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  • 11
    Electronic Resource
    Electronic Resource
    Purification and characterization of variant alcohol dehydrogenase isozymes from durum wheat (1987)
    Springer
    Biochemical genetics 25 (1987), S. 581-590 
    Details
    ISSN: 1573-4927
    Keywords: alcohol dehydrogenase ; durum wheat ; isozymes ; enzyme purification
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Chemistry and Pharmacology
    Notes: Abstract Three alcohol dehydrogenase (ADH) isozymes from embryos of the durum wheat cultivar Bijaga Yellow having the variantAdh-Alb allele were purified using (NH4)2SO4 precipitation, gel filtration, and ion-exchange chromatography. ADH is a dimeric enzyme. The variant isozyme ADH-1-1, which is a homodimer composed of αb monomers, was compared with ADH-1-5 (homodimer composed of βa monomers), the product ofAdh-B1, and the ADH-1-3 isozyme (αbβa heterodimer) on a number of parameters includingK m, substrate specificities, and molecular weights. No appreciable differences among the three isozymes were found, except for the faster electrophoretic mobility of αbαb dimers (ADH-1-1). The results indicate that the variant isozyme is the result of a mutation altering only the charge of the isozyme.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00554359
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  • 12
    Electronic Resource
    Electronic Resource
    Isoenzyme changes accompanying germination of wheat seeds (1969)
    Springer
    Biochemical genetics 3 (1969), S. 207-214 
    Details
    ISSN: 1573-4927
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Chemistry and Pharmacology
    Notes: Abstract Electrophoretic patterns of soluble proteins and enzymes during development from dry seed to first leaf were studied in wheat by means of disc electrophoresis. The patterns considered were those of alcohol dehydrogenase (ADH), peroxidase, and nonspecific esterases. Multiple forms (isoenzymes) were found for all these enzymes in dry seeds. The ADH pattern did not change during development. Esterases and peroxidase patterns changed both qualitatively and quantitatively. Increase in the number of peroxidase bands was marked.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00521137
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  • 13
    Electronic Resource
    Electronic Resource
    Adaptation and growth response of Arabidopsis thaliana to deuterium (1968)
    Springer
    Planta 80 (1968), S. 176-184 
    Details
    ISSN: 1432-2048
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary Arabidopsis seeds were sown aseptically on mineral media containing between 0 and 90% of heavy water (D2O). Initially, a D2O level of over 50% was lethal for the plants. However, after culture for six successive generations on 50% D2O, plants were capable of growing marginally on media containing up to 70% D2O, but not higher. With increasing concentration, deuterium progressively delays germination, slows growth, reduces survival, results in bleaching of the leaves and delays flowering. Pollen fertility is not affected measurably but seed set is reduced with increasing levels of deuteration so that at 70% D2O few seeds were obtained. The viability of the seeds harvested from plants grown on deuterated media is low. No chlorophyll or morphological mutants were observed among a large number of plant progenies. Seeds from plants cultured on D2O media for several generations grow normally on proteated media in the very first generation.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00385593
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  • 14
    Electronic Resource
    Electronic Resource
    Alteration of reproductive effort by a monogenic, recessive mutation in jute (Corchorus capsularis L.) (1991)
    Springer
    Theoretical and applied genetics 82 (1991), S. 254-255 
    Details
    ISSN: 1432-2242
    Keywords: Jute ; Reproductive effort ; Monogenic
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary Reproductive effort (RE) of a day-length neutral mutant TCJ-5, its parent, and two other cultivars of jute (Corchorus capsularis) was estimated as reproductive biomass/aerial biomass. Plant height at flowering and aerial biomass were significantly higher in the mutant, while the reproductive biomass at 55 days after flowering was statistically equal. Therefore, the estimated RE was significantly lower in the mutant compared to the parent and other cultivars. The lower RE of the mutant was due to delayed initiation of flowering and additional vegetative growth in this period. The results show alteration of RE by a recessive mutation.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00226222
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  • 15
    Electronic Resource
    Electronic Resource
    Inheritance of powdery mildew (Erysiphe polygoni DC) resistance in mungbean (Vigna radiata L. Wilczek) (1994)
    Springer
    Theoretical and applied genetics 88 (1994), S. 945-948 
    Details
    ISSN: 1432-2242
    Keywords: Mungbean ; Erysiphe polygoni ; Inheritance Powdery mildew ; Resistance
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Detached mungbean (Vigna radiata L.Wilczek) leaves were inoculated with a conidial suspension of a local isolate (TI-1) of the powdery mildew pathogen (Erysiphe polygoni DC) under controlled environment conditions. Based on the latent period and severity of the infection, a rating scale of 0–5 was used to classify the host pathogen interactions. Reactions 0, 1 and 2 were considered resistant and referred to as R0, R1 and R2 while 3, 4 and 5 were classified as susceptible (S). RUM lines (resistant to powdery mildew) and their derivatives are crossed with several susceptible (reaction types 3–5) genotypes and the inheritance of the resistance was studied in the F1, F2 and F3 generations. The results showed that powdery mildew resistance in mungbean is governed by two dominant genes designated as Pm-1 and Pm-2. When both Pm-1 and Pm-2 were present, an R0 reaction was observed after inoculation with TI-1. The resistant reaction was R1 when only Pm-1 was present and R2 in the presence of Pm-2. In the absence of both Pm-1 and Pm-2, susceptible reactions 3, 4 and 5 were observed.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00220800
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  • 16
    Electronic Resource
    Electronic Resource
    Effect of rye chromosome 2 substitution on kernel protein content of wheat (1972)
    Springer
    Theoretical and applied genetics 42 (1972), S. 89-92 
    Details
    ISSN: 1432-2242
