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  • 11
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    Development of a Sensitive Bioassay for the Analysis of IGF-Related Activation of AKT/mTOR Signaling in Biological Matrices (2021)
    Molecular Diversity Preservation International
    Details
    Publication Date: 2021-02-24
    Description: The bioactivity of the IGF system is not a function of isolated hormone concentrations in a given biological matrix. Instead, the biological activities of IGFs are regulated by IGFBPs, IGFBP proteases, and inhibitors of IGFBP proteases. Therefore, assays based on IGF-related bioactivity may describe functions of the complete IGF system in a given biological matrix. Of particular interest are the IGF system effects on the AKT/mTOR pathway, as a dominant system for controlling growth, metabolism, and aging. In order to improve the sensitivity of IGF-dependent bioactivity, we made use of the known short-term and enhancing effects of IGFBP2 on the intracellular PI3K pathway. As a specific readout of this pathway, and further as a marker of the mTOR pathway, we assessed the phosphorylation of AKT-Ser473. Preincubation using IGFBP2 enhanced IGF1-dependent AKT-Ser473 phosphorylation in our experimental system. The assay’s specificity was demonstrated by inhibition of IGF1 receptors outside or inside the cell, using antiserum or small molecule inhibitors, which reduced AKT phosphorylation in response to exogenous IGF1 (p 〈 0.05). The maximal response of AKT phosphorylation was recorded 15 to 60 min after the addition of IGF1 to cell monolayers (p 〈 0.001). In our cellular system, insulin induced AKT phosphorylation only at supra-physiological concentrations (µM). Using this novel assay, we identified the differential biological activity of the IGF system in AKT-Ser473 phosphorylation in serum (mouse, naked mole rat, and human), in cerebrospinal fluid (human), and in colostrum or mature milk samples (dairy cow). We have developed a sensitive and robust bioassay to assess the IGF-related activation of the AKT/mTOR pathway. The assay works efficiently and does not require expensive cell culture systems. By using capillary immuno-electrophoresis, the readout of IGF-related bioactivity is substantially accelerated, requiring a minimum of hands-on time. Importantly, the assay system is useful for studying IGF-related activity in the AKT/mTOR pathway in a broad range of biological matrices.
    Electronic ISSN: 2073-4409
    Topics: Biology
    Published by Molecular Diversity Preservation International
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    PAPER CURRENT
  • 12
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    Modellbasierte prädiktive Längsdynamikregelung für künftige Fahrerassistenz- und Automatisierungssysteme (2024)
    KIT Scientific Publishing
    Details
    Publication Date: 2024-04-11
    Description: A fundamental requirement for future automated vehicles is that curbs and other bumps in the road can be driven over safely, reliably and accurately. This work presents a concept for a model-based, predictive longitudinal vehicle dynamics control. It demonstrates in practice how the knowledge of the road profile ahead can be used to both increase the control performance and improve the driving comfort.
    Keywords: Preview; Longitudinal Motion Control; Road Profile Disturbances; Vehicle Dynamics; Vorausschau; Längsregelung; Störungen durch Fahrbahnprofil; Two Point Tire Model; Fahrzeugdynamik ; thema EDItEUR::T Technology, Engineering, Agriculture, Industrial processes::TH Energy technology and engineering::THR Electrical engineering ; thema EDItEUR::T Technology, Engineering, Agriculture, Industrial processes::TH Energy technology and engineering::THR Electrical engineering
    Language: German
    Format: image/jpeg
    Format: image/jpeg
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    E-BOOK
  • 13
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    Incubation results from ice-rich permafrost deposits in Northeast Siberia (2018)
    PANGAEA
    In:  Supplement to: Walz, Josefine; Knoblauch, Christian; Tigges, Ronja; Opel, Thomas; Schirrmeister, Lutz; Pfeiffer, Eva-Maria (2018): Greenhouse gas production in degrading ice-rich permafrost deposits in northeastern Siberia. Biogeosciences, 15(17), 5423-5436, https://doi.org/10.5194/bg-15-5423-2018
    Details
    Publication Date: 2023-03-16
    Description: CO2 and CH4 production in aerobic and anaerobic incubation in sediments samples from Muostakh Island (sample code MUO12), the Buor Khaya Peninsula (BK8), and Bol'shoy Lyakhovsky (L14-05 and L14-02). CO2 and CH4 production at 4°C over a 134-day (MUO12) or a 785-day incubation period (BK8, L14-05, L14-02) measured by gas chromatography.
