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  • 11
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    Influence of lead nitrate upon organogenesis of Hypophthalmichthys molitrix (2021)
    In:  http://aquaticcommons.org/id/eprint/23546 | 18721 | 2018-07-03 14:57:55 | 23546 | Iranian Fisheries Science Research Institute
    Details
    Publication Date: 2021-07-14
    Description: Eggs of Hypophthalmichthys molitrix have been subjected to lead nitrate in dosages of 0.01 ppm up to 20 ppm and it was observed that sample of more than 12 hours before were resistant to lead nitrate. Also it was observed that is sample of more orc than 32 hours lead increased mortality rate and abnormalities. These samples lost body length avid accumulated lead in their tissues. Lead affected thickness of retina layers in the eyes of swim fry's and in dosages of 1 to 20 ppm decreased thickness of these layers from 6% to 12%, Nearly 50 percent of new larvae in dosage of 1 ppm and 100 percent of them in dosage of 20 ppm showed symptoms of lordosis is abnormality. No meaningful histopathological effect was seen by affecting lead nitrate on eye lenses optic capsule, ventricles, notochord and yolk sac of these larvae, Average lead nitrate concentration in Anzali Wetland is 0.124+0.0507 ppm in surface waters and 0.1956t+0.385 ppm in deeper waters. This level of lead pollution in Azali Wetland caused 8 percent of silver carp fertilized eggs to death and it is demonstrated that at this rate of pollution nearly I8 percent of these eggs will be valueless from the fishery viewpoint.
    Keywords: Biology ; Fisheries ; Nitrate ; Eggs ; Tissues ; Lead ; Surface ; Pollution ; Organogenesis ; Histopathological ; Hypophthalmichthys molitrix ; Anzali Wetland ; Iran
    Repository Name: AquaDocs
    Type: article , TRUE
    Format: application/pdf
    Format: application/pdf
    Format: 31-46
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  • 12
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    Chitosan extracted from the Persian Gulf chiton shells: Induction of apoptosis in liver cancer cell line (2021)
    In:  http://aquaticcommons.org/id/eprint/22958 | 18721 | 2018-06-07 21:05:01 | 22958 | Iranian Fisheries Science Research Institute
    Details
    Publication Date: 2021-07-11
    Description: Here for the first time, we investigated the cytotoxic effects of the chitosan extracted from the Persian Gulf Chiton shell (Acanthopleura vaillantii) on liver cancer cell line (HepG_2). Chitosan extraction was implemented following this method: chitin was produced by demineralization and deproteinization procedure, and the extracted chitin was converted into soluble chitosan using deacetylation method. The cytotoxic effects of extracted chitosan were evaluated using four different tests, including 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay, Annexin V-FITC, propidium iodide (PI) staining, 4',6-diamidino-2-phenylindole (DAPI) staining, and Caspase activity analysis. The IC_50 inhibitory concentrations of chitosan were obtained at 250 µg/mL after 24 h. Chitosan clearly inhibited the growth of hepatocarcinoma cells in vitro in a dose-dependent manner. For detecting the induced cell apoptosis, HepG_2 cells were treated with 125, 250 and 500 µg/ml of chitosan for 24 h. According to the result of Annex in V/PI kit, in 125, 250, and 500 µg/ml of chitosan, 28.2, 49.1, and 83.3% of HepG_2 cells undergone late apoptosis, respectively. The morphology of treated cells by DAPI staining showed non uniform plasma membrane and DNA fragmentation compared to untreated cells with perfect nucleus. The analysis of cell cycle using flow cytometry demonstrated that the rate of sub-G1 peak was increased to 52.7%. Both caspase-3 and -9 activities increased by the extracted chitosan, but it was only significant for caspase-3. The results of the present study suggested that the extracted chitosan has efficient cytotoxicity on HepG_2 cells. Therefore, the extracted chitosan from the shell of the Chiton may be considered as a futuristic natural product regarding the treatment of liver cancer.
