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  • 11
    Electronic Resource
    Electronic Resource
    Molecular characterization of an autolysin-defective mutant of Streptococcus pneumoniae
    Amsterdam : Elsevier
    Biochemical and Biophysical Research Communications 137 (1986), S. 614-619 
    Details
    ISSN: 0006-291X
    Source: Elsevier Journal Backfiles on ScienceDirect 1907 - 2002
    Topics: Biology , Chemistry and Pharmacology , Physics
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1016/0006-291X(86)91122-8
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  • 12
    Electronic Resource
    Electronic Resource
    A new peptidoglycan hydrolase in Streptococcus pneumoniae (1986)
    Oxford, UK : Blackwell Publishing Ltd
    FEMS microbiology letters 35 (1986), S. 0 
    Details
    ISSN: 1574-6968
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Biology
    Notes: Abstract The use of a mutant of Streptococcus pneumoniae deleted in the lytA gene coding for the N-acetyl-muramyl-l-alanine amidase, and therefore devoid of any amidase, has allowed the identification of a new murein hydrolase activity in this bacterium. This enzyme (or enzymes) acted as an autolysin when the cultures were grown at 30°C. Our results strongly suggest that the new lytic activity corresponds to one or more glycosidases.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1111/j.1574-6968.1986.tb01520.x
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  • 13
    Electronic Resource
    Electronic Resource
    Identification of a lytic enzyme of Clostridium acetobutylicum that degrades choline-containing pneumococcal cell walls (1988)
    Oxford, UK : Blackwell Publishing Ltd
    FEMS microbiology letters 52 (1988), S. 0 
    Details
    ISSN: 1574-6968
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Biology
    Notes: Abstract A protein that degrades pneumococcal walls containing choline, but not ethanolamine, in the teichoic acids has been isolated and purified from supernatants obtained from cultures of Clostridium acetobutylicum. The analyses of the degradation products of [3H]choline-labeled cell walls treated with this enzyme indicated that the purified protein, showing an apparent Mr of 115 000, is an N-acetylmuramyl-l-alanine amidase. Our results also suggest that C. acetobutylicum contains choline in its cell wall.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1111/j.1574-6968.1988.tb02584.x
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  • 14
    Electronic Resource
    Electronic Resource
    Darstellung, kristallographische Daten und IR-Spektrum von Mangan(II)-diarsenat (1975)
    Springer
    Monatshefte für Chemie 106 (1975), S. 1559-1566 
    Details
    ISSN: 1434-4475
    Source: Springer Online Journal Archives 1860-2000
    Topics: Chemistry and Pharmacology
    Notes: Abstract Mn2As2O7 has been obtained by solid state reaction between As2O5 and MnCO3 at 600 °C and also by thermal decomposition of NH4MnAsO4·H2O. The crystallographic study has shown that the substance crystallizes in the monoclinic thortveitite structure. Its i.r. spectrum was recorded and discussed in relation with their structural properties. The i.r. spectrum of the parent compound NH4MnAsO4·H2O was also briefly discussed.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00913631
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  • 15
    Electronic Resource
    Electronic Resource
    Isolation, characterization and physiological properties of an autolytic-deficient mutant of Streptococcus pneumoniae (1986)
    Springer
    Molecular genetics and genomics 204 (1986), S. 237-242 
    Details
    ISSN: 1617-4623
    Keywords: Autolysins ; Pneumococcal amidase ; Frame shift mutation ; Recombinant plasmids ; maxicells
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary A spontaneous mutation in the gene lyt encoding the pneumococcal autolysin has been characterized. This mutation, named lyt-32, which behaves as a high-efficiency marker in pneumococcal transformation, is a single base pair GC deletion causing the appearance of two consecutive termination codons in the amino terminal part of the sequence of the autolysin gene. The mutant lyt gene did not code for a polypeptide of relative molecular mass corresponding to the pneumococcal E form amidase in Escherichia coli maxicells. Pneumococcal cells containing the lyt-32 mutation (M32) were fully transformable, multiplied at a normal growth rate forming small chains and showed a tolerant response when treated with beta-lactam antibiotics. Strain M32 represents the first example of a mutant of Streptococcus pneumoniae completely lacking amidase as a consequence of an alteration in the structural gene coding for the pneumococcal autolysin.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00425504
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  • 16
    Electronic Resource
    Electronic Resource
    Neuronal differentiation in the thalamic area triangularis of a lizard (1990)
    New York, NY : Wiley-Blackwell
    Journal of Morphology 205 (1990), S. 123-134 
    Details
    ISSN: 0362-2525
    Keywords: Life and Medical Sciences ; Cell & Developmental Biology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Medicine
    Notes: Development of neurons in the area triangularis of Gallotia galloti was investigated in Golgi-impregnated brain tissue. Four major neuronal types present in adults were found to originate from two migratory neuroblast types, which were followed from embryonic stage S.32. One type has a thick main medial process, whereas the second type has a long main lateral process. As they migrate toward the periphery of the nucleus, morphological characteristics of maturation appear, including growth cones, filopodia, and outgrowth of axons. Neuroblasts with a main lateral process differentiate into two immature neuronal types, bipolars and pyramidals, observed at S.33 and thereafter. The neuroblasts with a main medial process undergo some somatic translocation through a transitory tangential shaft. Then they develop into monopolar immature forms with a long varicose medial, process, appearing from S.36. onward. Immature bipolar neurons do not experience great changes in their dendritic arborization during development to the adult stage, but pyramidals and monopolars undergo a rapid development of the dendritic tree after S.36. By S.38 archetypes of adult neuronal forms are established. Hairlike appendages first appear on neurons at S.36 They decrease suddenly in S.38 and then proliferate in S.39 when spines first appear. Around the time of hatching, the hairlike appendages begin to disappear and spines become established. Reduction of spines occurs after hatching and continues to the adult stage. Possible influences of several external factors on neuronal maturation are discussed.