    Keywords: AWI_Envi; AWI_Perma; Permafrost Research; Polar Terrestrial Environmental Systems @ AWI
    Type: Dataset
    Format: application/zip, 6 datasets
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    DATA PANGAEA
  • 14
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    Lateral and vertical carbon fluxes from the polygonal tundra in the Lena River Delta, Siberia (summer 2014) (2022)
    PANGAEA
    Details
    Publication Date: 2023-11-16
    Description: This dataset contains observations of water discharge rates and concentrations of dissolved organic carbon (DOC) and dissolved inorganic carbon (DIC) from a polygonal tundra site in the Lena River Delta, Russia. This dataset also contains lateral carbon fluxes of DOC and DIC that were estimated from these observations. Additionally, this dataset contains vertical fluxes of carbon dioxide and methane from the same study site. All observations were recorded on Samoylov Island (N 72.377188, E 126.495144) in the summer of 2014. The abbreviations A1, A2 and B refer to three outflows on the island where the hydrological parameters were observed (A1: N 72.379991, E 126.480886; A2: N 72.380134, E 126.481433; B: N 72.381348, E 126.483482). All outflows were approximately 10 meters. More information can be found in https://doi.org/10.5194/bg-19-3863-2022.
    Keywords: AWI Arctic Land Expedition; Carbon, inorganic, dissolved; Carbon, inorganic, dissolved, flux; Carbon, inorganic, dissolved, standard deviation; Carbon, organic, dissolved; Carbon, organic, dissolved, flux; Carbon, organic, dissolved, standard deviation; Carbon dioxide, flux; Carbon dioxide, flux, standard deviation; Carbon in Permafrost / Kohlenstoff im Permafrost; CARBOPERM; CH4 flux; CLICCS; Cluster of Excellence: Climate, Climatic Change, and Society; CO2 flux; DATE/TIME; Date/Time local; DIC; DIC flux; Discharge; DOC; DOC flux; Formation, turnover and release of carbon in Siberian permafrost landscapes; KoPF; Lena River Delta; Methane, flux; Methane, flux, standard deviation; Mini-Diver and RBC flume; Mini-Diver and V-notch weir; MULT; Multiple investigations; Multi-Sensor-Module MSM-S2, UIT Germany; polygon tundra; Quality flag, carbon dioxide, flux; Quality flag, methane, flux; Quality flag according to Mauder and Foken, 2004; RU-Land_2014_Lena; RU-Land_2014_Lena-Discharge; Samoylov Island, Lena Delta, Siberia; TOC analyzer (Shimadzu)
    Type: Dataset
    Format: text/tab-separated-values, 254196 data points
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    DATA PANGAEA
  • 15
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    Characteristics of permafrost-affected soils of the northeast Siberian polygonal tundra (2016)
    PANGAEA
    In:  University of Hamburg, Germany
    Details
    Publication Date: 2024-01-18
    Keywords: Arctic Tundra; Carbon, organic, per unit sediment mass; carbon isotope; DEPTH, soil; Depth, soil, maximum; Depth, soil, minimum; Event label; EXP; Experiment; Latitude of event; Layer description; Longitude of event; nitrogen; Nitrogen, total; organic carbon; pH, soil; Samoylov_Polygon1; Samoylov_Polygon2; Samoylov_Polygon3; Samoylov Island, Lena Delta, Siberia; soil depth; Soil Horizons/Profile; soil pH; δ13C, organic carbon
    Type: Dataset
    Format: text/tab-separated-values, 147 data points
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    DATA PANGAEA
  • 16
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    Soil and incubation data of permafrost-affected soils of the northeast Siberian polygonal tundra (2016)
    PANGAEA
    In:  University of Hamburg, Germany | Supplement to: Walz, Josefine; Knoblauch, Christian; Böhme, Luise; Pfeiffer, Eva-Maria (2017): Regulation of soil organic matter decomposition in permafrost-affected Siberian tundra soils - Impact of oxygen availability, freezing and thawing, temperature, and labile organic matter. Soil Biology and Biochemistry, 110, 34-43, https://doi.org/10.1016/j.soilbio.2017.03.001
    Details
    Publication Date: 2024-01-18
    Description: The large amounts of soil organic matter (SOM) in permafrost-affected soils are prone to increased microbial decomposition in a warming climate. The environmental parameters regulating the production of carbon dioxide (CO2) and methane (CH4), however, are insufficiently understood to confidently predict the feedback of thawing permafrost to global warming. Therefore, the effects of oxygen availability, freezing and thawing, temperature, and labile organic matter (OM) additions on greenhouse gas production were studied in northeast Siberian polygonal tundra soils, including the seasonally thawed active layer and upper perennially frozen permafrost. Soils were incubated at constant temperatures of 1 °C, 4 °C, or 8 °C for up to 150 days. CO2 production in surface layers was three times higher than in the deeper soil. Under anaerobic conditions, SOM decomposition was 2-6 times lower than under aerobic conditions and more CO2 than CH4 was produced. CH4 contributed less than 2% to anaerobic decomposition in thawed permafrost but more than 20% in the active layer. A freeze-thaw cycle caused a short-lived pulse of CO2 production directly after re-thawing. Q10 values, calculated via the equal-carbon method, increased with soil depth from 3.4 ± 1.6 in surface layers to 6.1 ± 2.8 in the permafrost. The addition of plant-derived labile OM (13C-labelled Carex aquatilis leaves) resulted in an increase in SOM decomposition only in permafrost (positive priming). The current results indicate that the decomposition of permafrost SOM will be more strongly influenced by rising temperatures and the availability of labile OM than active layer material. The obtained data can be used to inform process-based models to improve simulations of greenhouse gas production potentials from thawing permafrost landscapes.