    Keywords: Biology ; Chemistry ; Health ; Chiton ; Chitosan ; Persian Gulf ; Natural product ; Apoptosis ; Biology ; Physiology ; Iran
    Repository Name: AquaDocs
    Type: article , TRUE
    Format: application/pdf
    Format: application/pdf
    Format: 1362-1378
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  • 13
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    Survey on Acipenser persicus populations by use of the electrophoretic technique and morphological characteristics (2021)
    In:  http://aquaticcommons.org/id/eprint/23892 | 18721 | 2018-08-01 08:02:09 | 23892 | Iranian Fisheries Science Research Institute
    Details
    Publication Date: 2021-07-15
    Description: For identifying the populations of the Acipenser persicus (Persian sturgeon) a survey were done by use of the electrophoretic technique and also morphological characteristics. The matrix was Acetate cellolus. 5ml blood from each gill arch were obtained and separated serum from blood. Separation of proteins were done, using a voltage of 180 volt during 15 minutes. After separating the solution proteins, the bands were stained by ponceau-s, Decolouring of plates was done by Acetic acid 5% during three steps. Scanning of the plates was done by densitometer with the wave length of 525 nm. Several genetic variations observed in the electrophorograms of samples. It seems that population of some localities can be characterized as a genetically distinguishable unit. Morphological differences observed between the fish which located in Sephid Rud region and Gorgan region, like the eye diameter and head height. The mean of eye diameter in Sephid Rud population was 20.16 mm and in Gorgan population 22.47 mm and the mean of head height was 8.15 cm and 7.25 cm respectively.
    Keywords: Fisheries ; Survey ; Acipenser persicus ; Population ; Electrophoretic Technique ; Morphological Characteristics ; Persian sturgeon ; Matrix ; Acetate cellolus ; Gill ; Blood serum ; Proteins ; Solution ; Ponceau-s ; Decolouring ; Acetic acid ; Scanning ; densitometer ; Length ; Genetic variation ; Electrophorograms ; Fish ; Eye diameter ; Head height ; Characterized ; Genetically ; Distinguishable unit ; Gorgan ; Sephid Rud ; Iran
    Repository Name: AquaDocs
    Type: article , TRUE
    Format: application/pdf
    Format: application/pdf
    Format: 41-50
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  • 14
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    Determination of the spawning time and changes in the reproduction cycle of ribbon fish, Trichiurus lepturus, according to the hepatosomatic index and gonadosomatic index (2021)
    In:  http://aquaticcommons.org/id/eprint/23915 | 18721 | 2018-08-01 16:28:45 | 23915 | Iranian Fisheries Science Research Institute
    Details
    Publication Date: 2021-07-15
    Description: The ribbon fishes, belonging to the family Trichiuridae, are one of the most important protein resources in the Indian Ocean. A considerable density of the dominant species of this family, Trichiurus lepturus has been observed in the Oman Sea. During the conducted cruises in the northern part of the Oman Sea, gonadosomatic index (GSI) and hepatosomatic index (HSI) were estimated according to the size of the female groups of Trichiurus lepturus. GSI increases significantly from September to next April (P 〈0.05). polymodal egg size frequency revealed prolong spawning time by the females. The HSI fluctuations are good indications for this type of reproductive behavior.
    Keywords: Biology ; Density ; Species ; Spawning ; Gonadosomatic ; Hepatosomatic ; Size ; Eggs ; Fish ; Protein ; Determination ; Reproduction ; Trichiuridae ; Trichiurus ; Oman Sea ; Indian Ocean ; Iran
    Repository Name: AquaDocs
    Type: article , TRUE
    Format: application/pdf
    Format: application/pdf
    Format: 63-74
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  • 15
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    Sex hormones changes in the reproductive cycle of female Trichiurus lepturus (2021)
    In:  http://aquaticcommons.org/id/eprint/23943 | 18721 | 2018-08-01 17:17:40 | 23943 | Iranian Fisheries Science Research Institute
    Details
    Publication Date: 2021-07-15
    Description: Ribbon fish, family Trichiuridae, is one the most important protein resources of the Indian Ocean. The considerable density of these aquatic animals, especially the dominant species Trichiurus lepturus, has drawn many researchers' attention to its biological characteristics and its reproductive characteristics in particular. The sampling was carried out in the Oman Sea from March 95 to November 96 (n=778). Studies on Estradiol - 17 - β, Progesterone and Cortisol hormones in female Trichiurus lepturus indicated that the production of these hormones increases considerably from maturity stage II. The serum levels of Estradiol - 17 - β hormone peaked during September, October and November and it remained high in the other months (1500 pg/ml). High levels of Progesterone occurred from December to March (during the spawning season). The changes in the Cortisol levels from August to December and again from February to March (0.5 - 3.5 mic/100 ml) were significant. The Estradiol - 17 - β fluctuation was observed simultaneously with the vitellogenesis process and absorption of Vitelline by the oocytes during their maturation. The changes in the oocytes membrane as well as the perforated channels impact on serum levels of Estradiol - 17 - β and Cortisol hormones have been discussed in this paper. The relative high levels of Estradiol - 17 - β during the whole year is probably related to the long spawning season of this species during autumn, winter and spring.
