ISSN:
1432-1424
Quelle:
Springer Online Journal Archives 1860-2000
Thema:
Biologie
,
Chemie und Pharmazie
Notizen:
Summary Intracellular chloride activities, (Cl) c , and the electrical potential difference across the mucosal membrane, ψmc, were determined in the isolated small intestine of the winter flounder, using Cl-selective and conventional (KCl-filled) microelectrodes. In the presence of a Na-containing buffer ψmc averages −69mV and (Cl) c averages 24mM, a value that is 3.4 times that predicted for an equilibrium distribution across the mucosal membrane. On the other hand, when the tissue is then perfused with Na-free buffer, (Cl) c slowly falls to a value that does not differ significantly from that predicted for an equilibrium distribution, and ψmc depolarizes significantly. Finally, when the tissue is again bathed in the Na-containing buffer, (Cl) c rapidly returns to a value well above equilibrium. These results, together with those of Frizzellet al. (J. Membrane Biol. 46:27, 1979), provide direct evidence that: (1) Cl is accumulated against its electrochemical potential difference (32mV) by this tissue, and (2) this accumulation is coupled to and energized by the entry of Na down its steep electrochemical potential difference.
Materialart:
Digitale Medien
URL:
http://dx.doi.org/10.1007/BF01868896
Permalink