the northern (Caspian Sea), southern (Persian Gulf and Oman Sea) or internal waters of Iran into six categories, including aquaculture, pharmaceutical industry, food, healthcare and cosmetic industries. For this purpose, the required information will be collected using investigations which conducted inside and also outside of our country by reviewing and analysis of derived data by attention to our economical facilities for national self-sufficiency in production. Applied research projects will be introduced in three parts including research, technology development and pilot plant as well. Hope that using this strategic document will be effective and practical to production of biological products from marine resources until 2025.
    Description: Iranian Fisheries Science Research Institute
    Description: Published
    Keywords: Enzymes ; Marine ; Biological ; Biotechnology ; Aquatic
    Repository Name: AquaDocs
    Type: Report , Refereed
    Format: 131pp.
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  • 9
    Publication Date: 2021-05-19
    Description: Sponges are the most primitive multicellular eukaryotic organisms and sessile without defense organs therefore, they produce different secondary metabolites to protect themselves from predators and pathogens in their environment. This study aims to evaluation of cytotoxic properties of hexane and dichloromethane parts of methanol extract of the sponge (Ircinia mutans), collected from Larak Island. Hexane and dichloromethane parts were extracted by liquid-liquid extraction from methanol extract then tested by MTT colorimetric assay on three cancer cell lines MOLT-4, MCF-7 and HT-29. The hexane part with IC50 value 11.53 ± 1.3, 41.64 ± 3.5 and 14.57 ± 1.5 μg/mL, and the dichloromethane part with IC50 value 12.51 ± 0.8, 26.6 ± 2.2 and 7.47 ± 0.97 μg/mL respectively, on three mentioned cell line showed moderate to strong cytotoxic activity on all three cancer cell lines. The results also indicate that the dichloromethane part has stronger cytotoxic activity than the hexane part. This study's findings showed the potential of Persian Gulf sponges as valuable sources of new anti-cancer compounds.
    Description: Published
    Keywords: Sponge ; Secondary metabolite ; Cytotoxic activity
    Repository Name: AquaDocs
    Type: Journal Contribution , Refereed
    Format: pp.105-114
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  • 10
    Publication Date: 2021-05-19
    Description: Marine environment is the main source of natural bioactive products whose chemical-structural properties are not similar with other natural products of plants and other inland organisms. The present study was carried out to evaluate antibiotic properties of diethyl ether and methanol extracts of two marine species including starfish and carpet anemone. The samples of starfish and carpet anemone were collected from the Lark Island coasts. Extraction was done using diethyl ether and methanol solvents and antibiotic properties analysis on Escherichia coli and Bacillus subtilis were evaluated using agar diffusion method. Then tube dilution to determine the minimum inhibitory concentration (MIC) and minimum bactericidal concentration (MBC). According to the results, diethyl ether extract from carpet anemone had no inhibitory effect on E. coli bacteria. While, starfish methanol extract (40 mg/ml) showed a growth inhibitory effect on E. coli bacteria. The starfish methanol extract (30 and 50 mg/ml) had a growth inhibitory effect on B. subtilis bacteria. Carpet anemone methanol extract (40 mg/ml) cause a growth inhibitory effect on E. coli, but did not have a deleterious effect. Starfish methanol extract showed the highest growth inhibitory effect on B. subtilis and created a growth inhibitory circle zoon (12.1±0.8 mm diameter). The results showed that the methanolic extract of the starfish has higher growth inhibitory effect on the two studied bacterial species. In conclusion, methanolic extracts of the starfish were effective in inhibiting the growth of Bacillus subtilis bacteria and methanolic extract of carpet anemone in inhibiting E. coli bacteria growth.
    Description: Published
    Keywords: Sarcophyton spp. ; Antibacterial ; Agar ; Bioactive ; Extraction ; Growth
    Repository Name: AquaDocs
    Type: Journal Contribution , Refereed
    Format: pp.195-205
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