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  • 11
    ISSN: 1573-5117
    Keywords: mate recognition ; pheromones ; antibody ; reproductive isolation ; rotifers
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract The work of Kazutsugu Hirayama over the past 25 yearspromoted the wide use of the marine rotifer Brachionusplicatilis as an experimental model in zooplanktonecology. His reports about the nature of geneticvariation in the B. plicatilis complex stimulated usto investigate how mate recognition maintains speciesboundaries. For the past several years, we haveexamined chemical communication between female andmale B. plicatilis. Here we report on the comparativebinding of polyclonal antibody against the materecognition pheromone (MRP) to three B. plicatilisstrains and three B. rotundiformis strains.Quantification of anti-MRP binding permitsinvestigation of how the female mating signal differsamong closely related Brachionus species and strains.Antibody binding reflects differentiation independentof the male receptor which has been describedelsewhere. Anti-MRP bound to females of all sixstrains and was localized in the corona. Antibodybinding greatly reduced mating in all three B. plicatilis strains. However, antibody bindingsignificantly reduced mating in only one of the B. rotundiformis strains. The MRP of both species has asimilar molecular weight, but the differential bindingsuggests that the mate recognition pheromone onfemales has differentiated in B. plicatilis and B. rotundiformis.
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  • 12
    Electronic Resource
    Electronic Resource
    Springer
    Hydrobiologia 387-388 (1998), S. 109-115 
    ISSN: 1573-5117
    Keywords: behavior ; mating ; evolution ; taxonomy
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract The recent isolation of a mate recognition pheromone in the marine rotifer Brachionus plicatilis Müller has shed new light on the mate recognition system of rotifers. One result is improved understanding of the importance of mating behavior as a highly efficient process used by rotifers to choose conspecifics. There are many differences in the main characteristics of mating behavior in members of five different families of rotifers. The present work describes the use of these characteristics to assess species boundaries, especially where boundaries between two or more species are unclear. The method proposed here can assess quantitatively the response of males of one species to females of a questionable taxon by measuring the percentage of matings initiated and the number of completed copulations. The data generated can then be used together with molecular, morphological, and other data to determine the species boundaries. This approach can help distinguish between morphological differences resulting from evolutionary divergence of species and morphological differences induced by environmental or ecological factors.
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  • 13
    Electronic Resource
    Electronic Resource
    Springer
    Hydrobiologia 387-388 (1998), S. 341-348 
    ISSN: 1573-5117
    Keywords: Lecanidae ; life-table ; mortality ; reproduction rate ; survivorship
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract We performed life-table experiments with two species of the littoral rotifers Lecane luna (O.F. Müller, 1776) and Lecane quadridentata (Ehrenberg, 1832). Three different temperatures (20, 25 and 30°C) and food concentrations of Nannochloris oculata (1×107, 5×106, and 1×106 cells ml-1) were investigated. We found important differences between both species in all the treatments regarding offspring sizes, hatching percentages, life span and reproductive rates. Our data on hatching percentages of asexual eggs suggested that the optimal temperature for both species is in the 20–25°C range. On the other hand, reproductive data placed the optimal temperature near 25°C. This information can be used to develop aquatic toxicology tests with littoral species.
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  • 14
    Electronic Resource
    Electronic Resource
    Springer
    Hydrobiologia 356 (1997), S. 165-173 
    ISSN: 1573-5117
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract We characterized the mating behavior of eight freshwater rotifer species from five different families of Monogononts (Asplanchnidae, Brachionidae, Euchlanidae, Lecanidae, and Trichocercidae). Were corded the percentages of male-female encounters resulting in mating attempts and the number of completed copulations. The characterization of mating behavior also included data on: (1) duration of copulation, (2) female and male swimming speed, (3) sites of initial mating attempts, (4) sites of copulation, and (5) sizes of males and females. In the five families investigated, cross-mating tests may be used to assess the ability of males to discriminate conspecifics, establishing limits to gene flow. We also present data on two families, Brachionidae and Lecanidae where we used cross-mating tests to compare discrimination between conspecific females and those of other species. Brachionus calyciflorus males attempted mating with females of the closely related genus Plationus, but did not attempt mating with Keratella Americana females. Males of other three Brachionidae species only attempted mating with nonspecific females. Lecane quadridentata males attempted to mate with females of two different Lecane species besides their own. Percentages of mating attempts and completed copulations were quantified in all crosses. Lower percentages of copulation after a mating attempt were found among brachionids than among non-brachionids. These data demonstrate that male discrimination of females is an effective pre-mating reproductive barrier in several species of Monogonont rotifers. These results, further suggest that investigations of mating behavior are useful for determining species boundaries in rotifers and perhaps other zooplankters.
