ISSN:
1013-9826
Source:
Scientific.Net: Materials Science & Technology / Trans Tech Publications Archiv 1984-2008
Topics:
Mechanical Engineering, Materials Science, Production Engineering, Mining and Metallurgy, Traffic Engineering, Precision Mechanics
Notes:
In this study, the surface modification and biocompatibility of the biologic chitosanscaffold were investigated. The chitosan scaffold with excellent reticular structure was attained afterbeing purified, emulsionized, cross-linked, molded and freeze-dried step by step by using the nativematerials, coming from such as lobster shell, crab shell etc.. After that, its surface modification wasoperated with film coating by using gelatin. Then the bone marrow mesenchymal stem cells (BMSCs)derived from New Zealand rabbits were used as the seed cells and were inoculated onto the modifiedbiologic chitosan scaffolds at 3×105 cells/ml to investigate the biocompatibility and bone conductiveefficiency of this kind of scaffold in static culture for one week. As a control, the cell suspensions withsame densities were inoculated onto the chitosan scaffold without being treated. During the wholeculture process, the cellular adherence and expansion were observed under inverted microscope.After culture, the biological properties of the fabricated cell-scaffold tissues were detected byscanning electron microscope (SEM) and HO/PI fluorescent double staining. The results showed thatthe biologic chitosan scaffold treated with gelatin or rat-tail collagen promoted a higher adhesion andproliferation of BMSCs in comparison to the untreated samples. Besides, the BMSCs within thetreated scaffold were more regular and well-distributed than those in untreated one. It is concludedthat this kind of surface modification can be used to change the physicochemical properties ofchitosan scaffold. The improved biologic chitosan scaffold is suitable to be an ideal biomedicalscaffold for tissue engineering
Type of Medium:
Electronic Resource
URL:
http://www.tib-hannover.de/fulltexts/2011/0528/01/57/transtech_doi~10.4028%252Fwww.scientific.net%252FKEM.373-374.654.pdf
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