    Additional Material: 14 Ill.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/jmor.1052050112
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  • 17
    Electronic Resource
    Electronic Resource
    Location of the rostral end of the longitudinal brain axis: Review of an old topic in the light of marking experiments on the closing rostral neuropore (1987)
    New York, NY : Wiley-Blackwell
    Journal of Morphology 194 (1987), S. 163-171 
    Details
    ISSN: 0362-2525
    Keywords: Life and Medical Sciences ; Cell & Developmental Biology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Medicine
    Notes: The rostral end of the forebrain was classically defined on the basis of descriptive data. Different assumptions on the mode of closure of the rostral neuropore caused three different theories of the rostral end of the forebrain to be formulated (His 1893a; von Kupffer, '06; Johnston, '09). Some recent descriptive and experimental data have put these theories into question.A piece of black nylon thread was inserted through the rostral neuropore of chick embryos and was fixed to its ventral lip. These operations were done at all intermediate stages during the process of closure of the rostral neuropore. The embryos were sacrificed at a later stage, by which time the neuropore had disappeared.In the cleared specimens the threads always lay at the same site, namely the upper border of lamina terminalis, irrespective of the stage at which the marker was inserted. These results stand against His's conception ((1893a, b) of a sutura terminalis and support the single mechanism of sutura dorsalis during closure of the rostral neuropore. The marking data therefore imply that the topologic rostral end of the forebrain lies at the upper limit of lamina terminalis, as proposed by von Kupffer, '06).
    Additional Material: 7 Ill.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/jmor.1051940205
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  • 18
    Electronic Resource
    Electronic Resource
    Golgi study of the anterior dorsal ventricular ridge in a lizard. II. Neuronal cytodifferentiation (1990)
    New York, NY : Wiley-Blackwell
    Journal of Morphology 203 (1990), S. 301-310 
    Details
    ISSN: 0362-2525
    Keywords: Life and Medical Sciences ; Cell & Developmental Biology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Medicine
    Notes: The development of the morphologic features of neurons in the anterior dorsal ventricular ridge (ADVR) has been followed in Golgi preparations from the lizard Gallotia galloti between embryonic stage 32 and post-eclosion stages of specimens 3.6-4.5 cm in length. The differentiation sequence of multipolar and bitufted neurons was established. Dendritic growth cones are present after stage 34. Filiform dendritic processes are replaced later on by spines. Clusters of neurons first appear at stage 39 in the periventricular zone, the cells becoming Golgi-impregnated in pairs. After hatching, the number of impregnated cells per cluster increases.
    Additional Material: 9 Ill.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/jmor.1052030305
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  • 19
    Electronic Resource
    Electronic Resource
    Golgi study of the anterior dorsal ventricular ridge in a lizard. I. neuronal typology in the adult (1990)
    New York, NY : Wiley-Blackwell
    Journal of Morphology 203 (1990), S. 293-300 
    Details
    ISSN: 0362-2525
    Keywords: Life and Medical Sciences ; Cell & Developmental Biology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Medicine
    Notes: Using Golgi techniques we have studied neuronal cell types in the anterior dorsal ventricular ridge (ADVR) of the adult lizard Gallotia galloti. Multipolar, bitufted, and juxtaependymal neuronal forms were found. The multipolar and bitufted neurons are present in both the periventricular and central ADVR zones. Multipolar neurons can be subdivided into multipolar neurons with polygonal somata and four to six main dendritic trunks and multipolar neurons with pyramidal somata and three or more dendritic trunks. The former are the cells most frequently impregnated in the ADVR. In the population of bitufted neurons, we distinguish subtypes I, II, and III according to the number of dendritic trunks that emerge from the somata. Juxtaependymal neurons are restricted to a cell-poor zone, adjacent to ependymal cells. Their dendrites either are orientated parallel to the ventricular surface or extend into the periventricular zone. The dendrites of ADVR neurons have pedunculated spines with knob-like tips. However, such spines do not appear on the somata or on the primary dendritic trunks. The number of spines is scarce or moderate. The periventricular neuronal clusters contain two to five cells. The morphology of these neurons is mainly multipolar, but we also found some bitufted neurons.
    Additional Material: 4 Ill.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/jmor.1052030304
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  • 20
    Electronic Resource
    Electronic Resource
    Neuronal typology of the thalamic area triangularis of Gallotia galloti (reptilia, sauria) (1990)
    New York, NY : Wiley-Blackwell
    Journal of Morphology 205 (1990), S. 113-121 
    Details
    ISSN: 0362-2525
    Keywords: Life and Medical Sciences ; Cell & Developmental Biology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Medicine
    Notes: In a Golgi study of the area triangularis (AT), a rostral nucleus of the ventral thalamus of Gallotia galloti, we have identified four major neuronal types on the basis of their morphological characteristics: medium-sized fusiforms with two processes, medium-sized fusiforms with three or four processes, small bipolars, and small and medium-sized multipolars. These neurons are characterized by a simple morphology and radial arrangement. Cell size varies from small to medium, and all axons project laterally. These characteristics distinguish AT neurons from those of neighboring nuclei. In addition, we found some evidence of differential topographic distribution of each neuronal type within the nucleus. Medium-sized fusiform neurons with two processes are located in the most ventral part, where they constitute the ventral nuclear limit. Small multipolar neurons prevail in the dorsal and ventromedial parts, and in the rest of the nucleus medium-sized neurons, including both fusiform with three or four processes and multipolar types, are normally found. Finally, we discuss a putative homology of the reptilian AT with a part of the mammalian zona incerta.
    Additional Material: 10 Ill.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/jmor.1052050111
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