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  • 15
    Electronic Resource
    Electronic Resource
    Springer
    Hydrobiologia 287-388 (1998), S. 109-115 
    ISSN: 1573-5117
    Keywords: behavior ; mating ; evolution ; taxonomy
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract The recent isolation of a mate recognition pheromone in the marine rotifer Brachionus plicatilis Müller has shed new light on the mate recognition system of rotifers. One result is improved understanding of the importance of mating behavior as a highly efficient process used by rotifers to choose conspecifics. There are many differences in the main characteristics of mating behavior in members of five different families of rotifers. The present work describes the use of these characteristics to assess species boundaries, especially where boundaries between two or more species are unclear. The method proposed here can assess quantitatively the response of males of one species to females of a questionable taxon by measuring the percentage of matings initiated and the number of completed copulations. The data generated can then be used together with molecular, morphological, and other data to determine the species boundaries. This approach can help distinguish between morphological differences resulting from evolutionary divergence of species and morphological differences induced by environmental or ecological factors.
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  • 16
    Electronic Resource
    Electronic Resource
    Springer
    Hydrobiologia 255-256 (1993), S. 467-474 
    ISSN: 1573-5117
    Keywords: Rotifera ; zoogeography ; Mexico
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract This faunistic survey of Mexican Rotifera, covering 32 localities, mostly in Central Mexico, has yielded 96 taxa of which 41 are new to Mexican fauna. The zoogeographic status of the rotifer fauna of Mexico was reevaluated on the basis of the present data.
    Type of Medium: Electronic Resource
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  • 17
    Electronic Resource
    Electronic Resource
    Springer
    Hydrobiologia 287-388 (1998), S. 341-348 
    ISSN: 1573-5117
    Keywords: Lecanidae ; life-table ; mortality ; reproduction rate ; survivorship
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract We performed life-table experiments with two species of the littoral rotifers Lecane luna (O.F. Müller, 1776) and Lecane quadridentata (Ehrenberg, 1832). Three different temperatures (20, 25 and 30°C) and food concentrations of Nannochloris oculata (1×107, 5×106, and 1×106 cells ml-1) were investigated. We found important differences between both species in all the treatments regarding offspring sizes, hatching percentages, life span and reproductive rates. Our data on hatching percentages of asexual eggs suggested that the optimal temperature for both species is in the 20–25°C range. On the other hand, reproductive data placed the optimal temperature near 25°C. This information can be used to develop aquatic toxicology tests with littoral species.
    Type of Medium: Electronic Resource
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  • 18
    Electronic Resource
    Electronic Resource
    New York, NY [u.a.] : Wiley-Blackwell
    Chirality 8 (1996), S. 311-315 
    ISSN: 0899-0042
    Keywords: virtual triligand chiral center ; chirality of saturated heterocycles with six-membered ring ; rules for the specification of the configuration ; desymmetrization of adamantane ; Chemistry ; Organic Chemistry
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Chemistry and Pharmacology
    Notes: The chirality of the title heterocycles is discussed considering their genesis by desymmetrization of the corresponding adamantanes. Some rules for the specification of the absolute configurations of the enantiomers (R or S) for this type of compounds are proposed. © 1996 Wiley-Liss, Inc.
    Additional Material: 10 Ill.
    Type of Medium: Electronic Resource
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  • 19
    ISSN: 0947-3440
    Keywords: Conformational analysis ; NMR spectra ; Solid-state structure ; 1,3-Dioxane derivatives of o-phthaldialdehyde ; Chromatographic separation of cis,trans isomers ; Chemistry ; Organic Chemistry
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Chemistry and Pharmacology
    Notes: The stereochemistry of some diacetals of o-phthaldialdehyde with 2-substituted-1,3-propanediols was studied using NMR spectroscopy and the solid-state molecular structure of one compound established by single-crystal X-ray diffractometry. The cis and trans isomers of some diacids and diesters, produced as derivatives bearing the desired substituents in the 5 and 5′ positions of the heterocycles, have been separated by preparative TLC and identified by their NMR spectra.
    Additional Material: 4 Ill.
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  • 20
    Electronic Resource
    Electronic Resource
    Chichester : Wiley-Blackwell
    Biological Mass Spectrometry 26 (1991), S. 1095-1096 
    ISSN: 0030-493X
    Keywords: Chemistry ; Analytical Chemistry and Spectroscopy
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Chemistry and Pharmacology
    Notes: The electron impact mass spectra of thirteen new substituted 2-(o-R1-phenyl)-indole-3-carboxaldehydes which have potentially useful pharmacological properties, ate presented.
    Additional Material: 1 Tab.
    Type of Medium: Electronic Resource
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