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  • 11
    Electronic Resource
    Electronic Resource
    Springer
    Cellular and molecular life sciences 39 (1983), S. 176-177 
    ISSN: 1420-9071
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary Following treatment with sodium selenite, pentobarbital hypnosis was prolonged in male rats. The maximal effect occurred at 3–4 days following selenium treatment and the threshold dose for the effect was found to be 2.4 mg Se/kg (i.p.).
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  • 12
    Electronic Resource
    Electronic Resource
    Springer
    Cellular and molecular life sciences 30 (1974), S. 528-529 
    ISSN: 1420-9071
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Zusammenfassung Nachweis, dass Cadmium bei Ratten die Hexobarbital-Schlafdauer erhöht. Eine wirksame Reaktion war bereits bei einer Cadmium-Dosis von 840 μg/kg beobachtet, und die Schlafdauer wurde bereits nach einer einzigen Dosis vom 1. bis zum 10. Tage erhöht, wobei zwischen dem 2. und 5. Tage die Maximalwirkung beobachtet wurde.
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  • 13
    ISSN: 1432-2242
    Keywords: Key words Heterozygosity ; Molecular markers ; Genetic map ; Quantitative trait loci ; Theobroma cacao
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract A genetic linkage map of Theobroma cacao (cocoa) has been constructed from 131 backcross trees derived from a cross between a single tree of the variety Catongo and an F1 tree from the cross of Catongo by Pound 12. The map comprises 138 markers: 104 RAPD loci, 32 RFLP loci and two morphologic loci. Ten linkage groups were found which cover 1068 centimorgans (cM). Only six (4%) molecular-marker loci show a significant deviation from the expected 1:1 segregation ratio.The average distance between two adjacent markers is 8.3 cM. The final genome-size estimates based on two-point linkage data ranged from 1078 to 1112 cM for the cocoa genome. This backcross progeny segregates for two apparently single gene loci controlling (1) anthocyanidin synthesis (Anth) in seeds, leaves and flowers and (2) self-compatibility (Autoc). The Anth locus was found to be 25 cM from Autoc and two molecular markers co-segregate with Anth. The genetic linkage map was used to localize QTLs for early flowering, trunk diameter, jorquette height and ovule number in the BC1 generation using both single-point ANOVA and interval mapping. A minimum number of 2–4 QTLs (P〈0.01) involved in the genetic expression of the traits studied was detected. Coincident map locations of a QTL for jorquette height and trunk diameter suggests the possibility of pleiotropic effects in cocoa for these traits. The combined estimated effects of the different mapped QTLs explained between 11.2% and 25.8% of the phenotypic variance observed in the BC1 population.
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  • 14
    Electronic Resource
    Electronic Resource
    Springer
    Theoretical and applied genetics 90 (1995), S. 269-274 
    ISSN: 1432-2242
    Keywords: Random amplified polymorphic DNA Mango ; Genetic diversity ; Cultivar identification
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Twenty-five accessions of mango were examined for random amplified polymorphic DNA (RAPD) genetic markers with 80 10-mer random primers. Of the 80 primers screened, 33 did not amplify, 19 were monomorphic, and 28 gave reproducible, polymorphic DNA amplification patterns. Eleven primers were selected from the 28 for the study. The number of bands generated was primer- and genotype-dependent, and ranged from 1 to 10. No primer gave unique banding patterns for each of the 25 accessions; however, ten different combinations of 2 primer banding patterns produced unique fingerprints for each accession. A maternal half-sib (MHS) family was included among the 25 accessions to see if genetic relationships could be detected. RAPD data were used to generate simple matching coefficients, which were analyzed phenetically and by means of principal coordinate analysis (PCA). The MHS clustered together in both the phenetic and the PCA while the randomly selected accessions were scattered with no apparent pattern. The uses of RAPD analysis for Mangifera germ plasm classification and clonal identification are discussed.
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  • 15
    ISSN: 1432-2242
    Keywords: Heterozygosity ; Molecular markers ; Genetic map ; Quantitative trait loci ; Theobroma cacao
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract A genetic linkage map of Theobroma cacao (cocoa) has been constructed from 131 backcross trees derived from a cross between a single tree of the variety Catongo and an F1 tree from the cross of Catongo by Pound 12. The map comprises 138 markers: 104 RAPD loci, 32 RFLP loci and two morphologic loci. Ten linkage groups were found which cover 1068 centimorgans (cM). Only six (4%) molecular-marker loci show a significant deviation from the expected 1∶1 segregation ratio.The average distance between two adjacent markers is 8.3 cM. The final genome-size estimates based on two-point linkage data ranged from 1078 to 1112 cM for the cocoa genome. This backcross progeny segregates for two apparently single gene loci controlling (1) anthocyanidin synthesis (Anth) in seeds, leaves and flowers and (2) self-compatibility (Autoc). The Anth locus was found to be 25 cM from Autoc and two molecular markers co-segregate with Anth. The genetic linkage map was used to localize QTLs for early flowering, trunk diameter, jorquette height and ovule number in the BC1 generation using both single-point ANOVA and interval mapping. A minimum number of 2–4 QTLs (P〈0.01) involved in the genetic expression of the traits studied was detected. Coincident map locations of a QTL for jorquette height and trunk diameter suggests the possibility of pleiotropic effects in cocoa for these traits. The combined estimated effects of the different mapped QTLs explained between 11.2% and 25.8% of the phenotypic variance observed in the BC1 population.
