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  • 11
    Electronic Resource
    Electronic Resource
    Microbial production of propionic acid and vitamin B12 using molasses or sugar (1994)
    Springer
    Applied microbiology and biotechnology 41 (1994), S. 378-383 
    Details
    ISSN: 1432-0614
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Process Engineering, Biotechnology, Nutrition Technology
    Notes: Abstract With a cell concentration of 125 g dry biomass l–1 and a dilution rate of 0.1 h–1, Propionibacterium acidipropionici produces 30 g propionic acid l–1 from sugar with a productivity of 3 g l–1 h–1. The yield of propionic acid is approx. 0.36–0.45 g propionic acid g–1 sucrose and is independent of the dilution rate and cell concentration. Acetic acid is an unwanted by-product in the production of propionic acid. The concentration of acetic acid only increases slightly when the cell concentration is increased. A two-stage fermentation process was developed for the conversion of sugar or molasses of various types to propionic acid and vitamin B12. By fermentation of blackstrap molasses (from sugar beet and sugar cane) in the first fermentation stage 17.7 g propionic acid l–1 with a yield of 0.5 g propionic acid g–1 carbohydrate was produced with a dilution rate of 0.25 h–1. In the second stage 49 mg vitamin B12 l–1 was produced at a dilution rate of 0.03 h–1.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF01982523
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  • 12
    Electronic Resource
    Electronic Resource
    Effect of the oxygen supply on pattern of growth and corrinoid and organic acid production of Propionibacterium shermanii (1998)
    Springer
    Applied microbiology and biotechnology 49 (1998), S. 732-736 
    Details
    ISSN: 1432-0614
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Process Engineering, Biotechnology, Nutrition Technology
    Notes: Abstract Propionibacterium shermanii CDB 10014 is able to grow even at high oxygen transfer rates (24.0 mmol O2 l−1 h−1), in contrast to reports in the specialised literature, where all Propionibacteria are considered oxygen-sensitive microorganisms. Propionic acid is the main product in anaerobiosis. The presence of oxygen in the system leads to an inhibition of propionic acid production while acetic acid formation is enhanced. At high oxygen supply rates no propionic acid is produced and acetic acid is the main product. Lactic acid is also produced in reasonable quantities (2.7 g l−1). The growth rate (μmax) is higher in anaerobiosis (0.19 h−1) than in aerobiosis (0.12–0.15 h−1). The cell yield is higher in aerobiosis (0.18–0.22 g g−1) than in anaerobiosis (0.14 g g−1) suggesting the oxidative metabolism of glucose by Propionibacterium shermanii CDB 10014. No corrinoid production was detected at oxygen transfer rates of more than 13.6 mmol l−1 h−1.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/s002530051239
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  • 13
    Electronic Resource
    Electronic Resource
    Effect of oxygen supply on biomass, organic acids and vitamin B12 production by Propionibacterium shermanii (1998)
    Springer
    World journal of microbiology and biotechnology 14 (1998), S. 843-846 
    Details
    ISSN: 1573-0972
    Keywords: Oxygen ; Propionibacterium ; propionic acid ; vitamin B12.
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Process Engineering, Biotechnology, Nutrition Technology
    Notes: Abstract Propionibacterium shermanii CDB 10015 was able to grow at different volumetric oxygen transfer coefficients (KLa) of 10, 22, 53h−1. These results demonstrate that this bacterium, known as anaerobic, is able to grow well under aerobic conditions. The cell biomass increased from 7.9 in anaerobic conditions to 18.3g/l at KLa 53h−1, increasing also the cell yield from 0.3 to 0.7g/g. The organic acid production pattern also changed with aeration. The acetic: propionic acid ratio increased from 0.38 in anaerobiosis to 6.25 at KLa 53h−1. The vitamin B12 production decreased from 3.1mg/l in anaerobiosis to 0.5mg/l at KLa 53h−1.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1023/A:1008868907251
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  • 14
    Electronic Resource
    Electronic Resource
    Short communication: In vitro evaluation of a Bacillus sp. for the biological control of the coffee phytopathogen Mycena citricolor (1996)
    Springer
    World journal of microbiology and biotechnology 12 (1996), S. 97-98 
    Details
    ISSN: 1573-0972
    Keywords: Anti-microbial activity ; Bacillus ; biological control ; coffee disease ; Mycena citricolor
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Process Engineering, Biotechnology, Nutrition Technology
    Notes: Abstract A cell-free supernatant and an ethanolic extract of a 3-day-old culture of Bacillus UCR-236 inhibited the growth of Mycena citricolor, as determined by the ‘Oxford cylinder’ method. A 3-day-old culture of the same bacterium also decreased leaf infection by the pathogen in a moisture-chamber test.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00327810
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