    Keywords: active layer; carbon dioxide; carbon isotope; Methane; nitrogen; organic carbon; permafrost; Soil Horizons/Profile
    Type: Dataset
    Format: application/zip, 7 datasets
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    DATA PANGAEA
  • 17
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    Aerobic incubation results from Bol'shoy Lyakhovsky Island (L14) (2018)
    PANGAEA
    Details
    Publication Date: 2024-04-25
    Keywords: AWI_Envi; AWI_Perma; AWI Arctic Land Expedition; Bolshoy-Lyakhovsky_Island_2014; Bolshoy Lyakhovsky Island, East Siberia, Russia; Carbon dioxide, production, aerobic, CO2-C per mass soil organic carbon; Day of experiment; DEPTH, soil; Event label; L14-02; L14-05; LAND; Permafrost Research; Polar Terrestrial Environmental Systems @ AWI; RU-Land_2014_BolshoyLyakhovsky; Sample ID; Sampling/measurement on land
    Type: Dataset
    Format: text/tab-separated-values, 550 data points
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    DATA PANGAEA
  • 18
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    Anaerobic carbon dioxide and methane production data of control and freeze-thaw permafrost-affected soils of the northeast Siberian polygonal tundra (2016)
    PANGAEA
    In:  University of Hamburg, Germany
    Details
    Publication Date: 2024-04-25
    Keywords: active layer; carbon dioxide; Carbon dioxide, production, anaerobic, CO2-C per mass soil organic carbon; Carbon dioxide, production, anaerobic, standard deviation; Carbon dioxide, production rate, anaerobic, CO2-C per mass soil organic carbon; Comment; Day of experiment; DEPTH, soil; Depth, soil, maximum; Depth, soil, minimum; EXP; Experiment; Methane; Methane, production, anaerobic, CH4-C per mass soil organic carbon; Methane, production, anaerobic, standard deviation; Methane, production rate, anaerobic, CH4-C per mass soil organic carbon; permafrost; Samoylov_Polygon2; Samoylov Island, Lena Delta, Siberia
    Type: Dataset
    Format: text/tab-separated-values, 1590 data points
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    DATA PANGAEA
  • 19
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    Aerobic carbon dioxide production data of permafrost-affected soils of the northeast Siberian polygonal tundra (2016)
    PANGAEA
    In:  University of Hamburg, Germany
    Details
    Publication Date: 2024-04-25
    Keywords: active layer; carbon dioxide; Carbon dioxide, production, aerobic, CO2-C per mass soil organic carbon; Carbon dioxide, production, aerobic, standard deviation; Day of experiment; Event label; EXP; Experiment; Latitude of event; Longitude of event; permafrost; Samoylov_Polygon1; Samoylov_Polygon2; Samoylov Island, Lena Delta, Siberia
    Type: Dataset
    Format: text/tab-separated-values, 930 data points
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    DATA PANGAEA
  • 20
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    Aerobic carbon dioxide production data of permafrost-affected soils of the northeast Siberian polygonal tundra under different temperature treatments (2016)
    PANGAEA
    In:  University of Hamburg, Germany
    Details
    Publication Date: 2024-04-25
    Keywords: active layer; carbon dioxide; Carbon dioxide, production, aerobic, CO2-C per mass soil organic carbon; Carbon dioxide, production, aerobic, standard deviation; Comment; Day of experiment; DEPTH, soil; Depth, soil, maximum; Depth, soil, minimum; EXP; Experiment; permafrost; Samoylov_Polygon3; Samoylov Island, Lena Delta, Siberia
    Type: Dataset
    Format: text/tab-separated-values, 818 data points
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    DATA PANGAEA
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