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary Significant increases in the kernel protein content of lines of the bread wheat variety Chinese Spring, in which a pair of rye chromosome 2 substitutes for group 2 chromosomes of wheat, were observed in plants grown at three different locations. Fractionation of proteins on the basis of their solubility did not show any significant variation in the relative proportions of alcohol, salt, acid and alkali soluble proteins. Similarly, electrophoretic studies of saline, alcohol and acetic acid-urea soluble proteins did not reveal any addition or deletion of protein bands in comparison with the Chinese Spring control. Apparently, the substituted rye chromosomes do not contribute any proteins different from those already present in wheat. The electrophoretic data give further evidence of the homoeology of rye chromosome 2 with the group 2 chromosomes of wheat.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00277949
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  • 17
    Electronic Resource
    Electronic Resource
    Intervarietal differences in some metabolic functions associated with protein accumulation in rice grains (1976)
    Springer
    Theoretical and applied genetics 48 (1976), S. 145-151 
    Details
    ISSN: 1432-2242
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary Total nitrogen, amino nitrogen, glutamic acid dehydrogenase (GDH) activity and incorporation of 3H-uridine and 14C-amino acids into RNA and proteins, respectively, were compared in the developing grains of three high-protein stocks (IR-480-5-9, GMPR-51 and Erythroceros) and a high-yielding, medium-protein cultivar IR-8. The above parameters were also independently studied in the developing grains of IR-8 grown at 0, 60 and 120 kgN/ha. In addition, mobilization of nitrogen from flag leaf during kernel development was compared in a separate experiment. Higher protein concentration, both in high-protein stocks and in IR-8 grown at 120 kgN/ha, was associated with increased levels of: soluble amino nitrogen, GDH activity, 3H-uridine and 14C-amino acid incorporation. Significant variation was found among the high protein stocks in mobilization of nitrogen from flag leaf.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00281657
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  • 18
    Electronic Resource
    Electronic Resource
    Bioenergetic considerations in the improvement of oil content and quality in oil-seed crops (1979)
    Springer
    Theoretical and applied genetics 54 (1979), S. 41-47 
    Details
    ISSN: 1432-2242
    Keywords: Production values ; Fatty acids ; Oil ; seed Crops
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary Production values (PVs), defined as the weight of the end product/weight of the substrate required for carbon skeletons and energy production, were calculated for plant fatty acids. The PVs varied from 0.361 to 0.300 with linolenic acid having the lowest value. In general, the PVs of unsaturated fatty acids were lower than those of saturated fatty acids of similar chain lengths. Using this basic information, PVs of (A) oils from different oilseed crops, based on their standard fatty acid composition and (B) seed biomass with specified oil content and fatty acid composition were calculated. 1/PV gives the glucose required for the biosynthesis of 1 g end product and thus an estimate of the photosynthate requirement for the desired breeding goal can be estimated. Such calculations show that increasing oil percentage in seeds has a maximum energy cost when the increase in oil is associated with a decrease in the amount of carbohydrates where there is no change in protein concentration. Reduction of erucic acid content in the rapeseed oil did not alter its PV. It is inferred that there are no serious bioenergetic constraints in altering the fatty acid composition.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00265707
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  • 19
    Electronic Resource
    Electronic Resource
    The basis for grain yield differences in mungbean cultivars and identifications of yield limiting factors (1980)
    Springer
    Theoretical and applied genetics 58 (1980), S. 171-175 
    Details
    ISSN: 1432-2242
    Keywords: Vigna radiata Mungbean ; Grain yield ; Harvest index ; Growth analysis
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary Eight mungbean cultivars, selected from a cultivar collection on the basis of their grain yield, were grown in a replicated experiment. Morphological and physiological components contributing to grain yield were analysed. The principal yield limiting factor and the desirable yield component of each cultivar have been identified. The rate of dry matter accumulation was low in all cultivars. It is suggested that for a short duration crop like this, selection for rapid rate of dry matter increase would be advantageous. However, it should also be associated with a high partitioning efficiency (Harvest index). The top yielding cultivar had high biological yield and productive racemes.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00279709
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  • 20
    Electronic Resource
    Electronic Resource
    Two NAD-dependent alcohol dehydrogenases (E.C. 1.1.1.1) in callus cultures of wheat, rye and triticale (1982)
    Springer
    Theoretical and applied genetics 62 (1982), S. 45-48 
    Details
    ISSN: 1432-2242
    Keywords: Wheat ; Rye ; Triticale ; Callus cultures ; Alcohol dehydrogenase ; Isoenzymes
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary Two NAD-dependent alcohol dehydrogenases ADH-1 and ADH-2, under independent genetic control of genes designated as Adh-1 and Adh-2 located on chromosomes 4A, 4B and 4D, have been reported in aestivum wheat (Hart 1980). Only ADH-1 is expressed in developing seeds, dry seeds, pollen and germinating seedlings. ADH-2 can be induced in seedling roots or shoots under conditions of partial anaerobiosis or by certain chemicals. Expression of ADH-1 and ADH-2 isoenzymes was investigated in undifferentiated calli from aestivum and durum wheats, rye, triticale and also in in vitro regenerated roots and leaves from aestivum cultures. Wheat callus cultures originating from seed, mature and immature embryos, mesocotyl and root, as well as cultures grown on media containing different supplements did not show any variation in the overall expression of ADH-1 or ADH-2, although differences in the band intensities were observed. The callus isoenzyme pattern was similar to that observed in roots under anaerobic conditions. Both ADH-1 and ADH-2 were expressed in in vitro regenerated roots but were absent in regenerated leaves. Expression of ADH-1 and ADH-2 in wheat calli seems to be related to the type of differentiation.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00276281
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