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  • 16
    Electronic Resource
    Electronic Resource
    Springer
    Plant ecology 2 (1950), S. 331-340 
    ISSN: 1573-5052
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
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  • 17
    Electronic Resource
    Electronic Resource
    Springer
    Plant ecology 3 (1952), S. 350-406 
    ISSN: 1573-5052
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Conclusions Malgré ses enclaves de savance et la prairie, riches en espèces tropicales xérophiles, le territoire étudié appartient incontestablement au domaine forestier guineo-équatorial. Plusieurs climax, tous forestiers, appartenant, suivant les conditions locales, aux ordres des Triplochitetalia, Lophiretalia et Parinarietalia, y sont représentés. En fait, cette végétation primitive est profondément dégradée, aux basses altitudes par les défrichements, et sur les crêtes par les feux. C'est dire tout l'intérêt que présente, dans ce territoire, la protection de la Nature; la mise en réserve naturelle intégrale du massif du Nimba, promulguée en juillet 1944 sur l'initiative de M. le Professeur Heim et de M. le Professeur Monod, Directeur de l'Institut Français d'Afrique Noire, préservera de la destruction les richesses botaniques de cette région montagneuse.
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  • 18
    Electronic Resource
    Electronic Resource
    Springer
    Molecular genetics and genomics 105 (1969), S. 84-86 
    ISSN: 1617-4623
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary In a strain of the mutantw (yellow eye colour, sex-linked and sex-limited to the females) ofCalliphora erythrocephala yellow-eyed males have been found. In connexion with the mechanism of sex-determination in this fly, their origin is explained by an alteration on the Y-chromosome (one of the IIIrd large chromosomes) in form of either a pre-meiotic cross-over or a spontaneous mutation on thew +-locus. For being recessive, it can become manifest only in thew strain.
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  • 19
    Electronic Resource
    Electronic Resource
    Springer
    Molecular genetics and genomics 260 (1999), S. 569-573 
    ISSN: 1617-4623
    Keywords: Key wordsPCR cloning ; Sticky ends ; Abasic primer
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract A novel method for the directional cloning of native PCR products was developed. Abasic sites in DNA templates make DNA polymerases stall at the site during synthesis of the complementary strand. Since the 5′ ends of PCR product strands contain built-in amplification primers, abasic sites within the primers result in the formation of 5′ single-stranded overhangs at the ends of the PCR product, enabling its direct ligation to a suitably cleaved cloning vector without any further modification. This “autosticky PCR” (AS-PCR) overcomes the problems caused by end sensitivity of restriction enzymes, or internal restriction sites within the amplified sequences, and enables the generation of essentially any desired 5′ overhang.
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  • 20
    ISSN: 1573-0662
    Keywords: Arctic pollution ; aerosols ; condensation nuclei ; extinction coefficient ; AGASP ; polar meteorology ; Alert ; N.W.T.
    Source: Springer Online Journal Archives 1860-2000
    Topics: Chemistry and Pharmacology , Geosciences
    Notes: Abstract In April 1986, a well-instrumented NOAA WP-3D research aircraft conducted three flights in the Canadian Arctic tied to the Canadian Atmospheric Environment Service baseline station in Alert, Northwest Territories. Two of the flights were coordinated with the National Aeronautical Establishment of Canada Twin Otter and the University of Washington C-131 research aircraft. The haze observed in the Canadian Arctic was well-aged and mixed throughout the troposphere in concentrations well below those observed during the previous weeks in the Alaskan Arctic. Over the ice, beneath the surface temperature inversion, ozone was generally depleted to near zero. Over the coast at Alert, there is evidence that topography and downslope winds reduce the strength of the inversion, thus allowing lower tropospheric gases and aerosols to mix down to the surface. At the top of the troposphere, an aerosol-depleted region was observed. In the lower stratosphere, aerosol concentrations were elevated above those observed in the troposphere.
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