    Keywords: Aquaculture ; Biology ; Sex ; Hormone ; Female ; Trichiurus lepturus ; Ribbon fish ; Trichiuridae ; Protein ; Density ; Aquatic ; Species ; Estradiol ; Progesterone ; Serum level ; Spawning ; Fluctuation ; Vitellogenesis ; Absorption ; Oocytes ; Biological ; Reproductive ; Estradiol ; Oman Sea ; Iran
    Repository Name: AquaDocs
    Type: article , TRUE
    Format: application/pdf
    Format: application/pdf
    Format: 49-68
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  • 16
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    Effects of NaCl stress on blood glucose and cortisol in common crap (Cyprinus carpio) (2021)
    In:  http://aquaticcommons.org/id/eprint/24781 | 18721 | 2018-08-06 08:02:39 | 24781 | Iranian Fisheries Science Research Institute
    Details
    Publication Date: 2021-07-15
    Description: In this study, the fluctuation of two parameters of blood glucose and cortisol hormone were investigated in different salinities in Cyprinus carpio. Seven tanks with 100 lit. volume were used and different salinities of 0, 3, 6, 12, 15 and 18 ppm were choosen as treatments. Then, 16 specimen of common carps (each 50-90 g weight) were added to the tanks. In periBds of 12 up to 96 hours, the blood sample was taken from each fish for further measurment of parameters. The results showed that all fishes died at 18 ppm salinity within less than 12 hours. The blood glucose indicated high sensitivity to the higher salinity. In this research, blood glucose had a reange of 13.5 to 321.0 mg/dc.lit and also, cortisol showed a fluctuation from 10 up to 70 µg/dc. lit and its concentration had a direct relationship with increasing the salinity.
    Keywords: Biology ; Blood Glucose ; Cortisol ; Salinity ; Common carp ; Cyprinus carpio ; Iran
    Repository Name: AquaDocs
    Type: article , TRUE
    Format: application/pdf
    Format: application/pdf
    Format: 35-42
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  • 17
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    Effects of NaCl stress on blood Glucose and Cortisol in Common crap (Cyprinus carpio) (2018)
    Details
    Publication Date: 2021-05-19
    Description: In this study, the fluctuation of two parameters of blood glucose and cortisol hormone were investigated in different salinities in Cyprinus carpio. Seven tanks with 100 lit. volume were used and different salinities of 0, 3, 6, 12, 15 and 18 ppm were choosen as treatments. Then, 16 specimen of common carps (each 50-90 g weight) were added to the tanks. In periBds of 12 up to 96 hours, the blood sample was taken from each fish for further measurment of parameters. The results showed that all fishes died at 18 ppm salinity within less than 12 hours. The blood glucose indicated high sensitivity to the higher salinity. In this research, blood glucose had a reange of 13.5 to 321.0 mg/dc.lit and also, cortisol showed a fluctuation from 10 up to 70 µg/dc. lit and its concentration had a direct relationship with increasing the salinity.
    Description: Published
    Keywords: Blood Glucose ; Cortisol ; Salinity ; Common carp ; Cyprinus carpio ; Hormone ; Specimen
    Repository Name: AquaDocs
    Type: Journal Contribution , Refereed
    Format: pp.35-42
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